Stopped-flow spectrophotometric determination of nM level of Pb(II) using 5,10,15,20-terakis(1-methylpyridinium-4-yl)porphine

Kawamura, Kunio; Igarashi, Shukuro; Yotsuyanagi, Takao
February 2007
Microchimica Acta;Feb2007, Vol. 157 Issue 1/2, p87
Academic Journal
A spectrophotometric determination method for nM levels of Pb(II) has been developed using a stopped-flow spectrophotometer with cationic water-soluble porphyrin. The stopped-flow spectrophotometer allows the monitoring of the incorporation reaction of the Pb(II) ion into 5,10,15,20-terakis(1-methylpyridinium-4-yl)porphine (TMPYP) within a narrow time window (2.5 s) at pH 10.5–12.0 before the Pb-TMPYP complex is replaced with other foreign metal ions, since usually the Pb-TMPYP complex is very labile and readily replaced with other metal ions. This improved the selectivity of Pb(II) determination as compared to the conventional spectrophotometric determination of Pb(II) using water-soluble porphyrins.


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