TITLE

Chemiluminescent imaging analysis of interferon alpha in serum samples

AUTHOR(S)
Lingyan Hou; Zhujun Zhang; Lirong Luo
PUB. DATE
February 2007
SOURCE
Analytical & Bioanalytical Chemistry;Feb2007, Vol. 387 Issue 3, p925
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Enzyme-linked immunosorbent assay (ELISA), horseradish peroxidase (HRP)-catalyzed fluorescent reaction, and oxalate chemiluminescence imaging analysis have been combined to develop a sensitive, simple, and rapid method for analysis of interferon alpha (α-IFN) in human serum samples. A typical “sandwich type” immunoassay was used. Reaction of o-phenylenediamine (OPD) with hydrogen peroxide (H2O2), catalyzed by HRP, produced 2,3-diaminophenazine (PDA), which was detected by chemiluminescence imaging analysis with the bis(2,4,6-trichlorophenyl)oxalate (TCPO)–H2O2–glyoxaline–PDA chemiluminescent system. The TCPO chemiluminescent imaging system is more sensitive and the chemiluminescence quantum yield is at least five times higher than for the luminol–H2O2–HRP–PIP ( p-iodophenol) chemiluminescent imaging system. The results showed there was a very good linear correlation between response and amount of α-IFN in the range 1.3–156.0 pg mL−1 ( R = 0.9991) and the detection limit was 0.8 pg mL−1 ( S/ N=3). The relative standard deviation ( n = 9) was 4.7%. The proposed method has been used for successful analysis of the amount of α-IFN in human serum. The results obtained compared well with those obtained by conventional colorimetric ELISA and luminol chemiluminescent ELISA. [Figure not available: see fulltext.]
ACCESSION #
23819287

 

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