Determination of pharmaceuticals from different therapeutic classes in wastewaters by liquid chromatography—electrospray ionization—tandem mass spectrometry

Botitsi, Eleni; Frosyni, Charalampia; Tsipi, Despina
February 2007
Analytical & Bioanalytical Chemistry;Feb2007, Vol. 387 Issue 4, p1317
Academic Journal
A sensitive analytical method has been developed and validated for simultaneous determination of pharmaceuticals from different therapeutic classes, i.e. five sulfonamide (SA) and trimethoprim antimicrobials and the anti-inflammatory drug diclofenac, in effluent wastewaters at trace levels. Effluent samples from treatment of wastewater were enriched by solid-phase extraction (SPE) using the Waters Oasis HLB cartridge. The analytes were identified and quantified by reversed-phase liquid chromatography–tandem mass spectrometry operated in the selected reaction monitoring (SRM) mode, using positive electrospray ionization. The pharmaceuticals were, consequently, quantified both by use of isotopically labelled internal standards and by standard addition methods to address the issue of matrix effects related to signal suppression by co-eluting compounds. Average recoveries from fortified samples were usually >70%, with relative standard deviations below 20%. Method detection limits in wastewater matrices were between 7.0 and 10 ng L−1. Identification points (IPs) were used for unequivocal identification of target analytes in real samples. Diclofenac, trimethoprim, and sulfamethoxazole were mainly detected, in the concentration range 10 to 400 ng L−1, in effluent samples collected from four different sewage-treatment plants in Greece.


Related Articles

  • Occurrence patterns of pharmaceuticals in water and wastewater environments. Nikolaou, Anastasia; Meric, Sureyya; Fatta, Despo // Analytical & Bioanalytical Chemistry;Feb2007, Vol. 387 Issue 4, p1225 

    The occurrence of pharmaceuticals and their metabolites and transformation products in the environment is becoming a matter of concern, because these compounds, which may have adverse effects on living organisms, are extensively and increasingly used in human and veterinary medicine and are...

  • Liquid chromatography—tandem mass spectrometry of bioactive pharmaceutical compounds in the aquatic environment–a decade’s activities. Chunyan Hao; Clement, Ray; Yang, Paul // Analytical & Bioanalytical Chemistry;Feb2007, Vol. 387 Issue 4, p1247 

    The occurrence of bioactive pharmaceutical compounds in the aquatic environment is an emerging concern. Liquid chromatography, coupled with mass spectrometry (LC–MS) or tandem mass spectrometry (LC–MS–MS) using an electrospray ionization interface, is a crucial tool for...

  • Stay Away from the Cliffs! Dolan, John W. // LC-GC Europe;Feb2005, Vol. 18 Issue 2, p64 

    The article examines a couple of liquid chromatography methods that could be operating too close to the edge. The first method that the author of this article encountered is several years ago when he was visiting a pharmaceutical production facility. One of the chemists brought him a problem...

  • SCREENING, ISOLATION AND PURIFICATION OF ANTIBIOTIC(S) FROM SCREENING, ISOLATION AND PURIFICATION OF ANTIBIOTIC(S) FROM MARINE ACTINOMYCETES. Attimarad, S. L.; Gaviraj, E. N.; Nagesh, C.; Kugaji, M. S.; Sutar, R. S. // International Journal of Research in Ayurveda & Pharmacy;May2012, Vol. 3 Issue 3, p447 

    As marine environmental conditions are extremely different from terrestrial ones, it is surmised that marine actinomycetes might produce novel bioactive compounds. Hence marine sediments, collected from the coastal areas of Gokharna and Muradeshwara of Karnataka state, were screened. Seventeen...

  • Estimation of Rizatriptan benzoate by RP-HPLC method in bulk and dosage form. Devprakash; V., Sumalatha B.; Yadav, Suhas Gurav. Prithviraj S.; P., Senthilkumar G. // Journal of Pharmacy Research;2012, Vol. 5 Issue 1, p120 

    A simple reverse phase HPLC method was developed for the estimation of Rizatriptan benzoate in bulk and dosage form. Chromatography was performed by gradient reverse phase separation on a Stainless steel column 150 x 4.6 mm, symmetry column packed with octa decyl silane bonded to porous silica...

  • Determination of rofecoxib, in the presence of its photodegradation product, in pharmaceutical preparations by micellar electrokinetic capillary chromatography. Nemutlu, Emirhan; Özalt, Nuran; Altöz, Sacide // Analytical & Bioanalytical Chemistry;Jan2004, Vol. 378 Issue 2, p504 

    A simple and rapid micellar electrokinetic capillary chromatographic (MEKC) method for analysis of rofecoxib (ROF) and its photodegradation product (PDP) in pharmaceutical preparations has been developed and validated. Analyses were conducted in a fused silica capillary (72 cm effective length,...

  • Bioactive Amines: Aspects of Quality and Safety in Food. Ribas Rodriguez, Mariana Bacellar; da Silva Carneiro, Carla; da Silva Feijó, Marcia Barreto; Conte Júnior, Carlos Adam; Borges Mano, Sérgio // Food & Nutrition Sciences;Jan2014, Vol. 5 Issue 2, p138 

    Presented in several types of food, bioactive amines are described as organic bases of low molecular weight. They have vasoactive, psychoactive and toxicological characteristics and constitute a potential health risk. The concentration of amines formed in foods depends on the type of...

  • Counter-Current Chromatography for High Throughput Analysis of Natural Products. Shihua Wu; Junling Liang // Combinatorial Chemistry & High Throughput Screening;Dec2010, Vol. 13 Issue 10, p932 

    No abstract available.

  • Separation and identification of components of the multi-component herbal drug prostanorm. Beloborodov, V.; Zakharova, N.; Savvateev, A.; Kolkhir, V.; Voskoboinikova, I. // Pharmaceutical Chemistry Journal;Nov2011, Vol. 45 Issue 9, p556 

    Prostanorm is a multi-component Russian herbal drug comprising a mixture of the extracts of St. John's wort ( Hypericum perforatum L.), goldenrod ( Solidago canadensis L.), licorice root ( Glycyrrhiza glabra L.), and purple coneflower ( Echinacea purpurea L. Moench) rhizomes and roots in equal...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics