Patterns of Host Genome-Wide Gene Transcript Abundance in the Peripheral Blood of Patients with Acute Dengue Hemorrhagic Fever

Simmons, Cameron P.; Popper, Stephen; Dolocek, Christiane; Tran Nguyen Bich Chau; Griffiths, Michael; Nguyen Thi Phuong Dung; Truong Hoang Long; Dang Minh Hoang; Nguyen Vinh Chau; Le Thi Thu Thao; Tran Tinh Hien; Relman, David A.; Farrar, Jeremy
April 2007
Journal of Infectious Diseases;4/15/2007, Vol. 195 Issue 8, p1097
Academic Journal
Responses by peripheral blood leukocytes may contribute to the pathogenesis of dengue hemorrhagic fever (DHF). We used DNA microarrays to reveal transcriptional patterns in the blood of 14 adults with DHF. Acute DHF was defined by an abundance of transcripts from cell cycle- and endoplasmic reticulum (ER)-related genes, suggesting a proliferative response accompanied by ER stress. Transcript-abundance levels for immunoresponse- associated genes, including cell surface markers, immunoglobulin, and innate response elements, were also elevated. Twenty-four genes were identified for which transcript abundance distinguished patients with dengue shock syndrome (DSS) from those without DSS. All the gene transcripts associated with DSS, many of which are induced by type I interferons, were less abundant in patients with DSS than in those without DSS. To our knowledge, these data provide the first snapshot of gene-expression patterns in peripheral blood during acute dengue and suggest that DSS is associated with attenuation of selected aspects of the innate host response.


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