TITLE

A binary matrix for background suppression in MALDI-MS of small molecules

AUTHOR(S)
Zhong Guo; Lin He
PUB. DATE
March 2007
SOURCE
Analytical & Bioanalytical Chemistry;Mar2007, Vol. 387 Issue 5, p1939
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Application of matrix-assisted laser-desorption ionization mass spectrometry (MALDI-MS) to small-molecule detection is often limited, because of high matrix background signals in the low-mass region. We report here an approach in which a mixture of two conventional MALDI matrices with different proton affinity was used to suppress the formation of matrix clusters and fragments. Specifically, when acidic α-cyano-4-hydroxycinnamic acid (CHCA) and basic 9-aminoacridine (9-AA) were used as the binary matrix, fewer background matrix peaks were observed in both positive and negative-mode detection of small molecules. In addition, the presence of CHCA substantially reduced the laser fluence needed for analyte desorption and ionization; thus better signal-to-background ratios were observed for negatively charged inositol phosphates in complex plant extracts. [Figure not available: see fulltext.]
ACCESSION #
24109140

 

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