Strategies for laboratory HIV testing: an examination of alternative approaches not requiring Western blot

Sato, P. A.; Maskill, W. J.; Tamashiro, H.; Heymann, D. L.
January 1994
Bulletin of the World Health Organization;1994, Vol. 72 Issue 1, p129
Academic Journal
Advances in laboratory tests for antibodies to human immunodeficiency virus (HIV) have permitted the development of alternative HIV testing strategies that do not require use of the Western blot approach. Three strategies are proposed. In strategy I, sera are tested for HIV antibody using an enzyme-linked immunosorbent assay (ELISA)/rapid/simple (ERS) test; in strategy II, sera reactive in an initial ERS test are retested using a second ERS test; strategy III involves retesting with a third ERS test all sera reactive in two previous ERS tests. Where the objective is identification of asymptomatic HIV-infected individuals, strategy III is proposed where HIV prevalences in the study population are ≤ 10%, and strategy II at prevalences >10%. Strategy II is recommended where the diagnosis of HIV-related disease requires HIV testing. For serosurveillance, strategy II is recommended if the prevalence is ≤10%, and strategy I if the prevalences are >10%. Use of strategy I is recommended for transfusion and transplantation safety, at any prevalence. Lower-cost laboratory HIV testing will permit such testing to become more widely available.


Related Articles

  • In Reply. Hull, Harry F.; Keller, Nick M.; Wilson, Jane; Bettinger, Carl J.; Gallagher, Margaret M.; Mertz, Gregory J. // JAMA: Journal of the American Medical Association;5/5/89, Vol. 261 Issue 17, p2502 

    Responds to a critique of the authors' article 'Comparison of HIV-Antibody Testing in an STD Clinic, published in a 1988 issue of the Journal of the American Medical Association. Ethical issues surrounding the blinded study conducted on persons who refused anonymous HIV testing; Distinctions...

  • North Carolina.  // Advocate;3/21/95, Issue 677, p12 

    Reports on the decision of the North Carolina health services commission to end anonymous HIV antibody testing in June 15, 1995.

  • Oral HIV testing in San Mateo County. Laird, Cynthia // Bay Area Reporter;4/21/2005, Vol. 35 Issue 16, p4 

    Reports that the Asian and Pacific Islander Wellness Center is offering free, confidential and anonymous standard oral HIV antibody testing in San Mateo County, California.

  • HIV counseling and testing--United States, 1993.  // MMWR: Morbidity & Mortality Weekly Report;3/10/1995, Vol. 44 Issue 9, p169 

    Presents an analysis of data sources concerning human immunodeficiency virus (HIV) counseling and testing in the United States in 1993. HIV-antibody tests; Reporting area; Health care visits; Limitations of report findings; Measures to prevent the spread of HIV.

  • Agenda.  // Advocate;4/18/95, Issue 679, p10 

    Reports that about 33 percent of people in the United States tested for HIV antibodies were tested at a publicly funded clinic.

  • Update: HIV counseling and testing using rapid tests--United States, 1995.  // MMWR: Morbidity & Mortality Weekly Report;3/27/1998, Vol. 47 Issue 11, p211 

    Presents information on the testing for antibodies to human immunodeficiency virus (HIV) in the United States, and funded counseling and testing (CT) programs conducted. Reference to the design of a decision model; Mention of the rapid test (Single Use Diagnostic System SUDS); Detailed...

  • Detection of Acute Infections during HIV Testing in North Carolina. Pilcher, Christopher D.; Fiscus, Susan A.; Nguyen, Trang Q.; Foust, Evelyn; Wolf, Leslie; Williams, Del; Ashby, Rhonda; O'Dowd, Judy Owen; McPherson, J. Todd; Stalzer, Brandt; Hightow, Lisa; Miller, William C.; Eron, Joseph J.; Cohen, Myron S.; Leone, Peter A. // New England Journal of Medicine;5/5/2005, Vol. 352 Issue 18, p1873 

    Background: North Carolina has added nucleic acid amplification testing for the human immunodeficiency virus (HIV) to standard HIV antibody tests to detect persons with acute HIV infection who are viremic but antibody-negative. Methods: To determine the effect of nucleic acid amplification...

  • Detection of Acute HIV Infection in Two Evaluations of a New HIV Diagnostic Testing Algorithm -- United States, 2011-2013. Geren, Kara; Moore, Eric; Tomlinson, Cheri; Hobohm, Dan; Gardner, Ann; Reardon-Maynard, Deborah; Gay, Cindy; Hightow-Weidman, Lisa B.; Pandori, Mark W.; Moss, Nicholas; Westheimer, Emily; Tsoi, Benjamin; Branson, Bernard M.; Peters, Philip J. // MMWR: Morbidity & Mortality Weekly Report;6/21/2013, Vol. 62 Issue 24, p489 

    The article discusses the U.S. Centers for Disease Control and Prevention's (CDC) two evaluations of an HIV diagnostic algorithm. It addresses how the problem of false-negative results early in the course of HIV infection can be resolved by testing for HIV RNA after a reactive immunoassay (AI)...

  • Interpopulation variation in HIV testing promptness may introduce bias in HIV incidence estimates using the serologic testing algorithm for recent HIV seroconversion. White, Edward; Goldbaum, Gary; Goodreau, Steven; Lumley, Thomas; Hawes, Stephen E. // Sexually Transmitted Infections;Aug2010, Vol. 86 Issue 4, p4 

    Objectives: The serologic testing algorithm for recent HIV seroconversion (STARHS) calculates incidence using the proportion of testers who produce a level of HIV antibody high enough to be detected by ELISA but low enough to suggest recent infection. The validity of STARHS relies...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics