TITLE

A new amperometric biosensor for hydrogen peroxide determination based on HRP-nanogold-PTH-nanogold-modified carbon paste electrodes

AUTHOR(S)
Fuchang Wang; Ruo Yuan; Yaqin Chai
PUB. DATE
May 2007
SOURCE
European Food Research & Technology;May2007, Vol. 225 Issue 1, p95
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Abstract  A new strategy for immobilization of horseradish peroxidase (HRP) has been developed by self-assembling gold nanoparticles to multiporous polythionine (PTH) film modified carbon paste interface. A thionine film was initially electropolymerized onto carbon paste interface in a mildly acidic thionine solution at a bias voltage of −1.0 to 1.5 V. This process is accompanied by the hydrogen evolution reaction, and the released hydrogen gas made the PTH film with multiporous structure. The multiporous PTH film provided a biocompatible microenvironment for gold nanoparticles and enzyme molecules, and greatly amplified the coverage of HRP molecules on the electrode surface. Voltammetric and time-based amperometric techniques were employed to characterize the properties of the derived biosensor. The performance and factors influencing the performance of the biosensor were also proposed. The immobilized HRP displayed a catalytic property to the reduction of H2O2. The H2O2biosensor achieved 95% of the steady-state current within 2 s, and exhibited a linear range of 9.6?10−6to 1.2?10−3 M H2O2with a detection limit of 7.5?10−7 M (S/N= 3). Furthermore, the biosensor remained at about 90% of its original sensitivity after 2 weeks’ storage.
ACCESSION #
24517852

 

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