šiugždinienė, Rita; Dobilas, Justinas Antanas
December 2006
Veterinarija ir Zootechnika;2006, Vol. 36 Issue 58, p75
Academic Journal
Adjuvantų parinkimas vakcinoms gaminti labai aktualus atliekant kiaulių raudonligės infekcijos specifinę profilaktiką. Atlikome tyrimus su aliejiniais adjuvantais, kurie yra mažiau reaktogeniški, kenksmingi, nei kiti vakcinų gamybai naudojami adjuvantai (Freundo adjuvantas, saponinai), ir palyginome jų imuninio atsako stimuliacijos poveikį su aliuminio hidroksido adjuvantu komercinėms vakcinoms gaminti. Kliniškai vakciną įvertinome vakcinuodami paršelius. Paruošėme kontrolinį vakcinos bandinį su „Emulsigen 75‟ ir „Emulsigen‟ (MVP Laboratories Inc., USA) adjuvantais. Po vakcinacijos visų grupių paršeliai buvo užkrėsti 100 LD 50 dozėmis virulentiška Rb suspensija. Tyrimų metu bandomiesiems gyvuliams iš ausies venos imtas kraujas ir klasikine agliutinacijos reakcija nustatomi antikūnų titrai. Veislinės paršavedės nuolat vakcinuojamos nuo raudonligės. Tik gimę paršeliai įgyja kolostralinį imunitetą, todėl prieš bandymą antikūnų titrus nustatėme visose tiriamosiose grupėse. Aukščiausi antikūnų titrai paršelių kraujo serume buvo praėjus 28 paroms po antros vakcinacijos. Antikūnų titrai paršelių kraujo serume mažėjo iki 7 paros po užkrėtimo. Mūsų tyrimų duomenimis, monovalentinei vakcinai nuo kiaulių raudonligės gaminti optimaliausias adjuvantas - 2 proc. koncentracijos ‘Emulsigen’. Antikūnų titrų tyrimai parodė, kad LVA VI sukurtos ir paruoštos inaktyvuotos vakcinos su adjuvantu ‘Emulsigen’ poveikis imunizuojant paršelius atitinka komercinės vakcinos imunologinius rodiklius.


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