Continuous nondestructive monitoring of Bordetella pertussis biofilms by Fourier transform infrared spectroscopy and other corroborative techniques

Serra, Diego; Bosch, Alejandra; Russo, Daniela M.; Rodríguez, María E.; Zorreguieta, Ángeles; Schmitt, Juergen; Naumann, Dieter; Yantorno, Osvaldo
March 2007
Analytical & Bioanalytical Chemistry;Mar2007, Vol. 387 Issue 5, p1759
Academic Journal
This work describes the application of several analytical techniques to characterize the development of Bordetella pertussis biofilms and to examine, in particular, the contribution of virulence factors in this development. Growth of surface-attached virulent and avirulent B. pertussis strains was monitored in continuous-flow chambers by techniques such as the crystal violet method, and nondestructive methodologies like fluorescence microscopy and Fourier transform (FT) IR spectroscopy. Additionally, B. pertussis virulent and avirulent strains expressing green fluorescent protein were grown adhered to the base of a glass chamber of 1-μm thickness. Three-dimensional images of mature biofilms, acquired by confocal laser scanning microscopy, were quantitatively analysed by means of the computer program COMSTAT. Our results indicate that only the virulent (Bvg+) phase of B. pertussis is able to attach to surfaces and develop a mature biofilm. In the virulent phase these bacteria are capable of producing a biofilm consisting of microcolonies of approximately 200 μm in diameter and 24 μm in depth. FTIR spectroscopy allowed us not only to follow the dynamics of biofilm growth through specific biomass and biofilm marker absorption bands, but also to monitor the maturation of the biofilm by means of the increase of the carbohydrate-to-protein ratio.


Related Articles

  • Characterization of Bordetella pertussis growing as biofilm by chemical analysis and FT-IR spectroscopy. Bosch, A.; Serra, D.; Prieto, C.; Schmitt, J.; Naumann, D.; Yantorno, O. // Applied Microbiology & Biotechnology;Aug2006, Vol. 71 Issue 5, p736 

    Although Bordetella pertussis, the etiologic agent of whooping cough, adheres and grows on the ciliated epithelium of the respiratory tract, it has been extensively studied only in liquid cultures. In this work, the phenotypic expression of B. pertussis in biofilm growth is described as a first...

  • Stability of aqueous silica nanoparticle dispersions. Metin, Cigdem; Lake, Larry; Miranda, Caetano; Nguyen, Quoc // Journal of Nanoparticle Research;Feb2011, Vol. 13 Issue 2, p839 

    In this study, we present quantification methods for nanoparticle stability analysis using non-intrusive analytical techniques: attenuated total reflectance, Fourier transform infrared (ATR-FTIR) spectroscopy, ultraviolet-visible (UV-vis) spectrophotometer, zeta potential analyses, and dynamic...

  • 'Biofilmology': a multidisciplinary review of the study of microbial biofilms. Karunakaran, Esther; Mukherjee, Joy; Ramalingam, Bharathi; Biggs, Catherine A. // Applied Microbiology & Biotechnology;Jun2011, Vol. 90 Issue 6, p1869 

    The observation of biofilm formation is not a new phenomenon. The prevalence and significance of biofilm and aggregate formation in various processes have encouraged extensive research in this field for more than 40 years. In this review, we highlight techniques from different disciplines that...

  • Enhanced pullulan production in a biofilm reactor by using response surface methodology. Kuan-Chen Cheng; Demirci, Ali; Catchmark, Jeffrey M. // Journal of Industrial Microbiology & Biotechnology;Jun2010, Vol. 37 Issue 6, p587 

    Pullulan is a linear homopolysaccharide that is composed of glucose units and often described as α-1, 6-linked maltotriose. In this study, response surface methodology using Box–Behnken design was employed to study the effects of sucrose and nitrogen concentrations on pullulan...

  • Comparison of polycaprolactone and starch/polycaprolactone blends as carbon source for biological denitrification. Shen, Z.; Hu, J.; Wang, J.; Zhou, Y. // International Journal of Environmental Science & Technology (IJE;Apr2015, Vol. 12 Issue 4, p1235 

    The cross-linked starch/polycaprolactone (SPCL10) and starch/polycaprolactone (SPCL12) blends were prepared, characterized and used as carbon source and biofilm support for biological nitrate removal. The results showed that SPCL10 and SPCL12 had similar performance on water absorption (about 21...

  • Investigating the heterogeneity of cell growth in microbial colonies by FTIR microspectroscopy. Thi, N. A. Ngo; Naumann, D. // Analytical & Bioanalytical Chemistry;Mar2007, Vol. 387 Issue 5, p1769 

    Microorganisms rarely occur as individual cells in nature and are, instead, organized in complex multicellular communities such as colonies, fruiting bodies, or biofilms. Interest in the natural microbial life-style has increased during the last decade and a whole plethora of techniques has been...

  • Usnic acid, a lichen secondary metabolite inhibits Group A Streptococcus biofilms. Nithyanand, Paramasivam; Beema Shafreen, Raja; Muthamil, Subramanian; Karutha Pandian, Shunmugiah // Antonie van Leeuwenhoek;Jan2015, Vol. 107 Issue 1, p263 

    Group A Streptococci (GAS) are involved in a number of life threatening diseases and biofilm formation by these pathogens are considered as an important virulence determinant as it mediates antibiotic resistance among them. In the present study, we have explored the ability of (+)-usnic acid, a...

  • A watermark strategy for quantum images based on quantum fourier transform. Zhang, Wei-Wei; Gao, Fei; Liu, Bin; Wen, Qiao-Yan; Chen, Hui // Quantum Information Processing;Feb2013, Vol. 12 Issue 2, p793 

    We present a robust watermark strategy for quantum images. The watermark image is embedded into the fourier coefficients of the quantum carrier image, which will not affect the carrier image's visual effect. Before being embedded into the carrier image, the watermark image is preprocessed to be...

  • Surface analysis of paper documents damaged by foxing. Buzio, R.; Calvini, P.; Ferroni, A.; Valbusa, U. // Applied Physics A: Materials Science & Processing;2004, Vol. 79 Issue 2, p383 

    The causes of foxing, a rust-red spotting of engravings, books and archive documents, are not yet completely understood, but they are usually ascribed to mould growth and/or heavy-metal-induced degradation of cellulose and sizing materials. In the present work we report the use of attenuated...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics