Observation of salt-induced β-lactoglobulin aggregation using sedimentation field-flow fractionation

Saeseaw, Sudarat; Shiowatana, Juwadee; Siripinyanond, Atitaya
December 2006
Analytical & Bioanalytical Chemistry;Dec2006, Vol. 386 Issue 6, p1681
Academic Journal
Sedimentation field-flow fractionation (SdFFF) was applied in order to characterize particle sizes of β-lactoglobulin aggregates induced by Ca2+ or Zn2+. Aggregation induced by Zn2+ was faster than that induced by Ca2+. Effects of Zn2+ and β-lactoglobulin concentrations, as well as contact time, on the aggregation of β-lactoglobulin were examined. All factors exhibited a combined effect on the size of aggregates, whereby larger aggregates were obtained at increased concentrations of Zn2+ and β-lactoglobulin. At fixed concentrations of 2% (w/v) β-lactoglobulin and 10 mM Zn2+, the particle size of the aggregates increased from 0.19 μm (at 15 min) to 0.38 μm (at 2880 min). Further, a hyphenated technique of SdFFF and inductively coupled plasma–optical emission spectrometry (ICP–OES) was used to examine whether intermolecular ionic bridges take part in salt-induced β-lactoglobulin aggregation. With SdFFF–ICP–OES, protein–cation–protein cross-linkages were observed for β-lactoglobulin aggregation induced by Zn2+, but not for that induced by Ca2+. [Figure not available: see fulltext.]


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