TITLE

Chemiluminescent detection of DNA hybridization using gold nanoparticles as labels

AUTHOR(S)
Zheng-Ping Li; Cheng-Hui Liu; Yong-Shan Fan; Xin-Rui Duan
PUB. DATE
January 2007
SOURCE
Analytical & Bioanalytical Chemistry;Jan2007, Vol. 387 Issue 2, p613
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A chemiluminescent (CL) detection method has been developed for DNA hybridization. The assay relies on a sandwich-type DNA hybridization in which gold nanoparticles modified with alkylthiol-capped oligonucleotide strands are used as probes to monitor the presence of the specific target DNA. The $$ {\text{AuCl}}^{ - }_{4} $$ , which is the dissolving product of the gold nanoparticles anchored on the DNA hybrids, serves as an analyte in the H2O2–luminol– $$ {\text{AuCl}}^{ - }_{4} $$ CL reaction for the indirect measurement of the target DNA. The combination of the remarkable sensitivity of the CL analysis with the large number of $$ {\text{AuCl}}^{ - }_{4} $$ released from each DNA hybrid allows a detection limit at levels as low as 0.1 pM of the target DNA. Moreover, with a further silver amplification step, the detection limit will be pushed down to the femtomolar domain. [MediaObject not available: see fulltext.]
ACCESSION #
23616525

 

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