TITLE

Distinct levels in the nanoscale organization of DNA-histone complex revealed by its mechanical unfolding

AUTHOR(S)
Soni, G. V.; Brar, Loveleen; Hameed, Feroz M.; Raychaudhuri, A. K.; Shivashankar, G. V.
PUB. DATE
April 2007
SOURCE
Applied Physics Letters;4/16/2007, Vol. 90 Issue 16, p163904
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Mechanical unfolding of nanoscale DNA-histone complex, using an atomic force microscope, shows a stepwise disassembly of histones from the nucleosome. A quantitative analysis of the rupture jump statistics and the length released per jump reveals insights into the possible histone contacts within the octamer complex. The measured ruptures correlate with the breakage of multiple contacts that stabilize the histone octamer. These results provide a mechanistic basis by which stepwise disassembly of histone proteins may result from an external force exerted by the adenosinetriphosphate (ATP) dependent chromatin remodeling machines to access regulatory sites on DNA.
ACCESSION #
24890678

 

Related Articles

  • Dynamics of ATP-dependent chromatin assembly by ACF. Fyodorov, Dmitry V.; Kadonaga, James T. // Nature;8/22/2002, Vol. 418 Issue 6900, p897 

    Shows that adenosine triphosphate (ATP)-dependent chromatin-assembly factor (ACF) becomes committed to the DNA template upon initiation of chromatin assembly. Deposition of histones into periodic nucleosome arrays; Dynamics of chromatin assembly by ACF; DNA supercoiling analysis; Use of...

  • Chromatin remodeling complexes: ATP-dependent machines in action. Johnson, Cotteka N.; Adkins, Nicholas L.; Georgel, Philippe // Biochemistry & Cell Biology;Aug2005, Vol. 83 Issue 4, p405 

    Since the initial characterization of chromatin remodeling as an ATP-dependent process, many studies have given us insight into how nucleosome-remodeling complexes can affect various nuclear functions. However, the multistep DNA-histone remodeling process has not been completely elucidated....

  • Nucleosome sliding mechanisms: new twists in a looped history. Mueller-Planitz, Felix; Klinker, Henrike; Becker, Peter B // Nature Structural & Molecular Biology;Sep2013, Vol. 20 Issue 9, p1026 

    Nucleosomes, the basic organizational units of chromatin, package and regulate eukaryotic genomes. ATP-dependent nucleosome-remodeling factors endow chromatin with structural flexibility by promoting assembly or disruption of nucleosomes and the exchange of histone variants. Furthermore, most...

  • Proteasome system of protein degradation and processing. Sorokin, A. V.; Kim, E. R.; Ovchinnikov, L. P. // Biochemistry (00062979);Dec2009, Vol. 74 Issue 13, p1411 

    In eukaryotic cells, degradation of most intracellular proteins is realized by proteasomes. The substrates for proteolysis are selected by the fact that the gate to the proteolytic chamber of the proteasome is usually closed, and only proteins carrying a special “label” can get into...

  • ATP-Dependent Chromatin Structural Modulation by Multiprotein Complex Including HMGB1. Yamada, Masamichi; Ueda, Tetsuya; Sato, Koichi; Yoshida, Michiteru // Journal of Biochemistry;Jan2004, Vol. 135 Issue 1, p149 

    High mobility group box protein 1, HMGB1, is a major nonhistone chromatin component in higher eukaryotic cells. HMGB1 is thought to be involved in the processes of global nuclear events such as transcription, recombination and repair, but the mechanism of these processes is unclear. Here, we...

  • The Emerging Roles of ATP-Dependent Chromatin Remodeling Enzymes in Nucleotide Excision Repair. Czaja, Wioletta; Mao, Peng; Smerdon, Michael J. // International Journal of Molecular Sciences;Sep2012, Vol. 13 Issue 9, p11954 

    DNA repair in eukaryotic cells takes place in the context of chromatin, where DNA, including damaged DNA, is tightly packed into nucleosomes and higher order chromatin structures. Chromatin intrinsically restricts accessibility of DNA repair proteins to the damaged DNA and impacts upon the...

  • Five repair pathways in one context: chromatin modification during DNA repair. Ataian, Yeganeh; Krebs, Jocelyn E. // Biochemistry & Cell Biology;Aug2006, Vol. 84 Issue 4, p490 

    The eukaryotic cell is faced with more than 10 000 various kinds of DNA lesions per day. Failure to repair such lesions can lead to mutations, genomic instability, or cell death. Therefore, cells have developed 5 major repair pathways in which different kinds of DNA damage can be detected and...

  • Chromatin remodeling enzymes: taming the machines. Peterson, Craig L. // EMBO Reports;Apr2002, Vol. 3 Issue 4, p319 

    Members of the ATP-dependent family of chromatin remodeling enzymes play key roles in the regulation of transcription, development, DNA repair and cell cycle. Each of these enzymes are multi-subunit assemblies that hydrolyze thousands of molecules of ATP in order to change nucleosome positions,...

  • ATP-Dependent Chromatin Remodeling Factors and Their Roles in Affecting Nucleosome Fiber Composition. Piatti, Paolo; Zeilner, Anette; Lusser, Alexandra // International Journal of Molecular Sciences;Oct2011, Vol. 12 Issue 10, p6544 

    ATP-dependent chromatin remodeling factors of the SNF2 family are key components of the cellular machineries that shape and regulate chromatin structure and function. Members of this group of proteins have broad and heterogeneous functions ranging from controlling gene activity, facilitating DNA...

Share

Read the Article

Courtesy of NEW JERSEY STATE LIBRARY

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics