Analysis of type I and IV collagens by FT-IR spectroscopy and imaging for a molecular investigation of skeletal muscle connective tissue

Petibois, Cyril; Gouspillou, Gilles; Wehbe, Katia; Delage, Jean-Paul; Déléris, Gérard
December 2006
Analytical & Bioanalytical Chemistry;Dec2006, Vol. 386 Issue 7/8, p1961
Academic Journal
Many muscular diseases result from abnormal organization of connective tissue and/or collagen network formation. Only a few molecular imaging techniques are able to analyze this collagen network by differentiating collagen types. In this study, FT-IR spectroscopy was used to analyze type I and IV collagens, the most important compounds of which are perimysium and endomysium, respectively. Secondary structure of collagen types was determined by curve-fitting the 1,700–1,480 cm−1 spectral interval. Type I collagen could be differentiated from type IV by its higher amounts of triple helix and α-helix, but lower amounts of β-sheets ( P < 0.01). FT-IR imaging was then used to determine structural features of perimysium and endomysium collagen network in bovine Flexor carpi radialis muscle. Secondary structure of proteins contained in perimysium and endomysium was found to be very close to type I and IV collagens, respectively. FT-IR spectroscopy and imaging are thus analytical tools that might be used for investigating biodistribution and assembly of collagen types in connective tissues. [Figure not available: see fulltext.]


Related Articles

  • Ultrasound Stimulation of Type I Collagen and Type III Collagen Expression of Tendon Cells. Huang-Chung Chen; Wen-Chung Tsai; Chu-Wen Tang; Pang, Jong-Hwei S. // AIP Conference Proceedings;2005, Vol. 754 Issue 1, p272 

    This study was designed to determine the effect of ultrasound on tendon cells intrinsic to rat Achilles tendon plated on collagen-coated dishes. Furthermore, the in vitro effect of ultrasound on type I collagen and type III collage expression of tendon cells was investigated. Cultured tendon...

  • A Familial Cutis Laxa Syndrome with Ultrastructural Abnormalities of Collagen and Elastin. Marchase, Patricia; Holbrook, Karen; Pinnell, Sheldon R. // Journal of Investigative Dermatology;Nov80, Vol. 75 Issue 5, p399 

    A familial cutis laxa syndrome is reported in a mother and son. In addition, the son had Klippel-Trenaunay-Weber syndrome, which may be related to his underlying disorder. Ultrastructural examination of skin revealed previously recognized abnormalities of elastic tissue. In addition,...

  • Estudio de fluorescencia de la membrana basal y de estructuras intracelulares en tejidos del conducto coclear del cobayo, teñidas con eosina y observadas con microscopio confocal. Ortega Rangel, Juan; Martín Perea Paz, Juan; Peñaloza López, Yolanda; Espinosa Villanueva, Jesús // Anales de Otorrinolaringología Mexicana;sep-nov2006, Vol. 51 Issue 4, p155 

    BACKGROUND The basal membrane is a structure of the extracellular matrix of the internal environment, and it separates epithelial membranes of the connective tissue. Components of the cochlear duct, such as the spiral limbo, the Corti's Organ and the vascular groove, are integrated by these...

  • Fibre-Matrix Interaction in Soft Tissue. Zaoyang Guo // AIP Conference Proceedings;5/21/2010, Vol. 1233 Issue 1, p1249 

    Although the mechanical behaviour of soft tissue has been extensively studied, the interaction between the collagen fibres and the ground matrix has not been well understood and is therefore ignored by most constitutive models of soft tissue. In this paper, the human annulus fibrosus is used as...

  • Long-term outcome after use of an acellular collagen matrix in a finger degloving injury. Bravo, Elena; Nunez-Villaveiran, Teresa // European Journal of Plastic Surgery;Dec2014, Vol. 37 Issue 12, p687 

    In ring avulsion injuries, replantation may not always be possible because of the extent of the injury or the loss of the severed part. Under these circumstances, other treatment options should be considered. We describe our 5-year follow-up experience after the coverage of a complete digital...

  • TAK1 Is Required for Dermal Wound Healing and Homeostasis. Guo, Fen; Hutchenreuther, James; Carter, David E; Leask, Andrew // Journal of Investigative Dermatology;Jun2013, Vol. 133 Issue 6, p1646 

    Dermal connective tissue is a supportive structure required for skin's barrier function; dysregulated dermal homeostasis results in chronic wounds and fibrotic diseases. The multifunctional cytokine transforming growth factor (TGF) β promotes connective tissue deposition, repair, and...

  • In Vitro and In Vivo Studies with Collagen/Hydroxyapatite Implants. Marouf, Hussein A.; Quayle, Alan A.; Sloan, Philip // International Journal of Oral & Maxillofacial Implants;Summer1990, Vol. 5 Issue 2, p82 

    There is limited literature describing the early events that follow subperiosteal suprabony implantation of particulate hydroxyapatite (HA)/collagen composite grafts. This paper investigates, both in vitro and in vivo, the influence of a water-based environment on compressed collagen fiber...

  • Ex vivo evaluation of antifibrotic compounds in skin scarring: EGCG and silencing of PAI-1 independently inhibit growth and induce keloid shrinkage. Syed, Farhatullah; Bagabir, Rania A; Paus, Ralf; Bayat, Ardeshir // Laboratory Investigation (00236837);Aug2013, Vol. 93 Issue 8, p946 

    Keloid disease (KD) is a common fibroproliferative disorder of unknown etiopathogenesis. Its unique occurrence in human skin and lack of animal models pose challenges for KD research. The lack of a suitable model in KD and over-reliance on cell culture has hampered the progress in developing new...

  • Structure of the human tibialis posterior tendon. Petersen, Wolf; Hohmann, Gerrit; Pufe, Thomas; Tsokos, Michael; Zantop, Thore; Paulsen, Friedrich; Tillmann, Bernhard // Archives of Orthopaedic & Trauma Surgery;May2004, Vol. 124 Issue 4, p237 

    Background. The most common site of rupture of the posterior tibial tendon is the retromalleolar region where the tendon changes its direction of pull. The aim of this study was to characterize the tissue of the gliding zone of the tibialis posterior tendon to gain further knowledge about...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics