A pilot study on the infiltrating cells and cytokine levels in the tear of fungal keratitis patients

Vasanthi, Mani; Prajna, Namperumalsamy Venkatesh; Lalitha, Prajna; Mahadevan, Kannan; Muthukkaruppan, Veerappan
January 2007
Indian Journal of Ophthalmology;Jan2007, Vol. 55 Issue 1, p27
Academic Journal
journal article
Aim: To determine the cellular profile and cytokine levels in the tear fluid of fungal keratitis patients.Materials and Methods: Tear samples were collected from six fungal keratitis patients (Group I) from active stages of the disease up to resolution. Tears collected from the following served as controls: uninfected fellow eye (Group II A) of Group I, patients undergoing cataract surgery (Group II B) and acute conjunctivitis (Group II C). The cellular profile was evaluated. Interleukines (IL-6, IL-8 and IL-1beta) were estimated using sandwich enzyme immunoassay. Statistical analysis was carried out using nonparametric two-sample median test.Results: Polymorphonuclear leukocytes (PMN) were the predominant infiltrating cells in Group I. During the initial stages of fungal infection, levels of IL-6 and IL-8 in the tear samples were found to be significantly increased when compared with Group II A (P=0.019 for IL-6, P<0.001 for IL-8). This was also true for IL -8 (P=0.008) levels in Group I and Group II B). While IL-6 levels decreased significantly towards healing, IL-8 remained slightly elevated even after healing. These cytokines were at the base level in Group II A. Lymphocytes and PMN were present in equal proportions in Group II C, which showed elevated levels of cytokines but not to the extent of Group I.Conclusion: This horizontal study indicates that understanding the nature of the inflammatory response in the tears of fungal keratitis patients is of considerable interest and warrants further investigations.


Related Articles

  • HIV-activated human plasmacytoid DCs induce Tregs through an indoleamine 2,3-dioxygenase-dependent mechanism. Manches, Olivier; Munn, David; Fallahi, Anahita; Lifson, Jeffrey; Chaperot, Laurence; Plumas, Joel; Bhardwaj, Nina // Journal of Clinical Investigation;Oct2008, Vol. 118 Issue 10, p3431 

    Plasmacytoid DCs (pDCs) have been implicated as crucial cells in antiviral immune responses. On recognizing HIV, they become activated, secreting large amounts of IFN-alpha and inflammatory cytokines, thereby potentiating innate and adaptive antiviral immune responses. Here, we have shown that...

  • Altered plasma nerve growth factor-like immunoreactivity and nerve growth factor-receptor expression in human old age. Antonelli, A.; Bracci-Laudiero, L.; Aloe, L. // Gerontology;May/Jun2003, Vol. 49 Issue 3, p185 

    Background: Nerve growth factor (NGF), discovered because of its action on cells in the peripheral and the central nervous system, is now known to act also on immune cells in developing and adult subjects. Whether peripheral lymphocytes of aged subjects are responsive to this...

  • Neutrophil-mediated innate immune resistance to mycobacteria. Martineau, Adrian R.; Newton, Sandra M.; Wilkinson, Katalin A.; Kampmann, Beate; Hall, Bridget M.; Nawroly, Niga; Packe, Geoffrey E.; Davidson, Robert N.; Griffiths, Christopher J.; Wilkinson, Robert J. // Journal of Clinical Investigation;Jul2007, Vol. 117 Issue 7, p1988 

    Neutrophils contain antimicrobial peptides with antituberculous activity, but their contribution to immune resistance to tuberculosis (TB) infection has not been previously investigated to our knowledge. We determined differential white cell counts in peripheral blood of 189 adults who had come...

  • Understanding the slow depletion of memory CD4+ T cells in HIV infection. Yates, Andrew; Stark, Jaroslav; Klein, Nigel; Antia, Rustom; Callard, Robin // PLoS Medicine;May2007, Vol. 4 Issue 5, pe177 

    Background: The asymptomatic phase of HIV infection is characterised by a slow decline of peripheral blood CD4(+) T cells. Why this decline is slow is not understood. One potential explanation is that the low average rate of homeostatic proliferation or immune activation dictates...

  • The thought disorder questionnaire. Waring, Edward M; Neufeld, RWJ; Schaefer, B; Neufeld, R W J // Canadian Journal of Psychiatry;Feb2003, Vol. 48 Issue 1, p45 

    Objective: To describe the development of the Thought Disorder Questionnaire (TDQ), including data on reliability and validity, and to explain the questionnaire's feasibility and diagnostic accuracy.Methods: The TDQ has 6 scales, each with 10 items (on a scale from 0...

  • Reaching homeless youths for Chlamydia trachomatis and Neisseria gonorrhoeae screening in Denver, Colorado. van Leeuwen, J.M.; Rietmeijer, C.A.; LeRoux, T.; White, R.; Petersen, J. // Sexually Transmitted Infections;Oct2002, Vol. 78 Issue 5, p357 

    Background and Rationale: The availability of urine based testing for Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (GC) provides a unique opportunity to screen for these pathogens outside traditional clinical settings. The researchers investigated the feasibility of street...

  • Cytokines in innate host defense in the lung. Strieter, Robert M; Belperio, John A; Keane, Michael P // Journal of Clinical Investigation;3/1/2002, Vol. 109 Issue 5, p699 

    No abstract available.

  • Zoledronic Acid Treatment After Acute Spinal Cord Injury: Results of a Randomized, Placebo-Controlled Pilot Trial. Schnitzer, Thomas J.; Kim, Ki; Marks, Julia; Yeasted, Renita; Simonian, Narina; Chen, David // PM & R: Journal of Injury, Function & Rehabilitation;Sep2016, Vol. 8 Issue 9, p833 

    Objective: To determine the effect of intravenous zoledronic acid 5 mg on the extent and course of bone loss after spinal cord injury (SCI).Design: Double-blind, randomized, placebo-controlled parallel-group trial.Setting: Acute in-patient, tertiary-care...

  • Human challenge pilot study with Cyclospora cayetanensis. Alfano-Sobsey, Edith M.; Eberhard, Mark L.; Seed, John R.; Weber, David J.; Won, Kimberly Y.; Nace, Eva K.; Moe, Christine L. // Emerging Infectious Diseases;Apr2004, Vol. 10 Issue 4, p726 

    We describe a pilot study that attempted to infect human volunteers with Cyclospora cayetanensis. Seven healthy volunteers ingested an inoculum of Cyclospora oocysts (approximately 200-49,000 oocysts). The volunteers did not experience symptoms of gastroenteritis, and no oocysts were detected in...

  • G(2) chromosomal radiosensitivity in Danish survivors of childhood and adolescent cancer and their offspring. Curwen, G B; Winther, J F; Tawn, E J; Smart, V; Whitehouse, C A; Rees, G S; Olsen, J H; Guldberg, P; Rechnitzer, C; Schrøder, H; Bryant, P E; Sheng, X; Lee, H S; Chakraborty, R; Boice, J D // British Journal of Cancer;10/31/2005, Vol. 93 Issue 9, p1038 

    In order to investigate the relationship between chromosomal radiosensitivity and early-onset cancer, the G(2) chromosomal radiosensitivity assay was undertaken on a group of 23 Danish survivors of childhood and adolescent cancer, a control group comprising their partners and a group of 38 of...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics