Composition and catalase-like activity of Mn(II)-bicarbonate complexes

Tikhonov, K. G.; Zastrizhnaya, O. M.; Kozlov, Yu. N.; Klimov, V. V.
November 2006
Biochemistry (00062979);Nov2006, Vol. 71 Issue 11, p1270
Academic Journal
The composition and catalase-like activity of Mn2+ complexes with bicarbonate were investigated with voltammetry and kinetic methods (by the rate of O2 production from H2O2). Three linear sections were revealed on the dependence of the reduction potential of Mn2+ on logarithm of bicarbonate concentration ( $$\log C_{NaHCO_3 } $$ ) having slopes equal to 0 mV/ $$\log C_{NaHCO_3 } $$ , −14 mV/ $$\log C_{NaHCO_3 } $$ , and −59 mV/ $$\log C_{NaHCO_3 } $$ , corresponding to Mn2+ aqua complex (Mn) and to Mn2+-bicarbonate complexes of the composition [Mn2+(HCO)]+ (at concentration of HCO 10–100 mM) and [Mn2+(HCO)2]0 (at concentration of HCO 100–600 mM). Comparison of HCO concentration needed for the catalase-like activity of Mn2+ with the electrochemical data showed that only electroneutral complex Mn2+(HCO)2 catalyzed decomposition of H2O2, whereas positively charged Mn complex with [Mn2+(HCO)]+ were not active. The catalase-like activity of Mn2+ did not appear upon substitution of anions of carbonic acids (acetate and formate) for HCO. The rate of O2 production in the system Mn2+-HCO-H2O2 (pH 7.4) is proportional to the second power of Mn2+ concentration and to the fourth power of HCO concentration that indicates simultaneous involvement of two Mn2+(HCO)2 complexes in the reaction of H2O2 decomposition.


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