TITLE

Aspirin-Induced Apoptosis in Human Gastric Cancer Epithelial Cells: Relationship with Protein Kinase C Signaling

AUTHOR(S)
Maria Redlak; Jacinda Power
PUB. DATE
March 2007
SOURCE
Digestive Diseases & Sciences;Mar2007, Vol. 52 Issue 3, p810
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Abstract??This study examined the relationship of protein kinase C (PKC) signaling with apoptosis induced by aspirin (ASA) in gastric surface cancer cells (AGS cell line). We found increased expression of two PKC isoforms (? and ?II) that translocated from the cytosol into the cell membrane fraction after ASA (40?mM) stimulation. PKC ?Iexpression markedly decreased in response to ASA treatment. This process was independent of caspase activation because no caspase inhibitors used (i.e., inhibitors to caspase 3, 6, 7, 8, and total caspase activity) significantly changed PKC processing, although inhibition of caspase cascade activity markedly attenuated the apoptosis induced by ASA as measured by DNA-histone complex formation. Upstream PKC signaling induced by ASA seems to play an important role in the regulation of apoptosis because PKC inhibitors significantly reduced the magnitude of DNA-histone complex formation. We conclude that ASA-induced apoptosis in gastric cancer cells is mediated, at least in part, through a PKC mechanism involving the (?) and (?) isoforms and that PKC signaling operates upstream of the caspase cascade, which when activated elicits its downstream effects on DNA degradation.
ACCESSION #
24079666

 

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