Distribution of SPARC during neovascularisation of degenerative aortic stenosis

Charest, A.; Pépin, A.; Shetty, R.; Côté, C.; Voisine, P.; Dagenais, F.; Pibarot, P.; Mathieu, P.
December 2006
Heart;Dec2006, Vol. 92 Issue 12, p1844
Academic Journal
Objective: To examine the hypothesis that degenerative aortic stenosis (AS) is associated with the development of blood vessels and the expression of the secreted protein, acidic and rich in cysteine/ osteonectin (SPARC), a matricellular protein that is involved in ossification, the modulation of angiogenesis and the production of metalloproteinases. Methods: 30 surgically excised AS valves and 20 normal aortic valves were studied. Results: Blood vessels were detected in the aortic valves from patients with degenerative AS, whereas normal valves were avascular structures. Blood vessels in AS valves expressed endothelial nitric oxide synthase, CD34 and von Willebrand factor (vWF). Blood vessels were located in three distinct regions: near calcified nodules, under the leaflet border and in rich cellular areas forming cell islands. Blood vessels were predominantly present in early and intermediate grades of calcification. Cell islands were densely populated by CD45-positive cells where endothelial cells (CD34+, vWF+) forming cord-like structures were present. lmmunoblotting detected SPARC only in AS valves and immunohistological analysis located SPARC in mature blood vessels. The proportion of blood vessels positive for SPARC was higher in valves with a lower grade of calcification. In cell islands, SPARC was distributed to mature blood vessels and to macrophages, where it co-located with matrix metalloproteinase-9, whereas no expression was detected in endothelial cells forming cord-like structures. Conclusion: The localisation of SPARC to mature blood vessels and its predominant expression in AS valves with a lower calcification grade suggest that the spatial and temporal distribution of this matricellular protein is tightly controlled to participate in the neovascularisation of AS valves.


Related Articles

  • Diffuse Supravalvular Aortic Stenosis with Multiple Stenoses of the Branches of Arcus Aorta in a Child. Uçar, Tayfun; Tutar, Ercan; Atalay, Semra // Pediatric Cardiology;Jan2008, Vol. 29 Issue 1, p228 

    We give details of a sporadic case with congenital supravalvular aortic stenosis associated with critical stenosis of the left carotid artery, and severe stenosis of the innominate artery at their origins as well as excessive dilatations of both the right and the left coronary arteries.

  • Rheology of discrete subaortic stenosis. Cilliers, A.M.; Gewillig, M. // Heart;Oct2002, Vol. 88 Issue 4, p335 

    Discusses the rheology of discrete subaortic stenosis, which may be a combination of factors such as an underlying genetic predisposition, turbulence in the left ventricular outflow tract and various genetic and anatomical variations of the left ventricular outflow tract. Anatomical sites and...

  • Inflammation Is Associated with the Remodeling of Calcific Aortic Valve Disease. Coté, Nancy; Mahmut, Ablajan; Bosse, Yohan; Couture, Christian; Pagé, Sylvain; Trahan, Sylvain; Boulanger, Marie-Chloé; Fournier, Dominique; Pibarot, Philippe; Mathieu, Patrick // Inflammation;Jun2013, Vol. 36 Issue 3, p573 

    Calcific aortic valve disease (CAVD) is the most frequent heart valve disorder. Studies indicate that mineralization of the aortic valve may be related to the inflammatory process. However, no clear evidence has been given regarding clinical evolution of aortic stenosis and the inflammatory...

  • Transfemoral transcatheter aortic valve implantation in a patient with multiple endovascular aortic stents--a case report. Weber, Carolyn; Deppe, Antje-Christin; Eghbalzadeh, Kaveh; Scherner, Maximilian; Gray, Daphne; Majd, Payman; Gawenda, Michael; Rosenkranz, Stephan; Rudolph, Tanja; Madershahian, Navid; Wahlers, Thorsten // Journal of Cardiothoracic Surgery;2/2/2016, Vol. 11, p1 

    Background: In patients undergoing transfemoral transcatheter aortic valve implantation, previous endovascular interventions bear a risk for the valve frame to get stucked to the aortic stents.Case Presentation: We report on a 75-year-old frail patient with severe...

  • Angiogenesis in Fatal Acute Kawasaki Disease Coronary Artery and Myocardium. Freeman, A. F.; Crawford, S. E.; Cornwall, M. L.; Garcia, F. L.; Shulman, S. T.; Rowley, A. H. // Pediatric Cardiology;Sep/Oct2005, Vol. 26 Issue 5, p578 

    Angiogenesis has been shown to be dysregulated in coronary artery (CA) aneurysms in the chronic phase of Kawasaki disease (KD). Neovascularization may occur in inflammatory-related vascular diseases because many angiogenesis mediators are secreted by inflammatory cells. We hypothesized that...

  • Disturbed angiogenesis in systemic sclerosis: high levels of soluble endoglin. J. Wipff; J. Avouac; D. Borderie; D. Zerkak; H. Lemarechal; A. Kahan; C. Boileau; Y. Allanore // Rheumatology;Jul2008, Vol. 47 Issue 7, p972 

    Objective. SSc is a CTD characterized by early generalized microangiopathy with disturbed angiogenesis. Soluble endoglin (sENG), a serum anti-angiogenic protein, has recently been described as a major actor in pre-eclampsia, another severe vascular disease with abnormal angiogenesis. The aim of...

  • Alternative access shows promise in extreme-risk patients treated with CoreValve.  // Cardiology Today;Jun2014, Vol. 17 Issue 6, p26 

    The article presents a medical report which suggests that treatment with CoreValve System through alternative access routes proved to be beneficial for extreme-risk patients with sever aortic stenosis.

  • Calcific aortic stenosis: from bench to the bedside--emerging clinical and cellular concepts. Rajamannan, Nalini M.; Gersh, Bernard; Bonow, Robert O. // Heart;Jul2003, Vol. 89 Issue 7, p801 

    Describes calcific aortic stenosis as the cause of aortic valve disease in developed countries. Increase in the prevalence of the disease; Cellular basis of calcific aortic stenosis; Occurrence of the disease in the left ventricular hypertrophy.

  • Obstruction to Left Ventricular Outflow. Morrow, Andrew g.; Roberts, William C.; Ross Jr., John; Fisher, Darryl; Behrendt, Douglas M.; Mason, Dean T.; Braunwald, Eugene // Annals of Internal Medicine;Dec68, Vol. 69 Issue 6, p1255 

    Discusses the concepts of management and operative treatment of obstruction to left ventricular outflow. Pathologic anatomy of the stenotic aortic valve; Natural history of severe aortic stenosis in adults; Complications and deaths associated with aortic valve replacement.


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics