Phenotypic and plasmid pattern analysis of Staphylococcus epidermidis in bacterial keratitis

Nayak, Niranjan; Satpathy, Gita; Vajpayee, Rasik B.; Mrudula, Srikantam
January 2007
Indian Journal of Ophthalmology;Jan2007, Vol. 55 Issue 1, p9
Academic Journal
journal article
Background: Staphylococcus epidermidis, a commensal of the conjunctival sac has been incriminated as the commonest etiological agent of bacterial keratitis. However, the pathogenic potential of this commensal organism is not clearly known.Aim: To determine any phenotypic, molecular markers of S. epidermidis pathogenicity in bacterial keratitis.Materials and Methods: A total of 382 corneal ulcer isolates of S. epidermidis and 87 S. epidermidis isolates from healthy eyes (controls) were studied. Speciation, biotyping and antibiotic sensitivity testing were performed by conventional methods. Tube slime and adherence tests were carried out by recommended techniques. Plasmid analysis was conducted by a standard protocol.Statistical Analysis: Chi-square test was employed for calculations.Results: Out of 382 corneal ulcer isolates (Pathogens) 284 (74.3%) belonged to biotypes I and II. Slime was detected in 164 (42.9%) of 382 pathogens vs. 21 (24.1%) of 87 controls (P<0.001). Sixty-five (39.6%) of 164 slime positive isolates were multidrug-resistant as compared to only 49 (22.4%) of 218 slime negative isolates (P<0.001). A significantly higher number i.e, 73.1% (120/164) of slime-producers possessed a 21 Kb plasmid in contrast to only 53.2% (116/218) of nonslime-producers (P<0.001). Presence of this plasmid had a statistical correlation of low significance with multidrug resistance (P=0.04). One hundred and seventy-two (45.0%) of 382 pathogens and 24 (27.6%) of the 87 controls were adherent to artificial surfaces (P=0.003) and the majority of the adherent organisms (99/172, 57.6%) were slime producers (P<0.001).Conclusions: Slime was associated with multidrug resistance in corneal ulcer isolates of S. epidermidis. The 21 Kb plasmid could determine virulence as it was responsible for slime production and adherence.


Related Articles

  • Multidrug-Resistant Staphylococcus aureus, India, 2013-2015. Kumar, Mohit // Emerging Infectious Diseases;Sep2016, Vol. 22 Issue 9, p1666 

    The article reports resistance patterns of Staphylococcus aureus collected over two years from December 2013-November 2015 from blood samples of patients admitted to one hospital in Odisha, eastern India. It says that methicillin-resistant Staphylococcus aureus is a versatile pathogen capable of...

  • Contrasting pediatric and adult methicillin-resistant Staphylococcus aureus isolates. David, Michael Z.; Crawford, Susan E.; Boyle-Vavra, Susan; Hostetler, Mark A.; Kim, Daniel C.; Daum, Robert S. // Emerging Infectious Diseases;Apr2006, Vol. 12 Issue 4, p631 

    We performed a prospective study of all inpatient and outpatient methicillin-resistant Staphylococcus aureus (MRSA) isolates identified at the University of Chicago Hospitals from November 2003 through November 2004. Differences in resistance to non-beta-lactam antimicrobial drugs were...

  • Persistence of resistant Staphylococcus epidermidis after single course of clarithromycin. Sjölund, Maria; Tano, Eva; Blaser, Martin J.; Andersson, Dan I.; Engstrand, Lars; Sjölund, Maria // Emerging Infectious Diseases;Sep2005, Vol. 11 Issue 9, p1389 

    We examined how a common therapy that includes clarithromycin affects normally colonizing Staphylococcus epidermidis. Samples from the nostrils of 5 patients receiving therapy were collected before, immediately after, 1 year after, and 4 years after treatment. From each patient and sample, S....

  • Quorum sensing in Staphylococcus infections. Yarwood, Jeremy M.; Schlievert, Patrick M. // Journal of Clinical Investigation;Dec2003, Vol. 112 Issue 11, p1620 

    Quorum sensing via the accessory gene regulator (agr) system has been assigned a central role in the pathogenesis of staphylococci, particularly Staphylococcus aureus. While the control of virulence gene expression in vitro by agr has been relatively straightforward to describe, regulation of...

  • Prevalence of inducible clindamycin resistance in clinical isolates of coagulase negative staphylococci at a tertiary care hospital. Bansal, Neha; Chaudhary, Uma; Gupta, Vivek // Annals of Tropical Medicine & Public Health;Sep/Oct2012, Vol. 5 Issue 5, p427 

    Background: Coagulase negative staphylococci (CoNS) are an important group of multi-drug resistant nosocomial pathogens. Clindamycin resistance in staphylococci can be either constitutive or inducible. Clindamycin has been used successfully to treat pneumonia, soft-tissue and musculoskeletal...

  • The Fight Against Multidrug-Resistant Bacteria. Deak, Dalia; Powers, John H.; Outterson, Kevin; Kesselheim, Aaron S. // Annals of Internal Medicine;1/3/2017, Vol. 166 Issue 1, p79 

    A response from the authors of the article "Progress in the fight against multidrug-resistant bacteria? A review of U.S. Food and Drug Administration-approved antibiotics,2010-2015" published in a 2016 issue of "Annals of Internal Medicine" is presented.

  • Community-associated methicillin-resistant Staphylococcus aureus isolates causing healthcare-associated infections. Maree, Cynthia L.; Daum, Robert S.; Boyle-Vavra, Susan; Matayoshi, Kelli; Miller, Loren G. // Emerging Infectious Diseases;Feb2007, Vol. 13 Issue 2, p236 

    We noted a marked increase in healthcare-associated (HA) methicillin-resistant Staphylococcus aureus (MRSA) infections caused by isolates phenotypically consistent with community-associated (CA)-MRSA strains. To study this trend, we retrospectively examined all HA-MRSA isolates from patients in...

  • Staphylococcus aureus Regulatory RNAs as Potential Biomarkers for Bloodstream Infections. Bordeau, Valérie; Cady, Anne; Revest, Matthieu; Rostan, Octavie; Sassi, Mohamed; Tattevin, Pierre; Donnio, Pierre-Yves; Felden, Brice // Emerging Infectious Diseases;Sep2016, Vol. 22 Issue 9, p1570 

    Staphylococcus aureus is a commensal bacterium and pathogen. Identifying biomarkers for the transition from colonization to disease caused by this organism would be useful. Several S. aureus small RNAs (sRNAs) regulate virulence. We investigated presence and expression of 8 sRNAs in 83 S. aureus...

  • Free-living Canada geese and antimicrobial resistance. Cole, Dana; Drum, David J. V.; Stallknecht, David E.; White, David G.; Lee, Margie D.; Ayers, Sherry; Sobsey, Mark; Maurer, John J.; Stalknecht, David E // Emerging Infectious Diseases;Jun2005, Vol. 11 Issue 6, p935 

    We describe antimicrobial resistance among Escherichia coli isolated from free-living Canada Geese in Georgia and North Carolina (USA). Resistance patterns are compared to those reported by the National Antimicrobial Resistance Monitoring System. Canada Geese may be vectors of antimicrobial...

  • Dihydropteroate synthase gene mutations in Pneumocystis and sulfa resistance. Huang, Laurence; Crothers, Kristina; Atzori, Chiara; Benfield, Thomas; Miller, Robert; Rabodonirina, Meja; Helweg-Larsen, Jannik // Emerging Infectious Diseases;Oct2004, Vol. 10 Issue 10, p1721 

    Pneumocystis pneumonia (PCP) remains a major cause of illness and death in HIV-infected persons. Sulfa drugs, trimethoprim-sulfamethoxazole (TMP-SMX) and dapsone are mainstays of PCP treatment and prophylaxis. While prophylaxis has reduced the incidence of PCP, its use has raised concerns about...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics