TITLE

Inhibitory analysis of the effect of polycyclic aromatic hydrocarbons on the activity of chitinase by means of liquid chromatography-mass spectrometry of chitin oligosaccharides

AUTHOR(S)
Ooki, Yoriko; Kumemura, Momoko; Itoh, Masayoshi; Korenaga, Takashi
PUB. DATE
April 2007
SOURCE
Analytical & Bioanalytical Chemistry;Apr2007, Vol. 387 Issue 8, p2641
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The analytical method of determining enzyme activity by liquid chromatography-mass spectrometry (LC/MS) was developed and applied for investigation of the effect of polycyclic aromatic hydrocarbons (PAHs) on the enzyme activity of chitinase. The measurement of chitinase activity by LC/MS is useful in order to use the nonderivatized substrate, which can show in vivo chitinase activity. Substrate consumption and product formation were monitored in order to determine chitinase activity. It was shown that, for the first time, in vitro addition of PAHs inhibited the activity of chitinase in a noncompetitive manner. The IC50 value of benzo[ a]pyrene was 1.4 μM, and PAHs containing four or more aromatic rings showed the same or higher inhibitory effect, whereas PAHs with a lower number of aromatic rings showed lower inhibition of the chitinase activity than benzo[ a]pyrene.
ACCESSION #
24441174

 

Related Articles

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics