TITLE

Determination, by inductively coupled plasma mass spectrometry, of changes in cellular metal content resulting from herpes simplex virus-1 (HSV-1) infection

AUTHOR(S)
Cafferky, Katie DeNicola; Thompson, Richard L.; Richardson, Douglas D.; Caruso, Joseph A.
PUB. DATE
March 2007
SOURCE
Analytical & Bioanalytical Chemistry;Mar2007, Vol. 387 Issue 6, p2037
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Metals and metal-containing compounds are known to play important roles in many biological processes, including metabolic and detoxification pathways and the formation and function of proteins. Like all organisms, viruses are expected to contain different metals. These metals, either by themselves or in the form of metalloproteins, may be involved in the virus’s ability to infect healthy cells and replicate within them. Identification and speciation of metals in control cells and in cells affected by a virus could be helpful in elucidating infection and replication mechanisms; these might, in turn, be vital to the development of more effective treatments. There has, however, been no extensive investigation of the metals specific viruses contain or affect. The objective of this study was to investigate changes in cellular metal content resulting from herpes simplex virus 1 (HSV-1) infection. Inductively coupled plasma mass spectrometry was used to identify differences between metal concentrations in uninfected and HSV-1-infected mammalian cells. Although it can be assumed that decreases in metal content are a result of cellular response to the virus, increases can be attributed either to cellular response or to the HSV-1 virus itself. Microwave digestion and flow injection methods suitable for small sample volumes were used, and the effects of different virus inactivation procedures were explored. This work is the first step in the identification of metals pertinent to HSV-1 infection and lays the foundation for future studies concentrating on characterization of these metal-associated or containing molecules.
ACCESSION #
24207445

 

Related Articles

  • A Metalloproteomics Study on the Association of Mercury With Breast Milk in Samples From Lactating Women in the Amazon Region of Brazil. Santos, Felipe; Cavecci, Bruna; Vieira, José; Franzini, Vanessa; Santos, Ademir; Lima Leite, Aline; Buzalaf, Marília; Zara, Luiz; Magalhães Padilha, Pedro // Archives of Environmental Contamination & Toxicology;Aug2015, Vol. 69 Issue 2, p223 

    This study aimed to identify metalloproteins that lose their metal ions in the presence of mercury (Hg) and bind to Hg in breast milk samples collected from the riverine population of the Madeira River, a tributary of the Amazon River. Initially, total Hg was determined from the hair of...

  • Effects of Trace Metal Profiles Characteristic for Autism on Synapses in Cultured Neurons. Hagmeyer, Simone; Mangus, Katharina; Boeckers, Tobias M.; Grabrucker, Andreas M. // Neural Plasticity;2/23/2015, Vol. 2015, p1 

    Various recent studies revealed that biometal dyshomeostasis plays a crucial role in the pathogenesis of neurological disorders such as autism spectrum disorders (ASD). Substantial evidence indicates that disrupted neuronal homeostasis of different metal ions such as Fe, Cu, Pb, Hg, Se, and Zn...

  • Concentration of Metals and Other Elements in the Hair of Easter Islanders. Nakadaira, Hiroto; Serra, Ivan; Yamamoto, Masaharu; Rogers, Ruth; Gutierrez, David // Archives of Environmental Health;Jan/Feb2002, Vol. 57 Issue 1, p85 

    Evaluates a possible excess or deficiency of metals and other elements of Easter Islanders. Risk of toxicity by metals and other elements among indigenous people; Analysis of the concentrations of 39 elements in hair by inductive coupled plasma mass spectrometry; Indication that the elements...

  • Iron, Copper, and Zinc Transport: Inhibition of Divalent Metal Transporter 1 (DMT1) and Human Copper Transporter 1 (hCTR1) by shRNA. Espinoza, Alejandra; Blanc, Solange; Olivares, Manuel; Pizarro, Fernando; Ruz, Manuel; Arredondo, Miguel // Biological Trace Element Research;May2012, Vol. 146 Issue 2, p281 

    Iron (Fe), copper (Cu), and zinc (Zn) fulfill various essential biological functions and are vital for all living organisms. They play important roles in oxygen transport, cell growth and differentiation, neurotransmitter synthesis, myelination, and synaptic transmission. Because of their role...

  • High Metal Ion Levels After Use of the ASRâ„¢ Device Correlate With Development of Pseudotumors and T Cell Activation. Hailer, Nils; Bengtsson, Mats; Lundberg, Christina; Milbrink, Jan // Clinical Orthopaedics & Related Research;Mar2014, Vol. 472 Issue 3, p953 

    Background: Pseudotumors and immunologic alterations are reported in patients with elevated metal ion levels after resurfacing arthroplasty of the hip. A direct association of increased cobalt and chromium concentrations with the development of pseudotumors has not been established....

  • Placental Transfer of Heavy Metals in Normal Pregnant Japanese Women. Tsuchiya, Hironobu; Mitani, Kazunori; Kodama, Kyoko; Nakata, Toshikazu // Archives of Environmental Health;Jan/Feb84, Vol. 39 Issue 1 

    Examines the placental transfer of heavy metals in pregnant women in Nagoya City, Japan. Ratio of metals in the maternal and cord blood; Ability of cadmium to induce biosynthesis of metallothionein and inorganic mercury; Types of metals evident in the maternal blood.

  • Cadmium Levels in the Lung, Liver, Kidney Cortex, and Urine Samples from Australians without Occupational Exposure to Metals. Satarug, Soisungwan; Baker, Jason R.; Reilly, Paul E. B.; Moore, Michael R.; Williams, David J. // Archives of Environmental Health;Jan/Feb2002, Vol. 57 Issue 1, p69 

    ABSTRACT. The authors undertook this study to assess levels of cadmium exposure in the general population. Samples of lung, liver, and kidney were obtained from 61 cadavers (43 males, 18 females; 2–89 yr of age, mean age = 38.5 yr) who died from accidental causes and who were subject to...

  • A Child with Chronic Manganese Exposure from Drinking Water.  // Environmental Health Perspectives;Jun2002, Vol. 110 Issue 6, p613 

    Examines the toxic effects of manganese. Elevation of manganese concentrations in whole blood, urine and hair; Effect of high concentration of manganese on learning; Use of psychometric testing to evaluate the IQ and verbal and visual memory.

  • Is living proof enough? Yu, Winifred // Natural Health;Apr99, Vol. 29 Issue 3, p64 

    Provides information on chelation, a process first developed to remove metals from the body. Factors that may explain the success of chelation; Increase in the body's free radical population by too many metallic ions in the body; Criticisms and praises for the process.

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics