TITLE

Application and validation of a urinary methadone metabolite (EDDP) immunoassay to monitor methadone compliance

AUTHOR(S)
George, S.; Parmar, S.; Meadway, C.; Braithwaite, R. A.
PUB. DATE
May 2000
SOURCE
Annals of Clinical Biochemistry;May2000, Vol. 37 Issue 3, p350
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A total of 1381 urine specimens were screened using a Microgenics CEDIA urinary primary methadone metabolite (2-ethylidene-1,5-dimethyl-3,3- diphenylpyrrolidine; EDDP) immunoassay (cut-off calibrator concentration of 100 µg/L) in combination with a Dade Behring EMIT urinary methadone immunoassay (cut-off calibrator concentration of 300 µg/L). Of these, 642 (46%) were found to be positive using the EDDP assay but only 541 (39%) were found to be positive by the methadone assay. Out of the 108 specimens which were EDDP-positive but negative by the methadone assay, 47 (7%) could not be confirmed as positive using the routine in-house method of gas chromatography incorporating nitrogen-specific detection. Of these 47 results, 38 were re-screened using a more sensitive gas chromatography-mass spectrometry (GC-MS) technique, which demonstrated the presence of EDDP in every case. There was insufficient specimen to analyse the remaining nine samples. There were seven specimens which gave negative results by the EDDP and GC-MS assays but which had methadone concentrations ranging from 4000 to 37 500 µg/L. These were therefore presumed to be 'spiked' with methadone, i.e. to have had methadone added to the specimen to yield positive screening results and simulate compliance. It is concluded that the Microgenics CEDIA EDDP assay is a sensitive and reliable technique to determine the compliance of subjects prescribed methadone for opiate detoxification and maintenance.
ACCESSION #
22630330

 

Related Articles

  • Comparison of Cozart® Microplate ELISA and GC-MS Detection of Methadone and Metabolites in Human Hair. Cooper, Gail; Wilson, Lisa; Reid, Claire; Baldwin, Dene; Hand, Chris; Spiehler, Vina // Journal of Analytical Toxicology;Oct2005, Vol. 29 Issue 7, p678 

    Determines the performance characteristics of the Cozart Methadone Microplate enzyme-linked immunosorbent assay for the detection of methadone and methadone metabolites in hair specimens. Extraction of hair samples by sonication in methanol; Use of gas chromatography-mass spectrometry analysis;...

  • Validation of an Extraction and Gas Chromatography-Mass Spectrometry Quantification Method for Cocaine, Methadone, and Morphine in Postmortem Adipose Tissue. CoIucci, A. P.; Aventaggiato, L.; Centrone, M.; Gagliano-Candela, R. // Journal of Analytical Toxicology;Jul/Aug2010, Vol. 34 Issue 6, p342 

    The article presents a study which aims to confirm and develop an accurate and sensitive extraction method for cocaine and metabolites, morphine, and methadone detection by gas chromatography-mass spectrometry (GC-MS) in postmortem adipose tissues. The tissue samples were from the University of...

  • Development and validation of a solid-phase extraction gas chromatography-mass spectrometry method for the simultaneous quantification of methadone, heroin, cocaine and metabolites in sweat. Brunet, Bertrand; Barnes, Allan; Scheidweiler, Karl; Mura, Patrick; Huestis, Marilyn // Analytical & Bioanalytical Chemistry;Sep2008, Vol. 392 Issue 1/2, p115 

    A sensitive and specific method is presented to simultaneously quantify methadone, heroin, cocaine and metabolites in sweat. Drugs were eluted from sweat patches with sodium acetate buffer, followed by SPE and quantification by GC/MS with electron impact ionization and selected ion monitoring....

  • Quantitation of Methadone and Metabolite in Patients under Maintenance Treatment. Diong, Shiau Hui; Mohd Yusoff, Nor Shuhadah; Sim, Maw Shin; Raja Aziddin, Raja Elina; Chik, Zamri; Rajan, Poppy; Abdul Rashid, Rusdi; Chemi, Norliza; Mohamed, Zahurin // Journal of Analytical Toxicology;Nov2014, Vol. 38 Issue 9, p660 

    Gas chromatography–mass spectrometry quantitative method was developed to monitor concentrations of methadone and its metabolite 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) in plasma and urine of patients. The developed method was simple, accurate and reproducible to quantify...

  • Monitoring to help break addictions. FORD, JASON // Engineer (00137758);Jan2014, Vol. 296 Issue 7847, p7 

    The article discusses a project which aims to develop improved patient management tools to help with the delivery of more cost-effective and convenient opioid substitution treatment (OST) programmes. The OST programme involves the prescription of a regular dose of opioid replacement like...

  • Tandem DARTâ„¢ MS Methods for Methadone Analysis in Unprocessed Urine. Beck, Rachel; Carter, Patrick; Shonsey, Erin; Graves, David // Journal of Analytical Toxicology;Mar2016, Vol. 40 Issue 2, p140 

    Current methods of methadone analysis in untreated urine are traditionally limited to enzyme immunoassays (EIA) while confirmation techniques require specimen processing (i.e., sample clean-up) before analyzing by gas or liquid chromatography coupled with mass spectrometry (GC-MS or LC-MS-MS)....

  • Comparison of an Automated and Point-of-Care Immunoassay to GC—MS for Urine Oxycodone Testing in the Clinical Laboratory. Haller, Christine A.; Stone, Judith; Burke, Vicki; Branch, Jean; Chen, Kathy; Gross, Susan // Journal of Analytical Toxicology;Mar2006, Vol. 30 Issue 2, p106 

    The study compares the analytical performance of two immunoassay methods for the detection of oxycodone and metabolites in urine to that of a previously validated gas chromatography-mass spectrometry method for free oxycodone. It aims to identify, validate, and implement a reliable testing...

  • Autochthonous Human Schistosomiasis, Malaysia. Latif, Baha; Chong Chin Heo; Razuin, Rahimi; Shamalaa, Devi V.; Tappe, Dennis // Emerging Infectious Diseases;Aug2013, Vol. 19 Issue 8, p1340 

    The article reports after 30 years the histologic finding of Schistosomiasis malayensis-like eggs in the liver of a Malay man and discuss public health implications in Malaysia. An autopsy was conducted and gross pathology showed a normal heart, kidneys, and brain. Methadone and a derivative...

  • Evaluation of the Cedia® Heroin Metabolite (6-AM) Immunoassay with Urine Specimens from a Criminal Justice Drug-Testing Program. Jenkins, Amanda J.; Lavins, Eric S.; Snyder, Ann // Journal of Analytical Toxicology;Apr2005, Vol. 29 Issue 3, p201 

    Evaluates the Cedia Heroin metabolite immunoassay with urine specimens from a criminal justice drug-testing program. Confirmation rate for the enzyme immunoassay; Use of gas chromatography-mass spectrometric analysis; Cutoff concentration.

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics