There are two 5'-flanking regions of bkt encoding beta-carotene ketolase in Haematococcus pluvialis

Chunxiao Meng; Chengwei Liang; Zhongliang Su; Song Qin; Chengkui Tseng
March 2006
Phycologia;Mar2006, Vol. 45 Issue 2, p218
Academic Journal
The unicellular green alga Haematococcus pluvialis accumulates a commercially valuable astaxanthin, with levels reaching up to 4% dry weight under environmental stress. In recent years, much effort has been devoted to understanding the molecular mechanisms regulating astaxanthin biosynthetic pathways. Beta-carotene ketolase (bkt), with control being exhibited at the transcription level, plays an important role in astaxanthin biosynthesis by H. pluvialis. Here we demonstrate the presence of two separate 5′-flanking regions [1.5 kilobase (kb) and 2 kb] of bkt (bkt1 and bkt2) that possess regulatory elements similar to those of known stress-responsive genes in plants. Results of 5′-deletion constructs and transient beta-galactosidase expression assays demonstrate that there may be positive regulatory elements governing expression in the shorter promoter at -1060/-900 from the 1.5 kb 5′ region, and in the longer promoter at -1838/-1219 and at -1046/-734 from the 2 kb 5′ region relative to each homologous ATG start codon. Furthermore, our present studies reveal that the first intron (+371/+497) downstream from the 1.5 kb 5′ untranslated region of bkt1 may function as a negative regulatory element to regulate its own promoter.


Related Articles

  • The queer Tetraëdron minimum from Lake Kivu (Eastern Africa): is it a result of a human impact? Stoyneva, Maya; Descy, Jean-Pierre; Balagué, Vanessa; Compère, Pierre; Leitao, Maria; Sarmento, Hugo // Hydrobiologia;Nov2012, Vol. 698 Issue 1, p273 

    The coccal unicellular green algal genus Tetraëdron Kütz. ex Korshikov, which can be easily identified by its typical polygonal shape, is a common member of freshwater plankton and metaphyton, frequently observed in lowland temperate and tropical waters. During the analysis of samples from...

  • Molecular Evolution of Nitrogen Assimilatory Enzymes in Marine Prasinophytes. Ghoshroy, Sohini; Robertson, Deborah // Journal of Molecular Evolution;Jan2015, Vol. 80 Issue 1, p65 

    Nitrogen assimilation is a highly regulated process requiring metabolic coordination of enzymes and pathways in the cytosol, chloroplast, and mitochondria. Previous studies of prasinophyte genomes revealed that genes encoding nitrate and ammonium transporters have a complex evolutionary history...

  • Chlapsin, a chloroplastidial aspartic proteinase from the green algae Chlamydomonas reinhardtii. Almeida, Carla; Pereira, Cláudia; Costa, Diana; Pereira, Susana; Pissarra, José; Simões, Isaura; Faro, Carlos // Planta;Jul2012, Vol. 236 Issue 1, p283 

    Aspartic proteinases have been extensively characterized in land plants but up to now no evidences for their presence in green algae group have yet been reported in literature. Here we report on the identification of the first (and only) typical aspartic proteinase from Chlamydomonas...

  • New Algal Enzyme Bioassay for the Rapid Assessment of Aquatic Toxicity. Peterson, S. M.; Stauber, J. L. // Bulletin of Environmental Contamination & Toxicology;May96, Vol. 56 Issue 5, p750 

    The article presents a study which describes the development of a rapid toxicity test based on galactosidase enzymatic activity in the green alga. Galactosidase is also known as glucosidase. A three-hour bioassay is compared to a standard 72-hour algal growth inhibition tests. Results of the...

  • An Fe Deficiency Responsive Element with a Core Sequence of TGGCA Regulates the Expression of FEA1 in Chlamydomonas reinharditii. Xiaowen Fei; Eriksson, Mats; Jinghao Yang; Xiaodong Deng // Journal of Biochemistry;Aug2009, Vol. 146 Issue 2, p157 

    Iron is essential to the unicellular green alga Chlamydomonas, but the molecular mechanism for response to iron deficiency remains largely unknown. In previous studies, we have identified FOX1 and ATX1 FEREs (Fe deficiency-responsive elements) as important regulation components of iron response...

  • Identification ofChlamydomonas reinhardtiiRad51C: Recombinational characteristics. Shalguev, V.; Kaboev, O.; Sizova, I.; Hagemann, P.; Lanzov, V. // Molecular Biology;Jan2005, Vol. 39 Issue 1, p98 

    Unicellular green algaChlamydomonas reinhardtiiis a promising model for fundamental and biotechnological research. However, little is known about its system of homologous recombination underlying recombination repair of double-strand breaks. Sequencing of theC. reinhardtiinuclear genome has...

  • Harmful and parasitic unicellular eukaryotes persist in a shallow lake under reconstruction (L. Karla, Greece). Nikouli, Eleni; Kormas, Konstantinos; Berillis, Panagiotis; Karayanni, Hera; Moustaka-Gouni, Maria // Hydrobiologia;Nov2013, Vol. 718 Issue 1, p73 

    The reconstructed Lake Karla, Greece, has been undergoing its water-filling period since November 2009. In this paper, we aimed at investigating whether the unicellular eukaryotes, including the toxic/parasitic ones, that have been found during mass fish kills in the lake (March-April 2010),...

  • Fermentation metabolism and its evolution in algae. Catalanotti, Claudia; Wenqiang Yang; Posewitz, Matthew C.; Grossman, Arthur R. // Frontiers in Plant Science;May2013, Vol. 4, p1 

    Fermentation or anoxic metabolism allows unicellular organisms to colonize environments that become anoxic. Free-living unicellular algae capable of a photoautotrophic lifestyle can also use a range of metabolic circuitry associated with different branches of fermentation metabolism. While algae...

  • Genetic Engineering of the Processing Site of D1 Precursor Protein of Photosystem II Reaction Center in Chlamydomonas reinhardtii. Takahashi, Yuichiro; Utsumi, Keiicbir; Yamamoto, Yumiko; Hatano, Aya; Satoh, Kimiyuki // Plant & Cell Physiology;Mar1996, Vol. 37 Issue 2, p161 

    The D1 protein (D1) of photosystem II (PSII) reaction center is synthesized as a precursor (pD1) and then processed at its carboxyl terminus to establish the function of water cleavage. The amino acid sequence of the carboxyl terminal extension excised by this process is poorly conserved except...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics