Comparison of a novel ultra-performance liquid chromatographic method for determination of retinol and α-tocopherol in human serum with conventional HPLC using monolithic and particulate columns

Citová, I.; Havlíková, L.; Urbánek, L.; Solichová, D.; Nováková, L.; Solich, P.
June 2007
Analytical & Bioanalytical Chemistry;Jun2007, Vol. 388 Issue 3, p675
Academic Journal
Retinol and α-tocopherol are biologically active compounds often monitored in blood samples because of their evident importance in human metabolism. In this study a novel ultra-performance liquid chromatographic (UPLC) method used for determination of both vitamins in human serum has been compared with conventional HPLC with particulate and monolithic C18 columns. In UPLC a sub-two-micron particle-hybrid C18 stationary phase was used for separation, in contrast with a five-micron-particle packed column and a monolithic column with a highly porous structure. Methanol, at flow rates of 0.48, 1.5, and 2.5 mL min−1, respectively, was used as mobile phase for isocratic elution of the compounds in the three methods. Detection was performed at 325 nm and 290 nm, the absorption maxima of retinol and α-tocopherol, respectively. Analysis time, sensitivity, mobile-phase consumption, validation data, and cost were critically compared for these different chromatographic systems. Although cost and mobile-phase consumption seem to make UPLC the method of choice, use of the monolithic column resulted in almost the same separation and performance with a slightly shorter analysis time. These methods are alternatives and, in routine laboratory practice, more economical means of analysis of large numbers of biological samples than use of a traditional particulate column.


Related Articles

  • A metabolomic investigation of the effects of vitamin E supplementation in humans. Wong, Max; Lodge, John K. // Nutrition & Metabolism;2012, Vol. 9 Issue 1, p110 

    Background: Vitamin E is a nutrient with both antioxidant and non-antioxidant activities and has been shown to modulate the function of a number of cell types in vitro and in human studies. However studies have also shown vitamin E to have detrimental interactions and therefore it is important...

  • technical literature.  // Cosmetics & Toiletries;Sep2003, Vol. 118 Issue 9, p88 

    Discusses several studies related to cosmetics and skin care. Penetration of vitamin E acetate applied from cosmetic formulations to the stratum corneum of an in vitro model using quantification by tape stripping, UV spectroscopy, and high performance liquid chromatography; Determination of a...

  • Rapid Estimation of Tocopherol Content in Linseed and Sunflower Oils-Reactivity and Assay. Prevc, Tjaša; Levart, Alenka; Cigiæ, Irena Kralj; Salobir, Janez; Ulrih, Nataša Poklar; Cigiæ, Blaž // Molecules;Aug2015, Vol. 20 Issue 8, p14777 

    The reactivity of tocopherols with 2,2-diphenyl-1-picrylhydrazyl (DPPH) was studied in model systems in order to establish a method for quantifying vitamin E in plant oils. The method was optimized with respect to solvent composition of the assay medium, which has a large influence on the course...

  • Plasma Concentrations of Vitamin E, Vitamin A and �-Carotene in Healthy Men. Grzelinska, Z.; Gromadzinska, J.; Swiercz, R.; Wasowicz, W. // Polish Journal of Environmental Studies;2007, Vol. 16 Issue 2, p209 

    The aim of the study was to adapt the method for the determination of vitamins E, A, and �-carotene and to assay them quantitatively in plasma of municipal transport drivers. The study embraced 147 municipal transport male drivers, aged 23-58 years. The waters 2695 integrated analytical...

  • Determination of α -Tocopherol in Plasma by High Performance Liquid Chromatography with Fluorescence Detection and Stability of α -Tocopherol under Different Conditions. Rümelin, Andreas; Humbert, Till; Fauth, Ulrich // Drug Research / Arzneimittel-Forschung (Editio Cantor Verlag fur;Jul2004, Vol. 54 Issue 7, p376 

    In order to study the clinical effects of the antioxidant α-tocopherol (tocofersolan, CAS 30999-06-5) a simple, robust, sensitive and reliable high performance liquid chromatography (HPLC) method using fluorescence detection for the daily measurement of α-tocopherol concentration in plasma...

  • Alpha Tocopherol Content of Selected Catering Products. Mazarel, Adrian; Rotaru, Gabriela; Matea, Cristian; Bunea, Andreea; Bele, Constantin // Bulletin of the University of Agricultural Sciences & Veterinary;2010, Vol. 67 Issue 2, p303 

    Alpha tocopherol is recognized as inhibitor of lipid oxidation in biological systems and the intake of this vitamin is relevant to human health .Catering food products investigated in the current study have been chosen from two food groups: fish based food products and meat based food products....

  • Rapid Method for the Determination of Vitamins A and E in Foods Using Ultra-High-Performance Liquid Chromatography. You-Shin Shim; Ki-Jin Kim; Dongwon Seo; Ito, Masahito; Nakagawa, Hiroaki; Jaeho Ha // Journal of AOAC International;Mar/Apr2012, Vol. 95 Issue 2, p517 

    The article discusses a study which validated a rapid and novel ultra-high performance liquid chromatography (u-HPLC) method for the determination of vitamins A and E in foods in terms of its precision, accuracy and linearity. The study showed that the recovery of vitamin A or retinol was more...

  • Rapid and Simple Determination of Vitamin A and Vitamin E in Human Plasma by Column-Switching High-Performance Liquid Chromatography. Sadílek, Petr; Šatínský, Dalibor; Ot'apka, Martin; Sladkovský, Radek; Solich, Petr // Current Analytical Chemistry;Oct2009, Vol. 5 Issue 4, p311 

    In this contribution, the rapid and simple determination of vitamins A and E, and vitamin E acetate as internal standard in human plasma by high-performance liquid chromatography with a column-switching technique was proposed. Restricted Access Material - RAM column 25 x 4 mm containing 25 µm...

  • The Effect of Physical Refining on Palm Vitamin E (Tocopherol, Tocotrienol and Tocomonoenol). Chiew Wei Puah; Yuen May Choo; Ah Ngan Ma; Cheng Hock Chuah // American Journal of Applied Sciences;2007, Vol. 4 Issue 6, p374 

    This study reports the effect of physical refining on palm vitamin E including α-tocopherol, α-, γ- and ƍ-tocotrienols as well as α-tocomonoenol. A method using HPLC with fluorescence detector using normal phase silica column is described. An isocratic elution with...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics