TITLE

Effect of chronic Giardia lamblia infection on epithelial transport and barrier function in human duodenum

AUTHOR(S)
Troeger, Hanno; Epple, Hans-Joerg; Schneider, Thomas; Wahnschaffe, Uirich; Ulirich, Reiner; Burchard, Gerd-Dieter; Jelinek, Tomas; Zeilz, Martin; Fromm, Michael; Schulzke, Joerg-Dieter
PUB. DATE
March 2007
SOURCE
Gut;Mar2007, Vol. 56 Issue 3, p328
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Giardia Iamblia causes infection of the small intestine, which leads to malabsorption and chronic diarrhoea. Aim: To characterise the inherent pathomechanisms of G Iamblia infection. Methods: Duodenal biopsy specimens from 13 patients with chronic giardiasis and from controls were obtained endoscopically. Short-circuit current (Isc) and mannitol fluxes were measured in miniaturised Ussing chambers. Epithelial and subepithelial resistances were determined by impedance spectroscopy. Mucosal morphometry was performed and tight junction proteins were characterised by immunoblotting. Apoptotic ratio was determined by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labelling staining. Results: In giardiasis, mucosal surface area per unit serosa area was decreased to 75% (3%) of control, as a result of which epithelial resistance should increase. Instead, epithelial resistance of giardiasis biopsy specimens was decreased (19 (2) vs 25 (2) Ω cm2 p<0.05) whereas mannitol flux was not significantly altered (140 (27) vs 105 (16) nmol/h/cm2). As structural correlate, reduced claudin 1 expression and increased epithelial apoptosis were detected. Furthermore, basal lsc increased from 191 (20) in control to 261 (12) μA/h/cm2 in giardiasis. The bumetanide-sensitive portion of lsc in giardiasis was also increased (51 (5) vs 20 (9) μA/h/cm2 in control; p<0.05). Finally, phlorizin-sensitive Na+-glucose symport was reduced in patients with giardiasis (121(9) vs 83 (14) μA/h/cm2). Conclusions: G Iamblia infection causes epithelial barrier dysfunction owing to down regulation of the tight junction protein claudin 1 and increased epithelial apoptoses. Na+-dependent D-glucose absorption is impaired and active electrogenic anion secretion is activated. Thus, the mechanisms of diarrhoea in human chronic giardiasis comprise leak flux, malabsorptive and secretory components.
ACCESSION #
24528556

 

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