TITLE

Quantitative analysis of enzymatic assays using indoxyl-based substrates

AUTHOR(S)
Fanjul-Bolado, Pablo; González-García, María Begoña; Costa-García, Agustín
PUB. DATE
December 2006
SOURCE
Analytical & Bioanalytical Chemistry;Dec2006, Vol. 386 Issue 6, p1849
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Hydrolysis of indoxyl-based substrates by hydrolytic enzymes is a commonly used semiquantitative detection system that generates a water-insoluble indigo dye which is difficult to quantify. This work describes the quantitative analysis and enzyme kinetics for alkaline phosphatase (AP) and 5-bromo-4-chloro-3-indoxyl phosphate (BCIP) in solution obtained by applying known solubilization methodology from the textiles industry to the enzymatic product. This proposal is based on the reduction of the tetrahalo-indigo blue dye in a basic medium with the aim of generating its aqueous-soluble parent compound termed indigo white, which gives a rich yellow color in solution and is fluorescent. A quantitative ELISA (where a soluble end product is required) is accomplished for first time using BCIP as substrate.
ACCESSION #
23150720

 

Related Articles

  • Purification and Characterization of Alkaline Phosphatase from Pseudomonas sp. Ali, Asmaa; Adel, Noora // Journal of Plant Molecular Biology & Biotechnology;Jun2012, Vol. 3 Issue 2, p11 

    Alakline phospahatase belongs to the class of hydrolase enzymes that catalyzes the hydrolysis of a number of phosphoric acid esters, nucleotides etc. Extracellular Alkaline phosphatase (AlPase) was isolated from Pseudomonas sp., purified to near homogeneity with ion exchange chromatography on...

  • Acid phosphatase and alkaline phosphatase activities in ripening fruit of Musa Paradisiaca L. Agoreyo, B.O. // Plant OMICS: Journal of Plant Molecular Biology & Omics;2010, Vol. 3 Issue 3, p66 

    The activities of non - specific acid phosphatase and alkaline phosphatase were examined during ripening in the fruit of plantain (Musa paradisiaca L. cv. False horn). This study was carried out in order to determine the activities of these phosphohydrolytic enzymes in supplying inorganic...

  • Ultrasound Dyeing Of Polyester Using Natural Colorant From Melastoma Malabathricum l. TENGKU MUNA SHAHEERA, Tuan Zainal Abidin; MOHD ROZI, Ahmad; MUHAMMAD ISMAIL, Ab Kadir; NOR JULIANA, Mohd Yusof; WAN YUNUS, Wan Ahmad // Advanced Materials Research;2016, Vol. 1134, p66 

    This paper reports on the application of natural dyes on polyester fabric. Melastoma malabathricum l. was extracted using conventional method of boiling for an hour. Plain lightweight polyester fabric was treated with sodium hydroxide as an alkaline treatment and Chitosan prior to dyeing in an...

  • IT'S IN YOUR JEANS. Wells, Rhona // Middle East;Feb2003, Issue 331, p56 

    Discusses the art of indigo dyeing in the Middle East. History of indigo dyeing in the Middle East; Uses of Indigo; Effort made to revive the cultivation of indigo in Oman; Process of making dye from indigo used in Oman.

  • A Case of the Omani Blues. Cortright, Linda N. // Wild Fibers;Spring2012, Vol. 9 Issue 1, p46 

    A personal narrative is presented which explores the author's experiences on her quest to find the only Omani who continues to dye with natural indigo.

  • indigo.  // Taber's Cyclopedic Medical Dictionary (2009);2009, Issue 21, p1180 

    A reference entry for "indigo" which refers to a blue dye from plants is presented.

  • 100 years of synthetic indigo.  // Colourage;Oct98, Vol. 45 Issue 10, p41 

    Discusses the abstract of the article titled "100 years of synthetic indigo," published in the periodical "Textiles Magazine".

  • A Novel Method for Recovery of Indigo from Wastewater. Perkins, Warren S.; Baughman, George L.; Etters, J. Nolan // Proceedings of the Annual International Conference & Exhibition ;2003, p297 

    The article discusses a method for wasted indigo recovery from wastewater.

  • A Cargo More Valuable Than Gold. Balfour-Paul, Jenny // dig;Sep2010, Vol. 12 Issue 7, p28 

    This article explores the history of the use of indigo in dyeing clothes and other materials.

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics