TITLE

Characterization of the coupling of quantum dots and immunoglobulin antibodies

AUTHOR(S)
Xiao-Feng Hua; Tian-Cai Liu; Yuan-Cheng Cao; Bo Liu; Hai-Qiao Wang; Jian-Hao Wang; Zhen-Li Huang; Yuan-Di Zhao
PUB. DATE
December 2006
SOURCE
Analytical & Bioanalytical Chemistry;Dec2006, Vol. 386 Issue 6, p1665
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Water-soluble quantum dots (QDs) were used to label goat anti-human immunoglobulin antibodies (Abs), and the labeling process was characterized by column purification. The QDs obtained in organic solvent were modified with mercaptoacetic acid (MAA) and became water-soluble. These water-soluble QDs were linked to the antibodies using the coupling reagents ethyl-3-(dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS). The linking process was shown to be effective by ultra-filter centrifugation and column purification. After comparing the quantities of Abs and water-soluble QDs involved in the linking reaction via column purification, it was found that a molar Abs:QD ratio of >1.2 resulted in most of the water-soluble QDs becoming covalently linked to the Abs. The circular dichroism (CD) spectra of Abs and QD–Ab conjugates were very similar to each other, indicating that the secondary structure of Abs remained largely intact after the conjugation. Finally, antigen (Ag)–antibody (Ab) recognition reactions perfomed on the surface of a glass slide showed that the conjugate retained the activity of Abs. This work lends support to the idea of linking biomolecules to QDs, and thus should aid the application of QDs to the life sciences. [Figure not available: see fulltext.]
ACCESSION #
23150726

 

Related Articles

  • Magnetic circular dichroism study of CdSe quantum dots. Kuno, M.; Nirmal, M. // Journal of Chemical Physics;3/8/1998, Vol. 108 Issue 10, p4242 

    Examines magnetic circular dichroism study of CdSe quantum dots. Differences between left and right circularly polarized transitions in magnetic field; Determination of the sign and magnitude in exciton g-factor; Sensitivity of exciton g-factor to energy band parameters.

  • Giant Zeeman effects in colloidal diluted magnetic semiconductor quantum dots with homogeneous dopant speciation. Norberg, Nick S.; Gamelin, Daniel R. // Journal of Applied Physics;4/15/2006, Vol. 99 Issue 8, p08M104 

    Ligand field electronic absorption and magnetic circular dichroism (MCD) spectroscopies are used to probe Co2+ dopant speciation in ZnO, ZnSe, and CdS nanocrystals and confirm the high quality of these colloidal diluted magnetic semiconductor quantum dots (DMS-QDs). Measurements of the QD giant...

  • Probing the interactions of CdTe quantum dots with pseudorabies virus. Du, Ting; Cai, Kaimei; Han, Heyou; Fang, Liurong; Liang, Jiangong; Xiao, Shaobo // Scientific Reports;11/13/2015, p16403 

    Quantum dots (QDs) have become one of the most promising luminescent materials for tracking viral infection in living cells. However, several issues regarding how QDs interact with the virus remain unresolved. Herein, the effects of Glutathione (GSH) capped CdTe QDs on virus were investigated by...

  • Crystallographic and magneto-optical studies of nanoscaled MnSb dots grown on GaAs. Mizuguchi, M.; Akinaga, H.; Ono, K.; Oshima, M. // Applied Physics Letters;3/27/2000, Vol. 76 Issue 13 

    MnSb ultrathin films with the nominal thickness of 0-1.40 nm were grown on sulfur passivated GaAs substrates by molecular beam epitaxy. Atomic force microscopy analysis showed that MnSb formed nanosize clusters on the substrate, and the coalescence of the clusters occurred at the nominal...

  • Direct measurement of single-dot coercivity and statistical analysis of switching field distribution in bit-patterned media using scanning hard-X-ray nanoprobe. Motohiro Suzuki; Yuji Kondo; Jun Ariake // Journal of Applied Physics;2016, Vol. 120 Issue 14, p1 

    To investigate the possible sources of the switching field distribution in bit-patterned media, we applied a scanning hard-X-ray nanoprobe technique based on X-ray magnetic circular dichroism spectroscopy to an array of Co-Pt dots with the typical diameters of 200 nm. Element-specific...

  • Biophysical studies of an NAD(P)-dependent aldehyde dehydrogenase from Bacillus licheniformis. Lo, Huei-Fen; Su, Jian-Yu; Chen, Hsiang-Ling; Chen, Jui-Chang; Lin, Long-Liu // European Biophysics Journal;Oct2011, Vol. 40 Issue 10, p1131 

    Aldehyde dehydrogenase (ALDH) catalyzes the conversion of aldehydes to the corresponding acids by means of an NAD(P)-dependent virtually irreversible reaction. In this investigation, the biophysical properties of a recombinant Bacillus licheniformis ALDH ( BlALDH) were characterized in detail by...

  • Development of a caspase-3 antibody as a tool for detecting apoptosis in cells from rainbow trout (Oncorhynchus mykiss). Rojas, Verónica; Guzmán, Fanny; Valenzuela, Cristián; Marshall, Sergio H.; Mercado, Luis // Electronic Journal of Biotechnology;9/15/2012, Vol. 15 Issue 5, p1 

    Background: Apoptosis is an active cell death process mediated by caspases activation, in which different extrinsic or intrinsic signalling pathways result in direct activation of effector caspases. Caspase-3 is considered to be the most important of the executioner caspases, which cause the...

  • Plasmodium vivax Antigen Discovery Based on Alpha-Helical Coiled Coil Protein Motif. Céspedes, Nora; Habel, Catherine; Lopez-Perez, Mary; Castellanos, Angélica; Kajava, Andrey V.; Servis, Catherine; Felger, Ingrid; Moret, Remy; Arévalo-Herrera, Myriam; Corradin, Giampietro; Herrera, Sócrates // PLoS ONE;Jun2014, Vol. 9 Issue 6, p1 

    Protein α-helical coiled coil structures that elicit antibody responses, which block critical functions of medically important microorganisms, represent a means for vaccine development. By using bioinformatics algorithms, a total of 50 antigens with α-helical coiled coil motifs orthologous...

  • ON THE MARKET.  // Nature Medicine;Dec2000, Vol. 6 Issue 12, p1410 

    Introduces various biomedical products recent as of December 2000. Nuclei isolation kits; Antibodies; Reagents.

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics