Multivariate curve resolution of rapid-scan FTIR difference spectra of quinone photoreduction in bacterial photosynthetic membranes

Blanchet, L.; Mezzetti, A.; Ruckebusch, C.; Huvenne, J.-P.; De Juan, A.
March 2007
Analytical & Bioanalytical Chemistry;Mar2007, Vol. 387 Issue 5, p1863
Academic Journal
Photosynthetic reaction centres and membranes are systems of particular interest and are often taken as models to investigate the molecular mechanisms of selected bioenergetic reactions. In this work, a multivariate curve resolution by alternating least squares procedure is detailed for resolution of time-resolved difference FTIR spectra probing the evolution of quinone reduction in photosynthetic membranes from Rhodobacter sphaeroides under photoexcitation. For this purpose, different data sets were acquired in the same time range and spectroscopic domain under slightly different experimental conditions. To enable resolution and provide meaningful results the different data sets were arranged in an augmented matrix. This strategy enabled recovery of three different species despite rank-deficiency conditions. It also results in better definition (identity and evolution) of the contributions. From the resolved spectra, the species have been attributed to: 1. the formation of ubiquinol, more precisely the disappearance of Q/appearance of QH2; 2. conformational change of the protein in the surrounding biological medium; 3. oxidation of diaminodurene, a redox mediator. Because, moreover, results obtained from augmented data sets strategies enable quantitative and qualitative interpretation of concentration profiles, other effects, for example the consequence of repeated light excitation of the same sample, choice of illumination power, or the number of spectra accumulated could be compared and discussed.


Related Articles

  • Conductive Cotton Prepared by Polyaniline In Situ Polymerization Using Laccase. Zhang, Ya; Dong, Aixue; Wang, Qiang; Fan, Xuerong; Cavaco-Paulo, Artur; Zhang, Ying // Applied Biochemistry & Biotechnology;Sep2014, Vol. 174 Issue 2, p820 

    The high-redox-potential catalyst laccase, isolated from Aspergillus, was first used as a biocatalyst in the oxidative polymerization of water-soluble conductive polyaniline, and then conductive cotton was prepared by in situ polymerization under the same conditions. The polymerization of...

  • Infrared spectroscopy of acetone–water liquid mixtures. I. Factor analysis. Max, Jean-joseph; Chapados, Camille // Journal of Chemical Physics;9/15/2003, Vol. 119 Issue 11, p5632 

    Acetone and water mixtures covering the whole solubility range were measured by Fourier transform infrared attenuated total reflectance spectroscopy. In this system, only water can supply the hydrogen atoms necessary for hydrogen bonding. Using spectral windowing with factor analysis (FA), 10...

  • Electrochemical Synthesis and Characterization of a New Electrochromic Copolymer Based on 2,2'-Bithiophene and Tris[4-(2-Thienyl)phenyl]amine. Shuang Chen; Qingxin Gao; Jinsheng Zhao; Chuansheng Cui; Wei Yang; Xianxi Zhang // International Journal of Electrochemical Science;Oct2012, Vol. 7 Issue 10, p9095 

    A novel copolymer based on tris[4-(2-thienyl)phenyl]amine (TTPA) and 2,2'-bithiophene (BT) was electrochemically synthesized.Characterizations of the resulting copolymer P(TTPA-co-BT) were performed by cyclic voltammetry (CV), UV-vis spectroscopy, Fourier transform infrared spectroscopy(FT-IR),...

  • Chemical modifications and characteristic changes in bacterial cellulose treated with different media. Wu, Jian; Zheng, Yudong; Yang, Zhou; Cui, Qiuyan; Wang, Qiaoli; Gao, Shuang; Ding, Xun // Journal of Polymer Research;Sep2012, Vol. 19 Issue 9, p1 

    Bacterial cellulose membranes have attracted a great deal of attention as novel biomedical materials. In this paper, bacterial cellulose was treated with acidic, alkaline, and redox solutions to investigate the subsequent changes in the characteristics of the cellulose. The chemical structure,...

  • Interface for time-resolved electrochemical infrared microspectroscopy using synchrotron infrared radiation. Rosendahl, Scott M.; Borondics, Ferenc; May, Tim E.; Pedersen, Tor M.; Burgess, Ian J. // Review of Scientific Instruments;Aug2011, Vol. 82 Issue 8, p083105 

    A description of a coupled electrochemical and spectrometer interface using synchrotron infrared radiation is provided. The interface described allows for the precise and accurate timing needed for time-resolved IR spectroscopic studies of electrochemical systems. The overall interface uses a...

  • Electrochemical Detection of Hydrogen Peroxide Based on Graphene Oxide/Prussian Blue Modified Glassy Carbon Electrode. Farah, Abdullahi Mohamed; Thema, Force Tefo; Dikio, Ezekiel Dixon // International Journal of Electrochemical Science;Jun2012, Vol. 7 Issue 6, p5069 

    Prussian blue (PB) and graphene oxide (GO) were synthesized and characterized by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy among others. The synthesized Prussian blue and graphene oxide were used in the modification of glass carbon electrodes for the chemical...

  • Preparation of TiO2-Fullerene Composites and Their Photocatalytic Activity under Visible Light. Katsumata, Ken-ichi; Matsushita, Nobuhiro; Okada, Kiyoshi // International Journal of Photoenergy;2012, Special section p1 

    The development of visible light-sensitive photocatalytic materials is being investigated. In this study, the anatase and rutile-C60 composites were prepared by solution process. The characterization of the samples was conducted by using XRD, UV-vis, FT-IR, Raman, and TEM. The photocatalytic...

  • On the Development of Thermally Stable Semi-IPNs of PVA and PAN using IR, DSC and XRD Studies. Deshpande, Deepti S.; Bajpai, R.; Bajpai, A. K. // AIP Conference Proceedings;7/16/2011, Vol. 1349 Issue 1, p180 

    A novel biopolymer based Semi-IPN of polyvinyl alcohol (PVA) and polycrylonitrile (PAN) were synthesized in various proportions by redox polymerization and analyzed thermally by differential scanning calorimetry (DSC). DSC thermograms shows good thermal stability, as a result of incorporation of...

  • The effect of natural organic matter on the formation and solubility of M(OH) solid phases. Antoniou, Stella; Pashalidis, Ioannis // Journal of Radioanalytical & Nuclear Chemistry;Jan2014, Vol. 299 Issue 1, p791 

    In this study the formation of M(OH) solid phases (e.g. Th(IV), Ce(IV) and Zr(IV)) has been investigated as a function of the natural organic matter (NOM) concentration in 0.1 M NaClO, in the pH range between 2 and 5, and under normal atmospheric conditions. M(OH) has been prepared by alkaline...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics