TITLE

Purification and characterization of a biosurfactant produced by Pseudomonas sp. G11 by asymmetrical flow field-flow fractionation (AsFlFFF)

AUTHOR(S)
Sung Kwang Cho; So Hee Shim; Kyeong Ryang Park; Seong-Ho Choi; Seungho Lee
PUB. DATE
December 2006
SOURCE
Analytical & Bioanalytical Chemistry;Dec2006, Vol. 386 Issue 7/8, p2027
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Three hundred and thirty two bacterial colonies were isolated from soil contaminated by an oil spill. All the bacteria were cultured in a liquid medium individually, and the surface tensions of the media were compared. The bacterium whose culture medium had the lowest surface tension was identified as Pseudomonas sp. G11. A biosurfactant was produced by cultivation of the Pseudomonas sp. G11 in the LB media. For extraction of the biosurfactant, two solvent systems were used ( n-hexane and a 2:1 (v/v) mixture of chloroform/MeOH), and the results were compared. Various experimental conditions (solvent composition, flow rate, etc.) were tested to optimize the analysis of the biosurfactant by asymmetrical flow field-flow fractionation (AsFlFFF). The biosurfactant was successfully separated from the culture medium by AsFlFFF when pure water was used as the carrier. From the retention data, the hydrodynamic diameter ( d H) and molecular weight ( M) of the biosurfactant were determined by AsFlFFF. The molecular weight was determined by using pullulans as the calibration standards. The d H and M were 49 nm and 2.3 × 105 Da when extracted with n-hexane, and 39 nm and 1.13 × 105 Da when extracted with the 2:1 mixture of chloroform/MeOH, respectively. [Figure not available: see fulltext.]
ACCESSION #
23150789

 

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