In Vivo Efficacy of Antimicrobial-Coated Devices

Darouiche, Rabih O.; Mansouri, Mohammad D.; Zakarevicz, Devin; AlSharif, Atef; Landon, Glenn C.
April 2007
Journal of Bone & Joint Surgery, American Volume;Apr2007, Vol. 89-A Issue 4, p792
Academic Journal
Background: Since device colonization is a prelude to infection, an antimicrobial-coated device that reduces bacterial colonization can potentially protect against infection. The objective of this animal study was to assess the efficacy of a coating with minocycline and rifampin to prevent colonization of a grit-blasted titanium implant and subsequent osteomyelitis. Methods: Twenty-five rabbits underwent implantation of a titanium-alloy pin, either coated with minocycline and rifampin (thirteen rabbits) or uncoated (twelve rabbits), into the right femoral medullary canal. The implanted devices were inoculated with 500 CFU (colony-forming units) of Staphylococcus aureus prior to wound closure. The rabbits were killed one week later, and the removed device, femoral bone, a specimen obtained by swabbing the track surrounding the device, and blood were cultured. The rates of device colonization, osteomyelitis, and device-related osteomyelitis were compared between the two groups of rabbits. Results: The antimicrobial-coated devices had a significantly lower rate of colonization than the uncoated devices (five of thirteen compared with twelve of twelve, p = 0.0016) and were associated with significantly lower rates of Osteomyelitis (six of thirteen compared with twelve of twelve, p = 0.005) and device-related osteomyelitis (five of thirteen compared with twelve of twelve, p = 0.0016). Bacteremia did not develop in any rabbit. Conclusions: Orthopaedic devices coated with minocycline and rifampin significantly protected against device colonization and infection due to Staphylococcus aureus in this in vivo rabbit model. Clinical Relevance: It is possible that orthopaedic devices coated with this unique combination of antimicrobial agents may protect against the development of clinical infection in humans.


Related Articles

  • Capacitive Coupling Reduces Instrumentation-related Infection in Rabbit Spines: A Pilot Study. Gilotra, Mohit; Griffith, Cullen; Schiavone, Jason; Nimmagadda, Naren; Noveau, Jenna; Ludwig, Steven // Clinical Orthopaedics & Related Research;Jun2012, Vol. 470 Issue 6, p1646 

    Background: Postoperative spine infections cause considerable morbidity. Patients are subjected to long-term antibiotic regimens and may require further surgery. Delivery of electric current through instrumentation can detach biofilm, allowing better antibiotic penetration and assisting in...

  • Efficacy of antimicrobial polymer coatings in an animal model of bacterial infection associated with foreign body implants. Hart, Emily; Azzopardi, Kristy; Heng Taing; Graichen, Florian; Jeffery, Justine; Mayadunne, Roshan; Wickramaratna, Malsha; O'Shea, Mike; Nijagal, Brunda; Watkinson, Rebecca; O'Leary, Stephen; Finnin, Barrie; Tait, Russell; Robins-Browne, Roy // Journal of Antimicrobial Chemotherapy (JAC);May2010, Vol. 65 Issue 5, p974 

    Objectives: To assess support discs, comprising polyethylene terephthalate (PET), coated with different polymer/levofloxacin combinations for antimicrobial activity in an animal model of infection, in order to explore the use of specific polymer coatings incorporating levofloxacin as a means of...

  • Better Prophylaxis Against Surgical Site Infection with Local as Well as Systemic Antibiotics: An in Vivo Study. Cavanaugh, Daniel L.; Berry, John; Yarboro, Seth R.; Dabners, Laurence E. // Journal of Bone & Joint Surgery, American Volume;Aug2009, Vol. 91-A Issue 8, p1907 

    Background: Prophylactic systemic antibiotics significantly lower the risk of postoperative infection, and injection of antibiotics directly into the wound cavity has been found to be even more effective. In this study, we investigated the efficacy of direct injection of antibiotics into a wound...

  • The Mutant Selection Window in Rabbits Infected with Staphylococcus aureus. Junchang Cui; Youning Liu; Rui Wang; Weihang Tong; Drlica, Karl; Xilin Zhao // Journal of Infectious Diseases;12/1/2006, Vol. 194 Issue 11, p1601 

    Background. The mutant selection window hypothesis, originally based on agar plate assays, may lead to new antimicrobial dosing strategies that severely restrict the acquisition of resistance. However, it has not been directly tested in an animal model of infection. Methods. Local infection with...

  • Study of Bacteria Isolated from Orthopedic Implant Infections and their Antimicrobial Susceptibility Pattern. Khosravi, A. D.; Ahmadi, F.; Salmanzadeh, S.; Dashtbozorg, A.; Montazeri, E. Abasi // Research Journal of Microbiology;2009, Vol. 4 Issue 4, p158 

    The aim of the present study was, to determine the bacteriology of orthopedic implant infections and susceptibilities of isolated bacteria to the commonly used antimicrobial agents. One hundred and sixty five patients were investigated for early or late postoperative infections of orthopedic...

  • Dose-dependent relationship found for CA-MRSA, antibacterials.  // Infectious Disease News;Sep2011, Vol. 24 Issue 9, p14 

    The article discusses a research study in Great Britain which found a relationship between exposure to antimicrobials and increased risk for community-associated methicillin resistant Staphyloccocus aureus (CA-MRSA).

  • Are Bone and Serum Cefazolin Concentrations Adequate for Antimicrobial Prophylaxis? Yamada, Koji; Matsumoto, Kunihiro; Tokimura, Fumiaki; Okazaki, Hiroshi; Tanaka, Sakae // Clinical Orthopaedics & Related Research;Dec2011, Vol. 469 Issue 12, p3486 

    Background: Antimicrobial prophylaxis is considered beneficial for preventing surgical-site infections in clean orthopaedic surgery. However, whether tissue concentrations of cefazolin achieve the minimum inhibitory concentration for the targeted contaminants have yet to be clarified....

  • Radiological signs of osteitis around extramedullary metal implants : A radiographic-microbiological correlative analysis in rabbit tibiae after local inoculation of Staphylococcus aureus. Kraft, C. N.; Schlegel, U.; Pfluger, Dominik; Eijer, Hendrik; Textor, Jochen; Hansis, Martin; Arens, Stephan // Archives of Orthopaedic & Trauma Surgery;Jun2001, Vol. 121 Issue 6, p338 

    Radiographic changes in the early stages of osteomyelitis may be subtle and, especially after plate osteosynthesis, frequently missed. A previously described experimental model of local bacterial infection was used in an attempt to determine the reliability of specific changes on conventional...

  • Staphylococcus aureus Screening and Decolonization in Orthopaedic Surgery and Reduction of Surgical Site Infections. Chen, Antonia; Wessel, Charles; Rao, Nalini // Clinical Orthopaedics & Related Research;Jul2013, Vol. 471 Issue 7, p2383 

    Background: Staphylococcus aureus is the most common organism responsible for orthopaedic surgical site infections (SSIs). Patients who are carriers for methicillin-sensitive S. aureus or methicillin-resistant S. aureus (MRSA) have a higher likelihood of having invasive S. aureus infections....


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics