Single-molecule tracking in eukaryotic cell nuclei

Siebrasse, Jan Peter; Grünwald, David; Kubitscheck, Ulrich
January 2007
Analytical & Bioanalytical Chemistry;Jan2007, Vol. 387 Issue 1, p41
Academic Journal
The article presents the study of single-molecule tracking in eukaryotic cell nuclei. Imaging with high resolution allows the tracing of single molecules but the concentration should be very low to avoid mixup with other molecules nearby. The study of a single cell provides a better idea than study of number of cells together. The physiological processes thus examined are very less disturbed.


Related Articles

  • Schizosaccharomyces pombe, unlike Saccharomyces cerevisiae, may not directly regulate nuclear-cytoplasmic transport of spliced tRNAs in response to nutrient availability. Pierce, Jacqueline B.; Mangroo, Dev // Biochemistry & Cell Biology;Dec2011, Vol. 89 Issue 6, p554 

    Eukaryotic cells adapt to changes in nutrient levels by regulating key processes, such as gene transcription, ribosome biogenesis, and protein translation. Several studies have shown that nuclear export of tRNAs is also regulated in Saccharomyces cerevisiae and rat hepatoma H4IIE cells during...

  • The Prp19 Complex Directly Functions in Mitotic Spindle Assembly. Hofmann, Jennifer C.; Tegha-Dunghu, Justus; Dräger, Stefanie; Will, Cindy L.; Lührmann, Reinhard; Gruss, Oliver J. // PLoS ONE;Sep2013, Vol. 8 Issue 9, p1 

    The conserved Prp19 (pre-RNA processing 19) complex is required for pre-mRNA splicing in eukaryotic nuclei. Recent RNAi screens indicated that knockdown of Prp19 complex subunits strongly delays cell proliferation. Here we show that knockdown of the smallest subunit, BCAS2/Spf27, destabilizes...

  • Alternative splicing: a pivotal step between eukaryotic transcription and translation. Kornblihtt, Alberto R.; Schor, Ignacio E.; Alló, Mariano; Dujardin, Gwendal; Petrillo, Ezequiel; Muñoz, Manuel J. // Nature Reviews Molecular Cell Biology;May2013, Vol. 14 Issue 5, p306 

    A correction to the article "Alternative splicing: a pivotal step between eukaryotic transcription and translation" by Ignacio E. Schor and colleagues is presented.

  • The dynamics of a pre-mRNA splicing factor in living cells. Misteli, Tom; Caceres, Javier F. // Nature;5/29/1997, Vol. 387 Issue 6632, p523 

    Reports the results of an investigation in the dynamic properties of pre-mRNA splicing factors in nuclei of living cells. The demonstration that speckles are highly dynamic structures that respond specifically to activation of nearby genes; The function of supplying splicing factors to active...

  • Structural basis for synaptic adhesion mediated by neuroligin-neurexin interactions. Xiaoyan Chen; Heli Liu; Shim, Ann H. R.; Focia, Pamela J.; Xiaolin He // Nature Structural & Molecular Biology;Jan2008, Vol. 15 Issue 1, p50 

    The heterophilic synaptic adhesion molecules neuroligins and neurexins are essential for establishing and maintaining neuronal circuits by modulating the formation and maturation of synapses. The neuroligin-neurexin adhesion is Ca2+-dependent and regulated by alternative splicing. We report a...

  • Successful COG8 and PDF overlap is mediated by alterations in splicing and polyadenylation signals. Pereira-Castro, Isabel; Quental, Rita; Costa, Luís; Amorim, António; Azevedo, Luisa // Human Genetics;Feb2012, Vol. 131 Issue 2, p265 

    Although gene-free areas compose the great majority of eukaryotic genomes, a significant fraction of genes overlaps, i.e., unique nucleotide sequences are part of more than one transcription unit. In this work, the evolutionary history and origin of a same-strand gene overlap is dissected...

  • Localization of pre-mRNA splicing in mammalian nuclei. Zhang, Guohong; Taneja, Krishan L. // Nature;12/22/1994, Vol. 372 Issue 6508, p809 

    Investigates the localization of precursor messenger RNA splicing in mammalian nuclei. Distribution of precursor messenger RNA splicing factors; Presence of nuclear `compartments' that are distinct from the sites of transcription; Localization of nascent spliced messenger RNA.

  • tRNA—mRNA mimicry drives translation initiation from a viral IRES. Costantino, David A.; Pfingsten, Jennifer S.; Rambo, Robert P.; Kieft, Jeffrey S. // Nature Structural & Molecular Biology;Jan2008, Vol. 15 Issue 1, p57 

    Internal ribosome entry site (IRES) RNAs initiate protein synthesis in eukaryotic cells by a noncanonical cap-independent mechanism. IRESes are critical for many pathogenic viruses, but efforts to understand their function are complicated by the diversity of IRES sequences as well as by limited...

  • Crystal structure of SopA, a Salmonella effector protein mimicking a eukaryotic ubiquitin ligase. Jianbo Diao; Ying Zhang; Huibregtse, Jon M.; Daoguo Zhou; Jue Chen // Nature Structural & Molecular Biology;Jan2008, Vol. 15 Issue 1, p65 

    Bacterial pathogens deliver virulence proteins into host cells to facilitate entry and survival. Salmonella SopA functions as an E3 ligase to manipulate the host proinflammatory response. Here we report the crystal structure of SopA in two conformations. Although it has little sequence...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics