Determination of lead in blood by laser ablation ICP-TOF-MS analysis of blood spotted and dried on filter paper: a feasibility study

Cizdziel, James V.
June 2007
Analytical & Bioanalytical Chemistry;Jun2007, Vol. 388 Issue 3, p603
Academic Journal
Screening programs for pediatric lead (Pb) poisoning routinely collect blood by finger stick onto specially designed filter paper (FP) for subsequent laboratory testing. Here, laser ablation inductively coupled plasma time-of-flight mass spectrometry is evaluated as a novel method for the determination of Pb in blood spotted onto FP cards. The method requires no sample pretreatment and may be particularly suitable for rapidly screening a large number of samples. Signal intensity from the ICP-MS strongly correlated ( r 2 = 0.996) with Pb target values for five blood cards from a laboratory proficiency testing program. Better precision was obtained by replicate ablation line scans (<15% rsd) compared to a grid of 25 individual ablation spots (>30% rsd). Because <2% of the sample is consumed in the laser ablation analysis, selected samples can also be analyzed by traditional techniques or archived. Lead isotope ratios determined during the same total-Pb analysis appear to be effective for discerning contamination extraneous to the blood sample. Determining that an elevated result was due to contamination may negate the need for retesting with invasive pediatric venipuncture. Some elements monitored along with Pb exhibited signal profiles conducive to potential quantitation (Ca, V, Fe, Cu Zn), whereas others did not (Cr, Mn, Co, Ni, Cd, Hg, Tl, U). Copper, Ca, and V were inversely correlated with Pb ( r = −0.89, −0.90, and −0.92, respectively). Scanning electron microscope images revealed surface modality differences between FP spotted with fresh whole blood and reconstituted freeze-dried blood reference material. [Figure not available: see fulltext.]


Related Articles

  • Interaction of laser ablation plasma plume with grid screens. Anisimov, V.N.; Baranov, V. Yu. // Applied Physics Letters;11/13/1995, Vol. 67 Issue 20, p2922 

    Presents results of ablation plasma interaction with grid screens using emission spectroscopy and time-of-flight technique. Effect of velocity distribution transformation of the ion flow; Interaction of two plasma flows; Observance of three modes of velocity distribution function transformation.

  • Femtosecond laser ablation of gallium arsenide investigated with time-of-flight mass spectroscopy. Cavalleri, A.; Sokolowski-Tinten, K.; Bialkowski, J.; von der Linde, D. // Applied Physics Letters;5/11/1998, Vol. 72 Issue 19 

    We have investigated femtosecond laser-induced ablation of gallium arsenide using time-of-flight mass spectroscopy. At the ablation threshold, we estimated surface temperatures on the order of 3500 K. We observed a clear thresholdlike effect in the number of detected particles and with...

  • Study of ultraviolet-laser ablation products of several polymers using time-of-flight mass spectroscopy. Hansen, S. G. // Journal of Applied Physics;8/1/1989, Vol. 66 Issue 3, p1411 

    Presents information on a study which examined the material ejected into vacuum by pulsed laser ablation using time-of-flight mass spectroscopy (TOFMS). Ionization of permanent organic vapors; Laser ablation products from polymers; Advantages of employing two lasers and TOFMS in experiments.

  • An angle-resolved time-of-flight mass spectrometric study of pulsed laser ablated Ta2O5. Qin, Qi-Zong; Han, Zhen-Hui; Hai-Jun Dang // Journal of Applied Physics;6/1/1998 Part 1 of 2, Vol. 83 Issue 11, p6082 

    Focuses on a study which examined a pulsed laser ablation of a Ta2O5 target, with the usage of the angle-resolved time-of-flight quadrupole mass spectrometric method. Measurement of the ionic and neutral ablated species' mass and velocity distributions; Identification of the major ablated...

  • Reflection time-of-flight mass spectrometer for charge-transfer cross section measurements Kwong, Victor S.H.; Jiebing Wang // Review of Scientific Instruments;Oct97, Vol. 68 Issue 10, p3712 

    Describes a method that combines the production of ions by laser ablation with a reflection time-of-flight mass spectrometer for charge-transfer cross section measurements. Measurement of a specific charge-transfer channel of an ion-neutral pair at energies of about 0.1 keV/amu; Metastable...

  • Secondary electrons generated by fast neutral bombardment of the growing film during laser.... Brongersma, S.H.; Kools, J.C.S.; Baller, T.S.; Beijerinck, H.C.W.; Dieleman, J. // Applied Physics Letters;9/9/1991, Vol. 59 Issue 11, p1311 

    Examines the time-of-flight measurements on the films produced during excimer laser ablation of titanium nitride by fast neutral bombardment. Number of ions produced in the ablation process; Magnitude of the positive current due to secondary electrons.

  • Time-of-flight mass spectroscopic studies on the laser ablation process. Fukushima, Kensuke; Kanke, Yukio; Morishita, Tadataka // Journal of Applied Physics;12/1/1993, Vol. 74 Issue 11, p6948 

    Presents time-of-flight (TOF) mass spectroscopic studies on the laser ablation process. Description of the TOF mass spectrometer; Description and illustration of a typical TOF spectrum of the ionic species coming to the substrate; Function which explains the velocity distributions for the...

  • Time-of-flight and emission spectroscopy study of femtosecond laser ablation of titanium. Ye, Mengqi; Grigoropoulos, Costas P. // Journal of Applied Physics;5/1/2001, Vol. 89 Issue 9, p5183 

    Femtosecond laser ablation of titanium has been studied via time-of-flight (TOF) and emission spectroscopy measurement. Laser pulses of 80 fs full width at half maximum at λ=800 nm were delivered by a Ti:sapphire femtosecond laser system. A vacuum chamber with a base pressure of 10[sup -7]...

  • Velocity profiles of species ejected in ultraviolet laser ablation of several polymers examined by time-of-flight mass spectroscopy. Hansen, S. G. // Journal of Applied Physics;10/1/1989, Vol. 66 Issue 7, p3329 

    Discusses the velocity distributions of molecular species ejected by ultraviolet laser ablation of several polymers examined by time-of-flight mass spectroscopy. Experimental geometries used in the study; Typical examples showing the evolution of the mass spectra as a function of laser delay;...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics