Expression of the Canavalia brasiliensis Lectin (ConBr) in Tobacco Plants

Paiva da Silveira Carvalho, Cristina; Oliveira Correia, Tuana; Mara Serafim Ribeiro, Sandra; Cavalcante Silva, Ingrid; Tadeu Abreu de Oliveira, Jose; Barbosa Grangeiro, Thalles
October 2006
Protein & Peptide Letters;Oct2006, Vol. 13 Issue 10, p1045
Academic Journal
Tobacco plants were transformed with gene constructs encoding prepro-ConBr (Canavalia brasiliensis lectin). Transgenic plants confirmed by PCR expressed the recombinant protein as revealed by Western blot. However, the apparent molecular mass of the recombinant polypeptide (ca. 34 kDa) was higher than the native lectin (about 30 kDa), showing that further proteolytic processing of pro-ConBr was not detected.


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