Identification of human hepatocellular carcinoma-related proteins by proteomic approaches

Ming-Hui Yang; Yu-Chang Tyan; Shiang-Bin Jong; Ying-Fong Huang; Pao-Chi Liao; Ming-Chen Wang
June 2007
Analytical & Bioanalytical Chemistry;Jun2007, Vol. 388 Issue 3, p637
Academic Journal
Hepatocellular carcinoma (HCC) is the most common malignant liver tumor. Analysis of human serum from HCC patients using two-dimensional gel electrophoresis (2DE) combined with nano-high-performance liquid chromatography electrospray ionization tandem mass spectrometry (nano-HPLC–ESI-MS/MS) identified fourteen different proteins differentially expressed between HCC patients and the control group. Twelve proteins were up-regulated and two down-regulated. By using nano-HPLC–MS/MS system to analyze proteome in human serum, 317 proteins were identified, twenty-nine of which to high confidence levels (protein matched at last two unique peptide sequences). Of these twenty-nine proteins, six were present only in HCC patients and may serve as biomarkers for HCC.


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