TITLE

Determination of Ultrafilterable Prolactin: Elimination of Macroprolactin Interference With a Monomeric Prolactin-Selective Sample Pretreatment

AUTHOR(S)
Quinn, Adam M.; Rubinas, Tara C.; Garbincius, C. Jean; Holmes, Earle W.
PUB. DATE
December 2006
SOURCE
Archives of Pathology & Laboratory Medicine;Dec2006, Vol. 130 Issue 12, p1807
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Context.�Macroprolactin (macroPRL), present in as many as 25% of serum specimens with elevated serum prolactin concentrations, can cause apparent hyperprolactinemia in the absence of clinical features and lead to unnecessary clinical, laboratory, and neuroradiological workups. Objective.�To develop an ultrafiltration method that eliminates macroPRL interference from PRL immunoassays. Design.�The method involves centrifugation of undiluted serum in a Centricon-100 filter device followed by a PRL assay of the serum ultrafiltrate. Results.�Ultrafiltrates prepared by this technique are devoid of gamma globulins and contain (mean � SE) 19% � 7% of the albumin concentration of the original serum. These ultrafiltrates contain 85% � 7% of the total PRL immunoreactivity of serum spiked with 23 kd recombinant human prolactin (rHuPRL) and less than 2% of the 50 kd big PRL (bPRL) of whole serum. The fractional recovery of ultrafilterable PRL (uPRL) from serum samples of 54 female patients was 0.78 (confidence interval 0.73-0.83) of the total. The run-to-run coefficient of variation of the uPRL assay was 4.3%. The uPRL concentration (mean � SD) in a group of healthy female controls was 8.0 � 3.1 ng/mL. Conclusions.�Ultrafiltration is a rapid and simple method for eliminating analytical interference by macroPRL. Ultrafiltrates can be analyzed by most, if not all, currently available PRL immunoassays and represent a practical and precise alternative to gel filtration chromatography for the estimation of the monomeric prolactin concentration of serum.
ACCESSION #
24292523

Tags: PROLACTIN;  SERUM;  PROLACTINOMA;  ULTRAFILTRATION;  CHROMATOGRAPHIC analysis;  IMMUNOASSAY

 

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