Tartrate-Resistant Acid Phosphatase as an Immunohistochemical Marker for Inflammatory Macrophages

Stephen Slone; Sheron Lear; Alvin Martin
April 2007
American Journal of Clinical Pathology;Apr2007, Vol. 127 Issue 4, p556
Academic Journal
Human serum contains 2 isoforms of type-5 tartrate-resistant acid phosphatase (TRACP): 5a and 5b. TRACP-5b is osteoclastic. Our goal was to determine if serum TRACP-5a could originate from inflammatory macrophages (MΦ). We stained 246 paraffin-embedded tissue samples for TRACP using monoclonal antibody 9C5 (mab9C5) to isoforms 5a and 5b and a novel mab220 specific to isoform 5a. CD68 and lysozyme were also stained. MΦ of chronic and granulomatous inflammation and in tissues that undergo strong antigenic stimulation were strongly positive for TRACP, more so with mab220 than with mab9C5. Noninflammatory MΦ in lymph node sinuses or germinal centers and red pulp MΦ of spleen were weak or negative for TRACP. Marginal zone lymphocytes and sebaceous glands of skin were weakly positive for TRACP. Tissue mast cells displayed strong TRACP staining. Neuroendocrine cells of gastrointestinal tissues were strongly immunoreactive with mab9C5 but negative with mab220. Restricted expression of TRACP primarily in inflammatory MΦ supports our hypothesis that circulating TRACP-5a could be a biomarker of chronic inflammatory disease activity.


Related Articles

  • LN-5 antibody against human macrophages cross-reacts with routinely processed human sebaceous glands. Lis, Grzegorz J.; Litwin, Jan A.; Furgał-Borzych, Alicja; Pyka-Fościak, Grażyna // Folia Histochemica et Cytobiologica;2012, Vol. 50 Issue 2, p319 

    LN-5 monoclonal antibody against human macrophages was found to selectively stain human sebaceous glands in formalin-fixed, paraffin-embedded skin samples. Undifferentiated sebocyte progenitors were negative, and only sebocytes from the onset of their differentiation revealed positive...

  • Human intestinal versus tissue-nonspecific alkaline phosphatase as complementary urinary markers for the proximal tubule. Nouwen, Etienne J.; De Broe, Marc E. // Kidney International Supplement;Nov1994, Issue 47, pS-43 

    The availability of early biological markers of renal damage is important an for he identification of risk factors and far starling therapeutic intervention in the reversible phase of renal pathology. Tile usefulness of such markers relies upon their capacity to detect alterations in distinct...

  • Appearance of T-Cell Subpopulations During the Time Course of Beryllium-Induced Granulomas. Maceira, Juan M. P.; Fukuyama, Kimie; Epstein, William L. // Journal of Investigative Dermatology;Nov84, Vol. 83 Issue 5, p314 

    Employing monoclonal antibodies that identify T- helper/inducer or T-suppressor/cytotoxic cells, we investigated the T-lymphocyte subsets in hypersensitivity granulomas developed after beryllium salt injection. T-helper/inducer cells were detected at all stages of the lesions, including early...

  • Intradermal Injection of Propionibacterium acnes: A Model of Inflammation Relevant to Acne. De Young, Larry M.; Young, John M.; Ballaron, Stephen J.; Spires, Doreen A.; Puhvel, S. Madli // Journal of Investigative Dermatology;Nov84, Vol. 83 Issue 5, p394 

    The intradermal injection of 140 μg of Propionibacterium acnes (CN 6134) into the ears of female Sprague-Dawley rats produced a chronic inflammation with formation of acneiform lesions. Inflammation was characterized by more than a doubling of ear thickness at 24 h and a peak of 3-4...

  • T-regulatory cells in severe atopic dermatitis: alterations related to cytokines and other lymphocyte subpopulations. Samochocki, Zbigniew; Alifier, Marek; Bodera, Paweł; Jeziorkowska, Renata; Rosiak, Ewa; Jurkiewicz, Beata; Glińska, Olga; Gliński, Wiesław; Stankiewicz, Wanda // Archives of Dermatological Research;Dec2012, Vol. 304 Issue 10, p795 

    The changes in lymphocyte subpopulations in atopic dermatitis (AD) concern also T-regulatory cells. We investigated the expression of various surface receptors on CD3CD4CD25FoxP3 T-regulatory cells and the activation CD28 receptor and the inhibitory CD152 receptor on helper/inducer as well as...

  • Development of a Sensitive Enzyme-Linked Immunosorbent Assay for Detection of Hepatitis B Surface Antigen Using Novel Monoclonal Antibodies. Yazdani, Yaghoub; Roohi, Azam; Khoshnoodi, Jalal; Shokri, Fazel // Avicenna Journal of Medical Biotechnology;Oct-Dec2010, Vol. 2 Issue 4, p207 

    Hepatitis B virus (HBV) infection is the 10th leading cause of death worldwide. The most important diagnostic and screening marker for HBV infection is Hepatitis B surface antigen (HBsAg), and the most widely used HBsAg screening test is Enzyme-linked Immunosorbent Assay (ELISA). In this study,...

  • Immunohistochemical staining for adipophilin, perilipin and TIP47. K Muthusamy // Journal of Clinical Pathology;Nov2006, Vol. 59 Issue 11, p1166 

    BACKGROUND: The presence of lipid in the cell cytoplasm is useful for supporting the diagnosis of sebaceous gland carcinoma (SGC). Currently this requires histochemical stains that are carried out on frozen sections of unprocessed tissue. Recently, several anti-adipocytic antibodies that...

  • Systemic dysregulation of CEACAM1 in melanoma patients. Markel, Gal; Ortenberg, Rona; Seidman, Rachel; Sapoznik, Sivan; Koren-Morag, Nira; Besser, Michal J.; Bar, Jair; Shapira, Ronnie; Kubi, Adva; Nardini, Gil; Tessone, Ariel; Treves, Avraham J.; Winkler, Eyal; Orenstein, Arie; Schachter, Jacob // Cancer Immunology, Immunotherapy;Feb2010, Vol. 59 Issue 2, p215 

    It was previously shown that CEACAM1 on melanoma cells strongly predicts poor outcome. Here, we show a statistically significant increase of serum CEACAM1 in 64 active melanoma patients, as compared to 48 patients with no evidence of disease and 37 healthy donors. Among active patients, higher...

  • Endogenous Antibodies for Tumor Detection. Rich, Barrie S.; Honeyman, Joshua N.; Darcy, David G.; Smith, Peter T.; Williams, Andrew R.; Lim, Irene Isabel P.; Johnson, Linda K.; Gönen, Mithat; Simon, Joel S.; LaQuaglia, Michael P.; Simon, Sanford M. // Scientific Reports;5/30/2014, p1 

    The study of cancer immunology has provided diagnostic and therapeutic instruments through serum autoantibody biomarkers and exogenous monoclonal antibodies. While some endogenous antibodies are found within or surrounding transformed tissue, the extent to which this exists has not been entirely...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics