Stereological analysis of muscle morphology following exposure to repetitive stretch-shortening cycles in a rat model

Baker, Brent A.; Mercer, Robert R.; Geronilla, Ken B.; Kashon, Michael L.; Miller, G. R.; Cutlip, Robert G.
April 2006
Applied Physiology, Nutrition & Metabolism;Apr2006, Vol. 31 Issue 2, p167
Academic Journal
Repetitive motion is one risk factor associated with contraction-induced muscle injury, which leads to skeletal muscle degeneration, inflammation, and dysfunction. Since current methods are unable to quantify the acute degenerative and inflammatory responses of muscle tissue concurrently, the purpose of this study was to quantify the temporal myofiber response after exposure to injurious stretch–shortening cycles (SSCs) using a standardized stereological technique. Functional testing was performed on the ankle dorsiflexor muscles of Sprague–Dawley rats in vivo. Rats were anesthetized and exposed to 15 sets of 10 SSCs. Control rats were exposed to 15 sets of single isometric contractions of the same stimulation duration. Changes in muscle morphometry were assessed at 0.5, 24, 48, 72, and 240 h post-exposure to quantify the degree of myofiber degeneration and inflammation in the tibialis anterior muscle from each group. There was an increase in the volume density and average thickness of degenerating myofibers over time in the muscle collected from rats exposed to SSCs (p < 0.0001) that was significantly greater than in muscle exposed to isometric contractions at 24, 48, and 72 h post-exposure (p = 0.003). The volume density of degenerative myofibers was associated with functional deficits at 48 h. Stereological quantification of degenerative myofibers and interstitial space changes were associated with functional defects 48–72 h after SSC-induced injury, thus demonstrating stereology is an accurate measure of SSC-induced skeletal muscle injury.


Related Articles

  • Procysteine Increases Alcohol-depleted Glutathione Stores in Rat Plantaris Following a Period of Abstinence. Otis, Jeffrey S.; Guidot, David M. // Alcohol & Alcoholism;Nov/Dec2010, Vol. 45 Issue 6, p495 

    Aims: To assess the effectiveness of procysteine (PRO) supplementation provided during a period of abstinence (ABS) on alcohol-induced skeletal muscle atrophy and oxidant stress. Methods: Age- and gender-matched Sprague–Dawley rats were fed the Lieber–DeCarli liquid diet containing...

  • Structure and Concentration of Elastic System Fibers in the Trachea of the Rat.  // International Journal of Morphology;2011, Vol. 29 Issue 1, p16 

    No abstract available.

  • Effects of Aspartame on Fetal Kidney: A Morphometric and Stereological Study. Martins, Marielza R. Ismael; Azoubel, Reinaldo // International Journal of Morphology;2007, Vol. 25 Issue 4, p689 

    The objective of this study was the evaluation of aspartame effects on morphometric alterations of the glomerulus, proximal and distal convoluted tubules and collecting ducts of the rat fetal kidney during organogenesis. Fifteen pregnant rats averaging 24 g body weight, were divided into 3...

  • Effects of ethyl pyruvate administration on female rats' pyramidal cells of cornu ammonis after brain ischemia: a stereological and histopathological study. İKİNCİ, Ayşe; ODACI, Ersan; BAŞ, Orhan // Turkish Journal of Medical Sciences;Jun2013, Vol. 43 Issue 3, p354 

    Aim: Since ethyl pyruvate (EP) is a known neuroprotective agent, this study was intended to investigate the effects of EP on pyramidal cell numbers and morphology in the hippocampus cornu ammonis after brain ischemia. Materials and methods: Female rats were divided into 5 groups: control,...

  • End-To-Side Loop Neurorrhaphy: Axonal Comparative Stereological Study. Boer, Nagib Pezati; Viterbo, Fausto; Faleiros, Humberto Regis; Batigalia, Fernando; Ricci Boer, Ana Luísa; Carvalho Jr., Altair Antonio // International Journal of Morphology;set2014, Vol. 32 Issue 3, p942 

    Accidents or diseases can affect the peripheral part of the nervous system, which raises clinical and surgical therapies, among others. In this context, the technique of end-to-side neurorrhaphy is a treatment option, yet its modification loop needs some additional efficacy studies. The purpose...

  • PHYSIOLOGICAL ROLE OF MYOSIN LIGHT AND HEAVY CHAIN ISOFORMS IN FAST- AND SLOW-TWITCH MUSCLES: EFFECT OF EXERCISE. Alev, K.; Kaasik, P.; Pehme, A.; Aru, M.; Parring, A.-M.; Elart, A.; Seene, T. // Biology of Sport;2009, Vol. 26 Issue 3, p215 

    The aims of the present study were to show the distribution of individual myosin light chain (MyLC) isoforms in fast-twitch (FT) and slow-twitch (ST) muscles and between FT muscles in order to find differences between MyLC isoforms in these muscles, to identify similarities with the distribution...

  • Muscle fragments on a scaffold in rats: a potential regenerative strategy in urogynecology. Jangö, Hanna; Gräs, Søren; Christensen, Lise; Lose, Gunnar // International Urogynecology Journal;Dec2015, Vol. 26 Issue 12, p1843 

    Introduction and hypothesis: The use of permanent synthetic meshes to improve the outcome of pelvic organ prolapse (POP) repair causes frequent and serious complications. The use of the synthetic, biodegradable scaffold methoxypolyethyleneglycol-polylactic-co-glycolic acid (MPEG-PLGA) seeded...

  • A Procedure for Extracting Calcium-45 from Teeth. WATSON, JOSEPH A.; COLLETT, WILLIAM K. // Journal of Dental Research;Nov1964, Vol. 43 Issue 6, p1253 

    The article presents an outline for obtaining calcium-45 from teeth. The author discusses some of the problems associated with obtaining teeth and hard tissues for radioactive analysis. The process for extracting calcium-45 from rat teeth is presented and the effectiveness of the process is...

  • © IN VITRO DEVELOPMENT OF ZINC-DEFICIENT AND REPLETE RAT EMBRYOS. Record, I. R.; Dreosti, I. E.; Tulsi, R. S. // Australian Journal of Experimental Biology & Medical Science;Feb1985, Vol. 63 Issue 1, p65 

    Studies the in vitro development of zinc-deficient and replete rat embryos using embryo culture techniques. Division of the zinc-deficient embryos into two broad morphological categories; Removal of the embryos from dams which had been fed a zinc-deficient diet since mating for culture;...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics