TITLE

Studies on interactions of programmed cell death 5 (PDCD5) and its related peptides with heparin by capillary zone electrophoresis

AUTHOR(S)
Xiaomei Ling; Yi Liu; Hui Fan; Yingcheng Zhong; Dan Li; Ying Wang
PUB. DATE
February 2007
SOURCE
Analytical & Bioanalytical Chemistry;Feb2007, Vol. 387 Issue 3, p909
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Capillary zone electrophoresis (CZE) was used to investigate interactions between heparin and programmed cell death 5 ( PDCD5), and between heparin and PDCD5-related peptides. Samples containing PDCD5, PDCD5-related peptides, and heparin at various ratios were incubated at room temperature and then separated by CZE with tris-acetate buffer at pH 7.2. Both qualitative and quantitative characterizations of the binding of PDCD5 and PDCD5-related peptides to heparin were determined. The changes in the signals of PDCD5 and PDCD5-related peptides were monitored by comparing the electropherograms of the mixtures containing PDCD5 and heparin and PDCD5-related peptides and heparin with that of PDCD5 or PDCD5-related peptides only. The binding constant of the interaction between PDCD5 and heparin was calculated as 4.17 × 104 M−1 by Scatchard analysis. Our investigations show that it is possible to characterize the interaction between PDCD5 and heparin quantitatively and the interaction between PDCD5-related peptides and heparin qualitatively using CZE.
ACCESSION #
23819311

 

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