Determination of ruscogenin in crude Chinese medicines and biological samples by immunoassay

Nan Liu; Xingbing Wen; Jihua Liu; Ming Liang; Huajin Zeng; Yining Lin; Boyang Yu
December 2006
Analytical & Bioanalytical Chemistry;Dec2006, Vol. 386 Issue 6, p1727
Academic Journal
Ruscogenin is a major bioactive steroidal aglycone found in the Chinese medicine, Ophiopogon japonicus. We have developed a quantitative determination of ruscogenin with an indirect enzyme-linked immunosorbent (ELISA) assay using polyclonal antibodies against ruscogenin conjugated with bovine serum albumin. This assay was highly sensitive, and it had considerably less cross-reactivity to diosgenin and sarsasapogenin, but high cross-reactivity to ruscogenin glycosides. The assay was successfully used for the measurement of ruscogenin concentrations in crude Chinese medicines and in biological samples from a pharmacokinetics study of ruscogenin.


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