TITLE

Microanalyzer for biomonitoring lead (Pb) in blood and urine

AUTHOR(S)
Yantasee, Wassana; Timchalk, Charles; Lin, Yuehe
PUB. DATE
January 2007
SOURCE
Analytical & Bioanalytical Chemistry;Jan2007, Vol. 387 Issue 1, p335
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Biomonitoring of lead (Pb) in blood and urine enables quantitative evaluation of human occupational and environmental exposures to Pb. State-of-the-art ICP–MS instruments can only analyze metals in laboratories, resulting in lengthy turnaround times, and they are expensive. In response to the growing need for a metal analyzer capable of on-site, real-time monitoring of trace toxic metals in individuals, we developed a portable microanalyzer based on flow-injection/stripping voltammetry (ASV), and validated the system using rat blood and urine spiked with known amounts of Pb. Fouling of electrodes by proteins often prevents the effective use of electrochemical sensors in biological matrices. Minimization of such fouling was accomplished with suitable sample pretreatment and by establishing turbulent flow of blood and urine containing Pb onto the electrode inside the microanalyzer, which resulted in no apparent electrode fouling even when the samples contained 50% urine or 10% blood by volume. No matrix effect was observed for the voltammetric Pb signals, even when the samples contained 10% blood or 10% urine. The microanalyzer offered linear concentration ranges relevant to Pb exposure levels in humans (0–20 ppb in 10% blood samples, 0–50 ppb in 50% urine samples). The device showed excellent sensitivity and reproducibility; Pb detection limits were 0.44 ppb and 0.46 ppb, and % R.S.D. was 4.9 and 2.4 in 50% urine and 10% blood samples, respectively. It gave similar Pb concentrations in blood and urine to those measured by ICP–MS. It offered high throughput (3 min per sample) and economical use of samples (60 μL per measurement) as well as low reagent consumption (1 μg of Hg per measurement), thus minimizing environmental concerns associated with mercury use. Since it is miniaturized, the microanalyzer is portable and field-deployable. Thus, it shows much promise as the next-generation analyzer for the biomonitoring of toxic metals.
ACCESSION #
23460802

 

Related Articles

  • Combination of gel electrophoresis and ICP-mass spectrometry–novel strategies for phosphoprotein measurement. Elliott, Victoria L.; McLeod, Cameron W.; Marshall, Peter S. // Analytical & Bioanalytical Chemistry;Oct2005, Vol. 383 Issue 3, p416 

    The potential for developing improved procedures for phosphate measurement through combinations of gel electrophoresis and quadrupole-based ICP mass spectrometry utilising 47PO+ is investigated. Laser ablation of gels offers a rapid and direct quantitation route, but is subject to high blanks...

  • Continuous online determination of 226Ra in liquid effluents using automated column chromatography-ICP-MS. Evans, R. Douglas; Izmer, Andrei; Benkhedda, Karima; Toms, Andrew; Fernando, Angelo; Wang, Wei // Canadian Journal of Chemistry;Nov2015, Vol. 93 Issue 11, p1226 

    A measurement system capable of continuous on-line matrix removal, pre-concentration and analysis of 226Ra using pre-packed columns coupled to a flow injection system and an ICP-MS was developed. Full instrumental control of both the ICP-MS and the flow injection system provided automatic...

  • Automated Flow Injection Coupled with ICP-MS for the Online Determination of Trace Silver in Seawater. Zuhao Zhu; Airong Zheng // Spectroscopy;Jan2017, Vol. 32 Issue 1, p50 

    This article reports a sensitive analytical procedure for the analysis of silver (Ag) in seawater, using a simple automated flow injection system coupled online with inductively coupled plasma-mass spectrometry (ICP-MS). Parameters including flow rate and duration, and the effects of the pH and...

  • Urinary iodine concentrations of pregnant women in rural Bangladesh: A longitudinal study. Rydbeck, Filip; Bottai, Matteo; Tofail, Fahmida; Persson, Lars-Åke; Kippler, Maria // Journal of Exposure Science & Environmental Epidemiology;Sep/Oct2014, Vol. 24 Issue 5, p504 

    Iodine is an essential dietary element required for normal fetal growth and development. We aimed to explore intraindividual and interindividual variations in iodine intake in pregnant women and whether non-dietary factors might influence the intake. Iodine intake was assessed in 271 women,...

  • Trace element concentrations from lichen transplants in Pretoria, South Africa. Olowoyo, Joshua Oluwole; van Heerden, E.; Fischer, J. L. // Environmental Science & Pollution Research;May2011, Vol. 18 Issue 4, p663 

    Purpose: The purpose of this study was to assess the level and possible sources of trace elements in Tshwane metropolis using transplanted lichen thallus of Parmelia sulcata with a view to evaluating the ability of this lichen species to monitor air pollutants from a perceived polluted...

  • Toxic metal levels in children residing in a smelting craft village in Vietnam: a pilot biomonitoring study. Sanders, Alison P.; Miller, Sloane K.; Viet Nguyen; Kotch, Jonathan B.; Fry, Rebecca C. // BMC Public Health;2014, Vol. 14 Issue 1, p1 

    Background In Vietnam, environmental pollution caused by small-scale domestic smelting of automobile batteries into lead ingot is a growing concern. The village of Nghia Lo is a smelting craft village located roughly 25 km southeast of Hanoi in the Red River Delta. Despite the concern of toxic...

  • Assessment of Atmospheric Metal Pollution in the Urban Area of Mexico City, Using Ficus benjamina as Biomonitor. Guzmán-Morales, Janin; Morton-Bermea, Ofelia; Hernández-Álvarez, Elizabeth; Rodríguez-Salazar, María; García-Arreola, María; Tapia-Cruz, Víctor // Bulletin of Environmental Contamination & Toxicology;May2011, Vol. 86 Issue 5, p495 

    Concentrations of vanadium, chromium, cobalt, nickel, copper, zinc, antimony, and lead were measured in Ficus benjamina leaves from the Mexico City urban area in order to assess their enrichment against background values. The instrumental analysis was performed using inductively coupled plasma...

  • Response of air-pollution biomonitors under three different meteorological conditions. Canha, N.; Freitas, M.; Pacheco, A. // Journal of Radioanalytical & Nuclear Chemistry;Jan2013, Vol. 295 Issue 1, p489 

    Samples of Flavoparmelia caperata (L.) Hale thalli, from a clean area in northern Portugal (Baião), were transplanted into exposure locations at the western Atlantic coast (north, center and south) impacted by urban-industrial emissions and meteorologically different, for a ten-month long...

  • Time-resolved monitoring of heavy-metal intoxication in single hair by laser ablation ICP—DRCMS. Stadlbauer, Christina; Prohaska, Thomas; Reiter, Christian; Knaus, Anna; Stingeder, Gerhard // Analytical & Bioanalytical Chemistry;Oct2005, Vol. 383 Issue 3, p500 

    The potential of laser ablation inductively coupled plasma mass spectrometry for the time-resolved analysis of heavy-metal intoxication in human bodies by analysis of hair is demonstrated. As application, we analyzed forensic samples from one individual after Hg intake and from one treated with...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics