TITLE

Effects of ageing on spermatozoal chromatin and its sensitivity to in vivo and in vitro oxidative challenge in the Brown Norway rat

AUTHOR(S)
Ekaterina V. Zubkova; Bernard Robaire
PUB. DATE
November 2006
SOURCE
Human Reproduction;Nov2006, Vol. 21 Issue 11, p2901
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
BACKGROUND: The goals of our study were to examine chromatin packaging and integrity in spermatozoa taken from the caput and cauda epididymides of young (4-month-old) and old (21-month-old) Brown Norway rats and to assess whether spermatozoal sensitivity to oxidative treatments is altered with age. METHODS: Oxidative treatments consisted of (i) in vivo oxidative challenge by systemic administration of the glutathione-depleting drug l‐buthionine-[S,R]-sulphoximine (BSO) and (ii) in vitro oxidative challenge by incubating collected spermatozoa with hydrogen peroxide (H2O2). Chromatin parameters assessed included quantification of thiols, nuclear chromomycin A3 (CMA3) penetration, DNA breaks by TdT (terminal deoxynucleotidyl transferase)-mediated dUDP nick-end labelling (TUNEL) and ease of DNA dissociation by acridine orange (AO) staining. RESULTS: In spermatozoa from older rats, we found decreases in thiols, CMA3 penetration and the percentage of cells that undergo DNA dissociation. Administration of BSO had oxidizing effects on the thiol groups. It also decreased CMA3 penetration and DNA dissociation and increased TUNEL staining. Furthermore, BSO treatment sensitized cauda epididymidis spermatozoa, from older animals, to H2O2. CONCLUSIONS: Overall, we show that spermatozoa from older rats have altered chromatin packaging and integrity and that spermatozoa from the cauda epididymidis are more responsive to combined in vivo and in vitro oxidative challenge than spermatozoa from young rats.
ACCESSION #
23260492

 

Related Articles

  • Exposure of male rats to cyclophosphamide alters the chromatin structure and basic proteome in spermatozoa. A.M. Codrington; B.F. Hales; B. Robaire // Human Reproduction;May2007, Vol. 22 Issue 5, p1431 

    : BACKGROUND The formation of mature sperm involves the expression of numerous proteins during spermiogenesis and the replacement of histones with protamines to package the genome. Exposure to cyclophosphamide (CPA), an anticancer alkylating agent, during spermiogenesis may disrupt chromatin...

  • Evaluation of Cryoinjury of Spermatozoa After Slow (Programmed Biological Freezer) or Rapid (Liquid Nitrogen Vapour) Freeze--Thawing Techniques. Hammadeh, M.E.; Szarvasy, D.; Zeginiadou, T.; Rosenbaum, P.; Georg, T.; Schmidt, W. // Journal of Assisted Reproduction & Genetics;Jul2001, Vol. 18 Issue 7, p364 

    Investigates the occurrence of cryoinjury of spermatozoa following freeze-thawing techniques. Use of acridine orange in assessing chromatin cryoinjury; Detrimental effect of freeze-thawing on sperm; Estimation of the chromatin deterioration rates.

  • Packaging paternal chromosomes with protamine. Braun, Robert E. // Nature Genetics;May2001, Vol. 28 Issue 1, p10 

    Studies the role of protamine genes in sperm chromatin remodeling. Intermediaries in the histone-to-protamine transition; Chromatin packaging in somatic cells; Nuclear condensation of protamines; Relationship between chromatin repackaging in sperm and developmental reprogramming.

  • Seasonal Changes in Human Sperm Chromatin Condensation. Henkel, Ralf; Menkveld, Roelof; Kleinhappl, Marion; Schill, Wolf-Bernard // Journal of Assisted Reproduction & Genetics;Jul2001, Vol. 18 Issue 7, p371 

    Examines the influence of seasonal changes on human sperm count and chromatin condensation in Germany. Correlation between endometrium hyperplasia and anovulatory cycle; Role of seasonal rhythm in human reproduction; Increase in number of spermatozoa in springtime.

  • Molecular determinants of nucleosome retention at CpG-rich sequences in mouse spermatozoa. Erkek, Serap; Hisano, Mizue; Liang, Ching-Yeu; Gill, Mark; Murr, Rabih; Dieker, Jürgen; Schübeler, Dirk; van der Vlag, Johan; Stadler, Michael B; Peters, Antoine H F M // Nature Structural & Molecular Biology;Oct2013, Vol. 20 Issue 10, p1236 

    A correction to the article "Molecular determinants of nucleosome retention at CpG-rich sequences in mouse spermatozoa" that was published in the 2013 issue is presented.

  • Vitamin C attenuates detrimental effects of diabetes mellitus on sperm parameters, chromatin quality and rate of apoptosis in mice. Talebi A. R.; Mangoli E.; Nahangi H.; Anvari M.; Pourentezari M.; Halvaei I. // Iranian Journal of Reproductive Medicine;Apr2015 Supplement, p81 

    Introduction: Diabetes Mellitus (DM) may affect male reproductive functions at multiple levels. It is shown that intake of antioxidants such as vitamin C and E can reinforce the stability of testicular blood barrier and protect sperm DNA from oxidative stress.The main goal was to examine the...

  • Altered protamine expression and diminished spermatogenesis: what is the link? Douglas T. Carrell; Benjamin R. Emery; Sue Hammoud // Human Reproduction Update;May2007, Vol. 13 Issue 3, p313 

    During the elongating spermatid stage of spermiogenesis, human sperm chromatin undergoes a complex transition in which histones are extensively replaced by protamines in a carefully regulated transition including histone modifications and intermediate and temporary replacement of the histones by...

  • Effect of saffron on rat sperm chromatin integrity. Mardani, Mohammad; Vaez, Ahmad; Razavi, Shahnaz // Iranian Journal of Reproductive Medicine;May2014, Vol. 12 Issue 5, p343 

    Background: Currently, relation between reactive oxygen species (ROS) ROS concentration and semen quality was indicated. Saffron has traditionally been not only considered as a food additive but also as a medicinal herb, which has a good antioxidant properties. Objective: The aim of this study...

  • 400H-CP Inhibits Sperm Respiration.  // Fertility Weekly;2/16/2009, p10 

    The article discusses a research report which states that mesna and WR-1065, commonly known as amifostine, protect the human sperm respiration that is negatively affected by 4-hydroperoxycyclophosphamide. It is stated that 4OOH-CP is a peroxidized analog of cyclophosphamide, an anti-cancer drug....

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics