Malignant B Cells From Patients With Primary Central Nervous System Lymphoma Express Stromal Cell-Derived Factor-1

Katherine Falkenhagen; Sarah Coupland; Timothy Chipps; Rita Braziel
April 2007
American Journal of Clinical Pathology;Apr2007, Vol. 127 Issue 4, p633
Academic Journal
Although the pathogenesis of primary central nervous system lymphoma (PCNSL) remains unclear, it is hypothesized that specific chemokine-chemokine receptor interactions may attract malignant B lymphocytes into the CNS. Formalin-fixed, paraffin-embedded brain biopsy specimens from 40 patients with PCNSL were immunostained by an indirect immunohistochemical method incorporating antigen retrieval to detect the presence of B-cell chemokines, stromal cell-derived factor-1 (SDF-1; CXCL12) and macrophage inflammatory protein-3α (MIP-3α, CCL20), and the SDF-1 receptor, CXCR4. To assist in phenotyping of SDF-1 + cells, specimens were also stained for CD20 (B cells). Positive staining for SDF-1 was identified in all PCNSL cases and in tonsil. In biopsy specimens, SDF-1 expression was localized to resident brain cells and, in 80% of specimens, CD20+ malignant lymphocytes. Tumor cells also stained positively for CXCR4. In contrast, although expression ofMIP-3α was detected in tonsil, no expression of this chemokine could be demonstrated in PCNSL biopsy specimens. Our observations raise the possibility of targeting the SDF-1-CXCR4 signaling pathway as a potential treatment for PCNSL.


Related Articles

  • Look to cells, not site, to diagnose B-cell lymphoma. Hill, Suzerre // Dermatology Times;Nov2004, Vol. 25 Issue 11, p39 

    The article reports the cutaneous lymphoma classification proposed by the World Health Organization and the European Organization for Research and Treatment of Cancer reflects a welcome consensus of the two organizations' existing classifications of cutaneous B-cell lymphomas. the newly proposed...

  • Primary lymphoblastic B-cell lymphoma of the stomach: A case report. Miao-Xia He; Ming-Hua Zhu; Wei-Qiang Liu; Li-Li Wu; Xiong-Zeng Zhu; Al Mofleh, Ibrahim A. // World Journal of Gastroenterology;5/21/2008, Vol. 14 Issue 19, p3101 

    Primary stomach lymphoblastic B-cell lymphoma (B-LBL) is a rare tumor. We describe a primary stomach B-LBL in a 38 years old female who presented with nonspecific complaints of fatigue and vomiting for 2 mo. Gastrofiberscopy revealed a large gastric ulcer, which was successfully resected....

  • Follicular lymphoma. Pantanowitz, Liron; Thompson, Lester D. R. // ENT: Ear, Nose & Throat Journal;Oct2006, Vol. 85 Issue 10, p636 

    The article presents information on follicular lymphoma. Follicular lymphoma is defined as a neoplasm of follicle center B lymphocytes that has at least a partially follicular growth pattern. It is a low-grade lymphoma, and its course is indolent. The histologic variants of follicular lymphoma...

  • B cell lymphoma causing splenomegaly in a Sundevall's jird (Meriones crassus subspecies perpallidus). Wenzlow, N.; Stanley, R.L.; Patterson-Kane, J.C. // Veterinary Record: Journal of the British Veterinary Association;4/5/2008, Vol. 162 Issue 14, p455 

    The article describes a case of disseminated B cell lymphoma causing splenomegaly in a three-year-old female Sundevall's jird. The pet jird was presented with a history of mild lethargy and depression, two days before sudden death. The result of microscopic examination showed sheets of...

  • Radioimmunotherapy may yield benefits in patients with B-cell lymphoma. Raible, Eric // Hem/Onc Today;11/25/2008, Vol. 9 Issue 21, p19 

    This article focuses on two studies which demonstrated the potential therapeutic role of radioimmunotherapy in the treatment of patients with B-cell lymphomas. An overview of the study population, methodology and key findings is provided. The limitations and implications of radioimmunotherapy...

  • The role of chemokines in B cell chronic lymphocytic leukaemia: pathophysiological aspects and clinical impact. Schröttner, Percy; Leick, Marion; Burger, Meike // Annals of Hematology;May2010, Vol. 89 Issue 5, p437 

    Chemokines are centrally involved in leukocyte migration, homing and haematopoiesis. Besides these physiological aspects, their role in pathological processes especially with respect to solid tumour and haematological neoplasias is well established. In this context, the focus was set here on...

  • Diagnostic and prognostic utility of PD-1 in B cell lymphomas. Muenst, S.; Hoeller, S.; Willi, N.; Dirnhofera, S.; Tzankov, A. // Disease Markers;2010, Vol. 29 Issue 1, p47 

    Aims: Programmed death-1 (PD-1) is expressed by germinal center-associated helper T-cells and acts as a negative regulator of the immune system. PD-1 is encountered on tumor cells of angioimmunoblastic T-cell lymphoma and is a postulated diagnostic marker in chronic lymphocytic leukemia...

  • Lymphocyte Rosettes in an Aspirate of Bone Marrow Involved by T-Cell-Rich B-Cell Lymphoma (TCRBCL). Ansari, N. A.; Al Ajmi, A.; Shome, D. K. // International Journal of Surgical Pathology;Oct2005, Vol. 13 Issue 4, p361 

    Presents diagnostic images showing lymphocyte rosettes in an aspirate of bone marrow involved by T-cell-rich B-cell lymphoma in a 39-year-old Bahrani man presented with a 10-month history of progressive weight loss, jaundice, and abdominal fullness. Results of physical examination; Computed...

  • EZH2 Codon 641 Mutations are Common in BCL2-Rearranged Germinal Center B Cell Lymphomas. Ryan, Russell J. H.; Nitta, Mai; Borger, Darrell; Zukerberg, Lawrence R.; Ferry, Judith A.; Harris, Nancy Lee; Iafrate, A. John; Bernstein, Bradley E.; Sohani, Aliyah R.; Long Phi Le // PLoS ONE;2011, Vol. 6 Issue 12, p1 

    Mutations at codon 641 of EZH2 are recurrent in germinal center B cell lymphomas, and the most common variants lead to altered EZH2 enzymatic activity and enhanced tri-methylation of histone H3 at lysine 27, a repressive chromatin modification. As an initial step toward screening patients for...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics