TITLE

Determination of homocitrulline in urine of patients with HHH syndrome by liquid chromatography tandem mass spectrometry

AUTHOR(S)
Al-Dirbashi, Osama Y.; Al-Hassnan, Zuhair N.; Rashed, Mohamed S.
PUB. DATE
December 2006
SOURCE
Analytical & Bioanalytical Chemistry;Dec2006, Vol. 386 Issue 7/8, p2013
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A liquid chromatography tandem mass spectrometric method is described for the analysis of homocitrulline in human urine, a key metabolite in the differential diagnosis of hyperammonemia, hyperornithinemia, homocitrullinuria (HHH) syndrome. Urine samples were prepared by mere five-fold dilution with a mixture of internal standards (2H2-citrulline and 2H3-creatinine) used for the simultaneous quantification of creatinine. Analytes were separated on a cyano column and eluted isocratically within seven min. Detection was achieved by monitoring transitions of 190 > 84 and 190 > 127 for homocitrulline, 178 > 115 for 2H2-citrulline, 114 > 44 for creatinine and 117 > 47 for 2H3-creatinine. Calibration curves were linear up to 100 micromol/L. Intraday ( n = 7) and interday ( n = 6) variations were less than 10%. In urine samples from three siblings confirmed to have HHH syndrome, homocitrulline levels were at 13.3 (74), 21.1 (50) and 108.2 (103) mmol/mol creatinine (micromol/L). Control values were 0–9 mmol/mol creatinine ( n = 120). The current method solves specificity issues in homocitrulline determination often encountered with some ninhydrin-based systems (coelution with methionine) and some o-phthalaldehyde-based ones (coelution with taurine), and presents an attractive alternative with a relatively high throughput.
ACCESSION #
23150800

 

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