A microchip sensor for calcium determination

Caglar, P.; Tuncel, S. A.; Malcik, N.; Landers, J. P.; Ferrance, J. P.
December 2006
Analytical & Bioanalytical Chemistry;Dec2006, Vol. 386 Issue 5, p1303
Academic Journal
A newly designed glass-PDMS microchip-based sensor for use in the determination of Ca2+ ions has been developed, utilizing reflectance measurements from arsenazo III (1,8-dihydroxynaphthalene-3,6-disulfonic acid-2,7-bis[(azo-2)-phenyl arsenic acid]) immobilized on the surface of polymer beads. The beads, produced from cross-linked poly( p-chloromethylstyrene) (PCMS), were covalently modified with polyethylenimine (PEI) to which the Arsenazo III could be adsorbed. The maximum amount of Arsenazo III which could be immobilized onto the PEI-attached PCMS beads was found to be 373.71 mg g−1 polymer at pH 1. Once fabricated, the beads were utilized at the detection point of the microfluidic sensor device with a fiber optic assembly for reflectance measurements. Samples were mobilized past the detection point in the sensor where they interact with the immobilized dye. The sensor could be regenerated and re-used by rinsing with HCl solution. The pH, voltage, linear range, and the effect of interfering ions were evaluated for Ca2+ determination using this microchip sensor. At the optimum potential, 0.8 kV, and pH 9.0, the linear range of the microchip sensor was 3.57 × 10−5 – 5.71 × 10−4 M Ca2+, with a limit of detection (LOD) of 2.68 × 10−5 M. The microchip biosensor was then applied for clinical analysis of calcium ions in serum with good results.


Related Articles

  • Electrokinetic control of fluid in plastified laser-printed poly(ethylene terephthalate)-toner microchips. Feng-Yun He; Ai-Lin Liu; Jin-Hua Yuan; Coltro, Wendell Karlos Tomazelli; Carrilho, Emanuel; Xing-Hua Xia // Analytical & Bioanalytical Chemistry;May2005, Vol. 382 Issue 1, p192 

    The application of plastified laser-printed poly(ethylene terephthalate)(PET)-toner microchips to capillary electrophoresis was investigated. Electroosmotic flow was observed in the direction of the cathode for the buffer system studied (phosphate, pH 3-10). Average electroosmotic mobilities of...

  • Spark-plug interferometer senses cylinder pressure. Wallace, John // Laser Focus World;May2003, Vol. 39 Issue 5, p58 

    Reports on the integration of an interferometric fiberoptic sensors into a spark plug to provide pressure sensing within an integrated circuit-engine cylinder by the researchers at Texas A&M University. Fabrication of an intrinsic fiber Fabry-Perot interferometer; Detection of return signal by...

  • Bragg gratings: Optical microchip sensors. Watts, Sam // Nature Photonics;Jul2010, Vol. 4 Issue 7, p433 

    The article presents information on the use of the Bragg gratings technology in optical microchip sensors. The technology is used in planar silica waveguides for providing refractive index for a large number of applications in biochemistry and chemistry as well as for optical sensing of the...

  • Fabry-Pérot Fiber-Optic Sensors for Physical Parameters Measurement in Challenging Conditions. Pinet, Éric // Journal of Sensors;2009, p1 

    Optical fiber sensors have unique advantages and distinctive features that make them very attractive for many applications especially those involving challenging conditions where other traditional electrical sensors usually fail. Among the commercially available optical fiber sensors, the...

  • Microchip-based electrochemical detection for monitoring cellular systems. Johnson, Alicia; Selimovic, Asmira; Martin, R. // Analytical & Bioanalytical Chemistry;Apr2013, Vol. 405 Issue 10, p3013 

    The use of microchip devices to study cellular systems is a rapidly growing research area. There are numerous advantages of using on-chip integrated electrodes to monitor various cellular processes. The purpose of this review is to give examples of advancements in microchip-based cellular...

  • Smart Integrated Sensor for Multiple Detections of Glucose and L-Lactate Using On-Chip Electrochemical System. Yamazaki, Tomoyuki; Ikeda, Takaaki; Byounghyun Lim; Okumura, Koichi; Ishida, Makoto; Sawada, Kazuaki // Journal of Sensors;2011, p1 

    Multiple sensor electrodes, a supplementary electrode, a reference electrode, and signal-processing circuits were integrated on a single chip to develop a chip-shaped electrochemical sensing system. L-lactate and glucose were measured using on-chip working electrodes modified by polyion complex...

  • A comparative study of different microchips for capillary electrophoresis with electrochemical detection. Erro, Eustaquio; Ortiz, Patricia // Journal of Applied Electrochemistry;Dec2014, Vol. 44 Issue 12, p1393 

    This work describes a comparison of the performance of different microchip configuration for microchip capillary electrophoresis with electrochemical detection. Two electrodes gold and multistrand carbon fibers and two microchip construction materials polydimethylsiloxane/polydimethylsiloxane...

  • Poly(dimethylsiloxane) microchip capillary electrophoresis with electrochemical detection for rapid measurement of acetaminophen and its hydrolysate. He, F.V.; Liu, A.L.; Xia, X.H. // Analytical & Bioanalytical Chemistry;Aug2004, Vol. 379 Issue 7/8, p1062 

    Abstract Poly(dimethylsiloxane) microchip capillary electrophoresis with amperometric detection has been used for rapid separation and determination of acetaminophen and its hydrolysate, i.e. p-aminophenol. A Pt ultramicroelectrode with a diameter of 10 µm positioned at the outlet of the...

  • Integrated Microcalorimeters Using Ir TES And Sn Mushroom Absorbers. Chen, C.; Bogorin, D.; Galeazzi, M. // AIP Conference Proceedings;2006, Vol. 850 Issue 1, p1609 

    Cryogenic microcalorimeters have the potential to meet the requirements of future x-ray missions. The University of Miami has recently started a program to fabricate fully integrated microcalorimeter arrays. We deposit high purity iridium thin film as Transition Edge Sensors (TES). We chose...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics