Probiotic Effects on 5-Fluorouracil-Induced Mucositis Assessed by the Sucrose Breath Test in Rats

Chad Mauger; Ross Butler; Mark Geier; Katie Tooley; Gordon Howarth
March 2007
Digestive Diseases & Sciences;Mar2007, Vol. 52 Issue 3, p612
Academic Journal
Abstract??The sucrose breath test (SBT) was employed to noninvasively assess the efficacy of probiotics in 5-fluorouracil (5-FU)-induced intestinal mucositis. Dark Agouti rats were allocated to 5 groups (n=10): 5-FU+L. fermentumBR11, 5-FU+L. rhamnosusGG, 5-FU+B. lactisBB12, 5-FU+skim milk (SM), and saline+SM. Probiotics were administered by oral gavage for 10 days. Mucositis was induced on day 7 by intraperitoneal injection of 5-FU (150?mg/kg) or vehicle (saline). Rats were sacrificed 72?h after 5-FU injection. The SBT measured breath13CO2(expressed as percentage cumulative dose at 90?min; %CD90) on days 0, 7, and 10. %CD90 was significantly lower in 5-FU-treated controls compared with that in saline-treated controls on day 10. 5-FU caused an 83% reduction in sucrase and a 510% increase in MPO activity. The SBT detected damage induced by 5-FU and is a simple, noninvasive indicator of small bowel injury. The probiotics assessed offered no protection from mucositis at the dose tested.


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