TITLE

Effects of bone fracture and surgery on plasma myosin heavy chain fragments of skeletal muscle

AUTHOR(S)
Onuoha, Gracey N.; Alpar, E.Kaya
PUB. DATE
October 1999
SOURCE
Clinical & Investigative Medicine;Oct99, Vol. 22 Issue 5, p180
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Objective: Myosin heavy chain (MHC) fragment is part of a structural or force-bearing protein expressed in the thick filament of muscle fibres. Since MHC fragment is a contractile protein, an increase in plasma MHC concentrations after muscle injury indicates degradation of the contractile apparatus. This study was conducted to determine whether MHC concentrations could be a tool in the assessment of tissue damage in patients with myoskeletal injuries. Design: Prospective, controlled study. Setting: AUK University National Health Service Centre. Patients: Thirty-eight orthopedic patients, of whom 14 received surgical treatments within the 2-day study period. Patients were compared with 16 nonorthopedic control subjects. Outcome measures: Serum levels of MHC, creatine kinase, cardiac troponin I (cTnI), and myoglobin were measured at the time of admission and 24 hours later. Data from patients undergoing surgical repairs were obtained 24 hours after surgery. A competitive radioimmunoassay for Beta-type MHC was used. Results: Plasma MHC concentration was higher in the patients than in the controls. The peak levels were observed 24 hours after injury or surgery (p < 0.05). cTnI concentrations were consistently below the assay detection limit of 0.3 Mug/L, thus excluding protein release from the heart muscle (cardiac Beta-type MHC). Creatine kinase and myoglobin concentrations were significantly higher on admission in the non-surgical patients than in the surgically treated cases. Conclusions: Serum MHC levels could be a useful supplementary retrospective, prognostic or diagnostic tool in the study of myoskeletal disturbances involving muscle injury or bone fractures that result in membrane leakage of myoskeletal cells.
ACCESSION #
2502076

 

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