Optical biochemical sensor for determining hydroperoxides in nonpolar organic liquids as archetype for sensors consisting of amphiphilic conetworks as immobilisation matrices

Hanko, Michael; Bruns, Nico; Tiller, Joerg C.; Heinze, Jürgen
December 2006
Analytical & Bioanalytical Chemistry;Dec2006, Vol. 386 Issue 5, p1273
Academic Journal
This paper reports the successful design of a prototype of an optical biochemical sensor for the determination of hydroperoxides in nonpolar organic liquids. The sensor consists of a matrix of an amphiphilic polymer conetwork (APCN), a novel class of very promising polymeric materials for easy preparation of biochemical sensor matrices. APCNs are characterised by nanoscopic phase separation between the hydrophilic and the hydrophobic phases. For medium ratios of conetwork composition, the domains of both phases are interconnected both on the surface of the conetworks and throughout the bulk. The APCNs have peculiar swelling properties—the hydrophilic phase swells in hydrophilic media and the hydrophobic phase swells in hydrophobic media. In both types of media dissolved reagents can diffuse from the solution into the swollen phase of the polymeric conetwork. This enables loading of the hydrophilic phase of the APCNs with enzymes and indicator reagents by simple impregnation. Hydrophobic analytes can diffuse into the polymeric conetwork via its hydrophobic phase and react with indicator reagents immobilised in the hydrophilic phase at the huge internal interface between the two opposite phases. To prepare the described hydroperoxide-sensitive biosensors, we used APCN films consisting of 58% ( w/ w) poly(2-hydroxyethyl acrylate) (PHEA) as hydrophilic chains and 42% ( w/ w) polydimethylsiloxane (PDMS) as hydrophobic linkers. Horseradish peroxidase (HRP) and diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) as indicator reagent were co-immobilised in this optically clear and transparent matrix. In this feasibility study the conditions investigated were principally those relevant to characterisation of the innovative matrix material and the disposable biosensor produced from it; the biosensor was not optimised. Sensitivity toward tert-butylhydroperoxide ( tBuOOH) dissolved in n-heptane was acceptable, between approximately 1 and at least 50 mmol L−1, even in the dry state. The response time was 1.7 to 5.0 min. No leaching of immobilised reagents was observed during a period of at least one hour. Pre-swelling the sensors with water increased the reaction rate and the total turnover number of the enzyme. In a dry atmosphere at 4 °C the sensors were found to be stable for at least two weeks.


Related Articles

  • Direct optical sensors: principles and selected applications. Gauglitz, Guenter // Analytical & Bioanalytical Chemistry;Jan2005, Vol. 381 Issue 1, p141 

    In the field of bio and chemosensors a large number of detection principles has been published within the last decade. These detection principles are based either on the observation of fluorescence-labelled systems or on direct optical detection in the heterogeneous phase. Direct optical...

  • Low-Coherence Fibre-Optic Interferometric Sensors. Jedrzejewska-Szczerska, M.; Gnyba, M.; Kosmowski, B.B. // Acta Physica Polonica, A.;Nov2011, Vol. 120 Issue 5, p621 

    In this paper low-coherence fibre-optic sensors based on the Fabry-Perot and Michelson interferometer, which were designed and elaborated at our laboratory, are shown. We present results of investigation of the fibre-optic sensors working in both configurations. These results allowed us to...

  • Tapered Optical Fiber Sensor for Label-Free Detection of Biomolecules. Tian, Ye; Wang, Wenhui; Wu, Nan; Zou, Xiaotian; Wang, Xingwei // Sensors (14248220);2011, Vol. 11 Issue 4, p3780 

    This paper presents a fast, highly sensitive and low-cost tapered optical fiber biosensor that enables the label-free detection of biomolecules. The sensor takes advantage of the interference effect between the fiber's first two propagation modes along the taper waist region. The biomolecules...

  • Fiber-optic coupler based refractive index sensor and its application to biosensing. Tazawa, Hidehisa; Kanie, Tomohiko; Katayama, Makoto // Applied Physics Letters;9/10/2007, Vol. 91 Issue 11, p113901 

    A simple and highly sensitive biosensor based on a fiber-optic coupler is developed. The change of refractive index due to biomolecular interaction on the surface of the coupler can be detected as the change of the transmission power. The sensitivity of the sensor is evaluated to be a noise...

  • Fiber-optic flow-through sensor for online monitoring of glucose. Pasic, Alen; Koehler, Hans; Schaupp, Lukas; Pieber, Thomas R.; Klimant, Ingo // Analytical & Bioanalytical Chemistry;Dec2006, Vol. 386 Issue 5, p1293 

    A new microdialysis-based glucose-sensing system with an integrated fiber-optic hybrid sensor is presented. Design and dimensions of the cell are adapted for its coupling with commercially available microdialysis techniques, thereby providing a new system for continuous glucose monitoring. The...

  • Applications of Gradient Index Multimode Interference Structures in the Technology of Optical Sensor. Szewczuk, A.; Błahut, M. // Acta Physica Polonica, A.;Nov2011, Vol. 120 Issue 5, p740 

    The aim of the work is the presentation of operating principle and properties of multimode interference structure devices made in gradient index technology by K+ and Na+ ion exchange method from the point of view of optical sensor design. Numerical analysis was performed using beam propagation...

  • Development of evanescent wave absorbance-based fibre-optic biosensor. Kundu, T.; Sai, V.; Dutta, R.; Titas, S.; Kumar, P.; Mukherjee, S. // Pramana: Journal of Physics;Dec2010, Vol. 75 Issue 6, p1099 

    Development of chemical and biochemical sensors is the current need of the society. In this report, we present our investigation on the development of a label-free fibre-optic biosensor based on evanescent wave absorbance to detect the presence of analytes such as bacteria, virus and some...

  • Fiber-Optic Chemical Nanosensors by Electrostatic Molecular Self-Assembly. Del Villar, Ignacio; Matias, Ignacio R.; Arregui, Francisco J. // Current Analytical Chemistry;Oct2008, Vol. 4 Issue 4, p341 

    A review of optical fiber sensors based on nanostructured coatings fabricated by means of the layer-by-layer electrostatic-self assembly (ESA) method is presented here. The modification of the optical properties of these sensing coatings by the target parameter can induce changes in the...

  • Improving the Sensitivity of Fiber Surface Plasmon Resonance Sensor by Filling Liquid in a Hollow Core Photonic Crystal Fiber. Tan, Zhixin; Li, Xuejin; Chen, Yuzhi; Fan, Ping // Plasmonics;Feb2014, Vol. 9 Issue 1, p167 

    Inspired by the classic theory, we suggest that the performance of a D-shaped fiber optical surface plasmon resonance (SPR) sensor can be improved by manipulating the fiber core mode. To demonstrate this, we propose a novel fiber SPR sensor based on a hollow core photonic crystal fiber with...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics