TITLE

Prion infectivity in variant Creutzfeldt-Jakob disease rectum

AUTHOR(S)
Wadsworth, J. D. F.; Joiner, S.; Fox, K.; Linehan, J. M.; Desbruslais, M.; Brandner, S.; Asante, E. A.; Collinge, J.
PUB. DATE
January 2007
SOURCE
Gut;Jan2007, Vol. 56 Issue 1, p90
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Disease-related prion protein (PrPSc) is readily detectable in lymphoreticular tissues in variant Creutzfeldt-Jakob disease (vCJD), but not in other forms of human prion disease. This distinctive pathogenesis, with the unknown population prevalence of asymptomatic vCJD infection, has led to significant concerns that secondary transmission of vCJD prions will occur through a wide range of surgical procedures. To date PrPSC:prion infectivity ratios have not been determined in vCJD, and it is unknown whether vCJD prions are similar to experimental rodent prions, where PrPSc concentration typically reflects infectious prion titre. Aim: To investigate prion infectivity in vCJD tissue containing barely detectable levels of PrPSc. Methods: Transgenic mice expressing only human PrPo/o (Tg(HuPrP129M+/+ Prnpo/o)-35 and Tg(HuPrP129M+/+ Prnp°)-45 mice) were inoculated with brain or rectal tissue from a previously characterised patient with vC.JD. These tissues contain the maximum and minimum levels of detectable PrPSc that have been observed in vCJD. Results: Efficient transmission of prion infection was observed in transgenic mice inoculated with vCJD rectal tissue containing PrPSc at a concentration of 1047-fold lower than that in vCJD brain. Conclusions: These data confirm the potential risks for secondary transmission of vCJD prions via gastrointestinal procedures and support the use of PrPSc as a quantitative marker of prion infectivity in vCJD tissues.
ACCESSION #
23627625

 

Related Articles

  • No Major Change in vCJD Agent Strain after Secondary Transmission via Blood Transfusion. Bishop, Matthew T.; Ritchie, Diane L.; Will, Robert G.; Ironside, James W.; Head, Mark W.; Thomson, Val; Bruce, Moira; Manson, Jean C. // PLoS ONE;2008, Vol. 3 Issue 8, p1 

    Background: The identification of transmission of variant Creutzfeldt-Jakob disease (vCJD) by blood transfusion has prompted investigation to establish whether there has been any alteration in the vCJD agent following this route of secondary transmission. Any increase in virulence or host...

  • Abnormal prion protein in the retina of the most commonly occurring subtype of sporadic Creutzfeldt-Jakob disease. Head, M. W.; Peden, A. H.; Yull, H. M.; Ritchie, D. L.; Bonshek, R. E.; Tullo, A. B.; Ironside, J. W. // British Journal of Ophthalmology;Sep2005, Vol. 89 Issue 9, p1131 

    Background: Involvement of the eye has been reported in patients with variant Creutzfeldt-Jakob disease (vCJD), but there is disagreement on whether retinal involvement occurs in sporadic Creutzfeldt-Jakob disease (sCJD). Methods: Western blotting, paraffin embedded tissue blotting, and...

  • Decontamination and CJD: the latest guidance. Jeffries, Don // Journal of Perioperative Practice;Nov2006, Vol. 16 Issue 11, p555 

    Professor Don Jeffries, Chair of the Advisory Committee on Dangerous Pathogens Working Group on Transmissible Spongiform Encephalopathies (ACDP TSE), Deputy Chair of the Creutzfeldt-Jakob Disease (CID) Incidents Panel, and Chair of the Department of Health Decontamination Research Group...

  • Higher Numbers than previously predicted could be incubating vCJD. Kmietowicz, Zosia // BMJ: British Medical Journal (International Edition);5/29/2004, Vol. 328 Issue 7451, p1279 

    Reports that more people in the United Kingdom than previously thought could be incubating the variant Creutzfeldt-Jakob disease. Description of the disease; Number of people that could be carrying markers for the disease.

  • Alpha-synuclein-immunoreactive deposits in human and animal prion diseases. Haïk, S.; Privat, N.; Adjou, K. T.; Sazdovitch, V.; Dormont, D.; Duyckaerts, C.; Hauw, J. J. // Acta Neuropathologica;May2002, Vol. 103 Issue 5, p516 

    Prion related disorders are associated with the accumulation of a misfolded isoform (PrPsc) of the host-encoded prion protein, PrP. There is strong evidence for the involvement of unidentified co-factors in the PrP to PrPsc conversion process. In this study, we show α-synuclein-immunoreactive...

  • Pruritus in familial Creutzfeldt-Jakob disease: a common symptom associated with central nervous system pathology. Cohen, Oren s.; Chapman, Joab; Lee, Hedok; Nitsan, Zeev; Appel, Shmuel; Hoffman, Chen; Rosenmann, Hanna; Korczyn, Amos D.; Prohovnik, Isak // Journal of Neurology;Jan2011, Vol. 258 Issue 1, p89 

    Pruritus, a common feature of animal prion diseases such as scrapie, is rarely reported in humans with Creutzfeldt-Jakob disease (CJD), and its anatomical background is not well defined. The present study was undertaken to carry out a methodical prospective search for the prevalence of pruritus...

  • Wild game feasts and fatal degenerative neurologic illness. Hoey, John // CMAJ: Canadian Medical Association Journal;9/2/2003, Vol. 169 Issue 5, p443 

    Offers information on the chronic wasting disease (CWD), a prion disease in deer and elk. Background on Creutzfeldt-Jakob disease (CJD), a similar prion disease that results to degenerative neurologic illness; Forms of CJD; Details of several cases of CWD in men who had attended wild game...

  • Barriers to Creutzfeldt-Jakob Disease Autopsies, California. Louie, Janice K.; Gavali, Shilpa S.; Belay, Ermias D.; Trevejo, Rosalie; Hammond, Lucinda H.; Schonberger, Lawrence B.; Vulgia, Due J. // Emerging Infectious Diseases;Sep2004, Vol. 10 Issue 9, p1677 

    Creutzfeldt-Jakob disease (CJD) surveillance relies on autopsy and neuropathologic evaluation. The 1990-2000 CJD autopsy rate in California was 21%. Most neurologists were comfortable diagnosing CJD (83%), but few pathologists felt comfortable diagnosing CJD (35%) or performing autopsy (29%)....

  • A history of kuru. Alpers, Michael P. // Papua New Guinea Medical Journal;2007, Vol. 50 Issue 1/2, p10 

    Kuru is placed in its geographic and linguistic setting in the Eastern Highlands of Papua New Guinea. The epidemic of kuru has declined over the period 1957 to 2005 from more than 200 deaths a year to 1 or none. Since transmission of the kuru prion agent through the mortuary practice of...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sign out of this library

Other Topics