TITLE

Expression of the Canavalia brasiliensis Lectin (ConBr) in Tobacco Plants

AUTHOR(S)
Paiva da Silveira Carvalho, Cristina; Oliveira Correia, Tuana; Mara Serafim Ribeiro, Sandra; Cavalcante Silva, Ingrid; Tadeu Abreu de Oliveira, Jose; Barbosa Grangeiro, Thalles
PUB. DATE
October 2006
SOURCE
Protein & Peptide Letters;Oct2006, Vol. 13 Issue 10, p1045
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Tobacco plants were transformed with gene constructs encoding prepro-ConBr (Canavalia brasiliensis lectin). Transgenic plants confirmed by PCR expressed the recombinant protein as revealed by Western blot. However, the apparent molecular mass of the recombinant polypeptide (ca. 34 kDa) was higher than the native lectin (about 30 kDa), showing that further proteolytic processing of pro-ConBr was not detected.
ACCESSION #
22951001

 

Related Articles

  • Increased yield of heterologous viral glycoprotein in the seeds of homozygous transgenic tobacco plants cultivated underground. Tackaberry, Eilleen S.; Prior, Fiona; Bell, Margaret; Tocchi, Monika; Porter, Suzanne; Mehnic, Jelica; Ganz, Peter R.; Sardana, Ravinder; Altosaar, Illimar; Dudani, Anil // Genome;Jun2003, Vol. 46 Issue 3, p521 

    The use of transgenic plants in the production of recombinant proteins for human therapy, including subunit vaccines, is being investigated to evaluate the efficacy and safety of these emerging biopharmaceutical products. We have previously shown that synthesis of recombinant glycoprotein B (gB)...

  • Use of Transgenic Oryzacystatin-I-Expressing Plants Enhances Recombinant Protein Production. Pillay, P.; Kibido, T.; Plessis, M.; Vyver, C.; Beyene, G.; Vorster, B.; Kunert, K.; Schlüter, U. // Applied Biochemistry & Biotechnology;Nov2012, Vol. 168 Issue 6, p1608 

    Plants are an effective and inexpensive host for the production of commercially interesting heterologous recombinant proteins. The Escherichia coli-derived glutathione reductase was transiently expressed as a recombinant model protein in the cytosol of tobacco plants using the technique of leaf...

  • Cytoprotective Effect of Recombinant Human Erythropoietin Produced in Transgenic Tobacco Plants. Kittur, Farooqahmed S.; Bah, Mamudou; Archer-Hartmann, Stephanie; Hung, Chiu-Yueh; Azadi, Parastoo; Ishihara, Mayumi; Sane, David C.; Xie, Jiahua // PLoS ONE;Oct2013, Vol. 8 Issue 10, p1 

    Asialo-erythropoietin, a desialylated form of human erythropoietin (EPO) lacking hematopoietic activity, is receiving increased attention because of its broader protective effects in preclinical models of tissue injury. However, attempts to translate its protective effects into clinical practice...

  • Correla��es entre atividade inseticida e resist�ncia a prote�lise de duas lectinas vegetais glicose/manose. de Freitas, Cleverson Diniz Teixeira; Ramos, Marcio Viana; Souza, Diego Pereira; Marinho-Filho, Jose Delano Barreto; Teixeira, Fabiano Moura; de Oliveira, Jefferson Soares // Comunicata Scientiae;2011, Vol. 2 Issue 1, p34 

    Purified seed lectins from the legumes Canavalia brasiliensis (ConBr) and Cratylia floribunda (CFL) were tested for insecticidal activity against Callosobruchus maculatus (Coleoptera: Bruchidae) and Dysdercus peruvianus (Hemiptera: Pyrrhocoridae). ConBr and CFL caused negative effects on larvae...

  • Malignancy Risk Analysis in Patients with Inadequate Fine Needle Aspiration Cytology (FNAC) of the Thyroid. Al Maqbali, Talib; Tedla, Miroslav; Weickert, Martin O.; Mehanna, Hisham // PLoS ONE;Nov2012, Vol. 7 Issue 11, Special section p1 

    Background: Thyroid fine needle aspiration cytology (FNAC) is the standard diagnostic modality for thyroid nodules. However, it has limitations among which is the incidence of non-diagnostic results (Thy1). Management of cases with repeatedly non-diagnostic FNAC ranges from simple observation to...

  • High-resolution structural insights on the sugar-recognition and fusion tag properties of a versatile β-trefoil lectin domain from the mushroom Laetiporus sulphureus. Angulo, Iván; Acebrón, Iván; de las Rivas, Blanca; Muñoz, Rosario; Rodríguez-Crespo, I; Menéndez, Margarita; García, Pedro; Tateno, Hiroaki; Goldstein, Irwin J; Pérez-Agote, Begoña; Mancheño, José M // Glycobiology;Oct2011, Vol. 21 Issue 10, p1349 

    In this work, we analyzed at high resolution the sugar-binding mode of the recombinant N-terminal ricin-B domain of the hemolytic protein LSLa (LSL150) from the mushroom Laetiporus sulphureus and also provide functional in vitro evidence suggesting that, together with its putative...

  • Expression of human apolipoprotein A-I in Nicotiana tabacum. Chiaiese, Pasquale; Minutolo, Maria; Arciello, Angela; Guglielmini, Fulvio; Piccoli, Renata; Filippone, Edgardo // Biotechnology Letters;Jan2011, Vol. 33 Issue 1, p159 

    Several transgenic tobacco lines expressing human apolipoprotein A-I (ApoA-I) were obtained. Western blot analyses indicated the expression of the recombinant protein in plant organs at various stages of development, including senescent leaves. A cell line expressing human ApoA-I was established...

  • Molecular Cloning and Transgenic Expression of a Synthetic Human Erythropoietin Gene in Tobacco. Sperb, Fernanda; Werlang, Isabel; Margis-Pinheiro, Marcia; Basso, Luiz; Santos, Diógenes; Pasquali, Giancarlo // Applied Biochemistry & Biotechnology;Oct2011, Vol. 165 Issue 2, p652 

    Erythropoietin (EPO) is a hormone belonging to a group of hematopoietic growth factors that control the proliferation and differentiation of bone marrow cells. It induces the production of erythrocytes, thereby increasing the amount of circulating hemoglobin and oxygen. Previous attempts to...

  • Overexpression of a soybean expansin gene, GmEXP1, improves drought tolerance in transgenic tobacco. Thanh Son LO; Hoang Duc LE; Vu Thanh Thanh NGUYEN; Hoang Ha CHU; Van Son LE; Hoang Mau CHU // Turkish Journal of Botany;2015, Vol. 39 Issue 6, p988 

    The EXP1 gene encodes expansin, which has the ability to loosen the plant cell wall. The soybean expansin gene GmEXP1 is activated specifically during the root elongation process, and thus it plays important roles in root development. During the drought period, changes in pressure within the...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics