TITLE

The Arxula adeninivorans ATAL gene encoding transaldolase-gene characterization and biotechnological exploitation

AUTHOR(S)
Fiki, Ayman El; Metabteb, Gamal El; Bellebna, Carmen; Wartmann, Thomas; Bode, Rüdiger; Gellissen, Gerd; Kunze, Gotthard
PUB. DATE
April 2007
SOURCE
Applied Microbiology & Biotechnology;Apr2007, Vol. 74 Issue 6, p1292
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The yeast Arxula adeninivorans provides an attractive expression platform and can be exploited as gene source for biotechnologically interesting proteins. In the following study, a striking example for the combination of both aspects is presented. The transaldolase-encoding A. adeninivorans ATAL gene, including its promoter and terminator elements, was isolated and characterized. The gene includes a coding sequence of 963 bp encoding a putative 321 amino acid protein of 35.0 kDa. The enzyme characteristics analyzed from isolates of native strains and recombinant strains overexpressing the ATAL gene revealed a molecular mass of ca. 140 kDa corresponding to a tetrameric structure, a pH optimum of ca. 5.5, and a temperature optimum of 20°C. The preferred substrates for the enzyme include d-erythrose-4-phosphate and d-fructose-6-phosphate, whereas d-glyceraldehyde is not converted. The ATAL expression level under salt-free conditions was observed to increase in media supplemented with 5% NaCl rendering the ATAL promoter attractive for moderate heterologous gene expression under high-salt conditions. Its suitability was assessed for the expression of a human serum albumin ( HSA) reporter gene.
ACCESSION #
24939528

 

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