TITLE

Effect of extract of Phyllanthus niruri on crystal deposition in experimental urolithiasis

AUTHOR(S)
Barros, Marcio E.; Lima, Roberta; Mercuri, Lucildes P.; Matos, Jivaldo R.; Schor, Nestor; Boim, Mirian A.
PUB. DATE
December 2006
SOURCE
Urological Research;Dec2006, Vol. 34 Issue 6, p351
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Phyllanthus niruri (Pn) is a plant that has been shown to interfere in the growth and aggregation of calcium oxalate (CaOx) crystals. In the present study we evaluated the effect of Pn on the preformed calculus induced by introduction of a CaOx seed into the bladder of male Wistar rats. Pn treatment (5 mg/rat/day) was initiated immediately or 30 days after CaOx seeding and thus in the presence of a preformed calculus. Animals were sacrificed 50 or 70 days after surgery. The resulting calculi were weighed and analyzed by X-ray diffraction, stereomicroscopy and scanning electronic microscopy. Precocious Pn treatment reduced the number (75%, P < 0.05) and the weight (65%, P < 0.05) of calculi that frequently exhibited a matrix-like material on its surface, compared to the untreated CaOx group. In contrast, Pn treatment in the presence of a preformed calculus did not prevent further calculus growth; rather, it caused an impressive modification in its appearance and texture. Calculi from Pn-treated animals had a smoother, homogeneous surface compared to the spicule shape of calculi found in the untreated CaOx group. XRD analysis revealed the precipitation of struvite crystals over the CaOx seed and Pn did not change the crystalline composition of the calculi. This suggests that Pn interfered with the arrangement of the precipitating crystals, probably by modifying the crystal�crystal and/or crystal�matrix interactions. Results suggest that Pn may have a therapeutic potential, since it was able to modify the shape and texture of calculi to a smoother and probably more fragile form, which could contribute to elimination and/or dissolution of calculi.
ACCESSION #
23260922

 

Related Articles

  • Determination of the chemical composition of urinary calculi by noncontrast spiral computerized tomography. Sheir, Khaled Z.; Mansour, Osama; Madbouly, Khaled; Elsobky, Emad; Abdel-Khalek, Mohamed // Urological Research;May2005, Vol. 33 Issue 2, p99 

    Various techniques for noncontrast spiral computerized tomography (NCCT) were utilized for the determination of the Hounsefield unit (HU) values of various types of urinary calculi with the aim of determining the best technique for distinguishing various stones compositions. A total of 130...

  • Evaluation of antiurolithic effect and the possible mechanisms of Desmodium styracifolium and Pyrrosiae petiolosa in rats. Mi, Jun; Duan, Jianmin; Zhang, Jun; Lu, Jianzhong; Wang, Hanzhang; Wang, Zhiping // Urological Research;Apr2012, Vol. 40 Issue 2, p151 

    Chinese herbs Desmodium styracifolium (Ds) and Pyrrosiae petiolosa (Pp) have been widely used to treat urolithiasis with few side effects in traditional Chinese medicine (TCM). In vitro crystallization study has confirmed their prophylaxis of Calcium oxalate (CaO) stones formation. However,...

  • Inhibition of calcium oxalate crystal deposition on kidneys of urolithiatic rats by Hibiscus sabdariffa L. extract. Laikangbam, Reena; Damayanti Devi, M. // Urological Research;Jun2012, Vol. 40 Issue 3, p211 

    The present study aims at systematic evaluation of the calyces of Hibiscus sabdariffa to establish its scientific validity for anti-urolithiatic property using ethylene glycol-induced hyperoxaluria model in male albino rats. Administration of a mixture of 0.75% ethylene glycol and 2% ammonium...

  • The effect of traditional risk factors for stone disease on calcium oxalate crystal adherence in the rat bladder. Smith, Charles; St Peter, John // Urological Research;Oct2007, Vol. 35 Issue 5, p243 

    Crystal adherence in the urinary tract has been studied using the chemically injured rat bladder and cell cultures. These studies have provided evidence that mucin prevents adherence and have studied various compounds for their ability to promote or inhibit crystal adherence. Little work has...

  • Improved methodology to induce hyperoxaluria without treatment using hydroxyproline. Wiessner, John; Garrett, Michael; Hung, Linda; Wille, David; Mandel, Neil // Urological Research;Oct2011, Vol. 39 Issue 5, p373 

    The use of hydroxyproline (HP) to generate hyperoxaluria in the rat is a problem because it is impossible to separate the effect of oxalate on renal injury from the effects of HP and the large array of metabolic intermediates formed when HP is converted to oxalate. Previously, the Dahl...

  • Thermal analysis of kidney stones and their characterization. Kohutova, A.; Honcova, P.; Podzemna, V.; Bezdicka, P.; Vecernikova, E.; Louda, M.; Seidel, J. // Journal of Thermal Analysis & Calorimetry;Aug2010, Vol. 101 Issue 2, p695 

    Thermal decomposition and structural characterization of three human kidney stones (KS1–KS3) extracted from patients of Eastern Bohemia have been carried out using X-ray powder diffraction systems (XRD), scanning electron microscope with energy dispersive X-ray micro analyser (SEM-EDX)...

  • RESEARCH COMMUNICATIONS: Microstructure and growth band studies of uroliths using optical and scanning electron microscopy. Kale, Sanjay S.; Pawar, N. J.; Wagholikar, D. S.; Achyuthan, Hema // Current Science (00113891);1/25/2011, Vol. 100 Issue 2, p225 

    Detailed description, analysis and interpretation of bio-mineral formations are fundamental to an understanding of their growth and origin. Study of morphology at the macro-level can be supplemented by examining the specimen under the microscope using thin sections prepared from undisturbed...

  • Stone Morphology: Implication for Pathogenesis. Daudon, Michel; Jungers, Paul; Bazin, Dominique // AIP Conference Proceedings;9/18/2008, Vol. 1049 Issue 1, p199 

    Urinary stones of similar crystalline composition as identified by X-ray diffraction or Fourier transform infrared spectroscopy (FT-IR) may exhibit distinct structures, which are reflected in distinctive stone morphology. Among factors involved in stone morphology—some reflecting...

  • Prevention of renal crystal deposition by an extract of Ammi visnaga L. and its constituents khellin and visnagin in hyperoxaluric rats. Vanachayangkul, P.; Chow, N.; Khan, S.; Butterweck, Veronika // Urological Research;Jun2011, Vol. 39 Issue 3, p189 

    In Egypt, teas prepared from the fruits of Ammi visnaga L. (syn. 'Khella') are traditionally used by patients with urolithiasis. The aim of this study was to evaluate whether oral administration of an aqueous extract prepared from the fruits of A. visnaga as well as two major constituents...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics