TITLE

Development of a bienzyme system for the electrochemical determination of nitrate in ambient air

AUTHOR(S)
Cui, Yue; Barford, John P.; Renneberg, Reinhard
PUB. DATE
December 2006
SOURCE
Analytical & Bioanalytical Chemistry;Dec2006, Vol. 386 Issue 5, p1567
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
This work reports the development of a bienzyme system consisting of salicylate hydroxylase (SHL) and nitrate reductase (NaR) for the electrochemical determination of nitrate. This method measures the concentration of nitrate directly under ambient air without suffering from oxygen interferences. The determination is based on the detection of NADH consumption, and the principle is as follows: NADH initiates the irreversible decarboxylation and hydroxylation of salicylate by SHL in the presence of oxygen to produce catechol, which results in a detectable signal due to its oxidation at the working electrode; the second enzyme, NaR, in the presence of nitrate, reduced the availability of NADH, and consequently, the current difference after the injection of nitrate is proportional to its concentration. This method shows high performance characteristics for nitrate determination with a broad detection range between 10 μM and 1,000 μM, a short measuring time of around 5 min, and a simple operation without sample pretreatment by inert gas purge or oxygen scavenger. [Figure not available: see fulltext.]
ACCESSION #
22897037

 

Related Articles

  • NADH-cytochrome b5 reductase in a Turkish family with recessive congenital methaemoglobinaemia type I. Percy, M. J.; Aslan, D. // Journal of Clinical Pathology;Oct2008, Vol. 61 Issue 10, p1122 

    The development of cyanosis at birth, the so-called blue baby syndrome, alerts paediatricians to the presence of congenital heart disease. In rare cases where the arterial blood gas analysis is normal the cyanosis is a consequence of methaemoglobinaemia. There are three distinct origins of...

  • Troubled waters. Montgomery, Jenny // Indiana Lawyer;12/21/2011, Vol. 22 Issue 21, p8A 

    The article focuses on phosphorus excess which causes algal blooms and water pollution in Indiana. It says that Steuben commissioners held a public hearing with the Office of the Indiana State Chemist restricting the use of phosphorus fertilizers, but still allowed in agricultural application....

  • Neurometabolic Disorder With Microcephaly, Dystonia, and Central Cyanosis Masquerading as Cerebral Palsy. Devadathan, Kalpana; Sreedharan, Mini; Kunju, P. A. Mohammed; Sarasam, Sanuja; Colah, Roshan B. // Journal of Child Neurology;Nov2014, Vol. 29 Issue 11, pNP139 

    Many neurodegenerative diseases can be misdiagnosed as cerebral palsy. The correct diagnosis is reached when the condition recurs in families or when there are specific clinical signs. The clinical and imaging features of 3 children, from 2 unrelated families, presenting with global...

  • Call for action on algae. SYLVESTER, RON // Ohio Farmer;Nov2014, Vol. 310 Issue 11, p12 

    The article reports on the efforts of Ohio Farmers Union (OFU) to reduce the algal blooms present in Lake Erie by gathering data related to the source of algal blooms according to Joe Logan, president of the OFU. Topics discussed include views of Jeff Reutter, director of Ohio Sea Grant, on...

  • Experimental dynamics of nitrate uptake by winter phytoplankton assemblages. LÓPEZ-ÁLVAREZ, MARCOS; ACUÑA, JOSÉ L.; SOSTRES, JORGE A.; ARRONTES, JULIO // Journal of Plankton Research;Jun2010, Vol. 32 Issue 6, p885 

    The scope for the development of winter blooms depends on phytoplankton response time to transient calm periods and on grazing pressure. We examined the dynamics of nitrate uptake by winter phytoplankton in experimental microcosms, as a proxy for growth dynamics. Natural phytoplankton...

  • 11. The Role of Nitrates in the Eutrophication and Acidification of Surface Waters. Hornung, M. // Managing Risks of Nitrates to Humans & the Environment;1999, p155 

    Nitrogen is an essential nutrient in aquatic ecosystems but when nutrient availability increases eutrophication can result. Chemical changes are paralleled by changes in biological productivity, the composition and diversity of biota and consequently in physical conditions of water bodies. The...

  • Regioselective synthesis of 1,3,5-adamantanetriol from 1,3-adamantanediol using Kitasatospora cells. Mitsukura, Koichi; Yamanaka, Naoki; Yoshida, Toyokazu; Nagasawa, Toru // Biotechnology Letters;Sep2012, Vol. 34 Issue 9, p1741 

    Washed cells (62 mg) of Kitasatospora sp. GF12 in 4 ml buffer (pH 7) catalyzed the regioselective hydroxylation of 60 mM 1,3-adamantanediol [1,3-ad(OH)] to 30.9 mM 1,3,5-adamantanetriol [1,3,5-ad(OH)] over 120 h at 24 °C. Glycerol at 400 mM was added to the reaction mixture to recycle the...

  • Lawsuits Target Algae Regs. Hunter, Pam // ENR: Engineering News-Record;3/26/2012, Vol. 268 Issue 9, p6 

    The article looks at lawsuits filed against the U.S. Environmental Protection Agency (EPA) by environmental groups regarding water pollution contributing to algal blooms in the U.S. The U.S. National Association of Clean Water Agencies (NACWA) voiced concerns about the potential financial...

  • A dual conformation of the post-decarboxylation intermediate is associated with distinct enzyme states in mycobacterial KGD (α-ketoglutarate decarboxylase). WAGNER, Tristan; BARILONE, Nathalie; ALZARI, Pedro M.; BELLINZONI, Marco // Biochemical Journal;2/1/2014, Vol. 457 Issue 3, p425 

    α-Ketoacid dehydrogenases are large multi-enzyme machineries that orchestrate the oxidative decarboxylation of α-ketoacids with the concomitant production of acyl-CoA and NADH. The first reaction, catalysed by α-ketoacid decarboxylases (E1 enzymes), needs a thiamine diphosphate cofactor...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics